Map4k4 (NM_001106904) Rat Tagged ORF Clone

CAT#: RR201398

Map4k4 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), (10 ug)

Product Images

Circular map for RR201398

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map4k4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201398 representing NM_001106904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTCACCGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTGAAGAAATATTCTCATC
  ATAGAAATATTGCAACATACTATGGTGCTTTCATTAAGAAGAGCCCTCCAGGACACGATGACCAGCTCTG
  GCTCGTTATGGAGTTTTGCGGGGCTGGTTCCATCACAGACCTTGTGAAGAACACCAAAGGGAACACGCTC
  AAAGAAGACTGGATTGCTTACATCTCCCGGGAAATCCTCAGGGGGTTGGCCCATCTCCACATTCACCATG
  TCATTCACCGAGACATCAAGGGGCAGAACGTGCTGCTGACTGAGAACGCGGAGGTGAAACTTGTTGATTT
  TGGTGTAAGCGCTCAGCTGGACAGGACGGTTGGACGGAGAAATACATTCATAGGCACACCATACTGGATG
  GCTCCGGAGGTCATCGCCTGCGACGAGAATCCAGATGCCACTTACGACTACAGAAGTGACCTCTGGTCTT
  GTGGCATCACGGCCATCGAGATGGCAGAAGGAGCCCCCCCTCTCTGTGACATGCATCCAATGAGAGCGCT
  GTTTCTCATCCCCAGAAACCCTCCTCCCAGGCTGAAGTCAAAAAAATGGTCAAAGAAATTTTTCAGCTTC
  ATAGAAGGCTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAGCTTTTAAAACACCCTTTCA
  TAAGGGATCAGCCCAATGAAAGGCAGGTTCGAATCCAGCTTAAGGATCACATAGACCGGACCAGGAAGAA
  GAGAGGCGAGAAAGATGAGACGGAGTACGAGTACAGTGGCAGTGAGGAGGAGGAGGAGGAAGTGCCTGAG
  CAGGAGGGAGAGCCAAGTTCCATCGTCAATGTGCCTGGAGAGTCGACTCTGCGACGTGATTTCCTGAGGC
  TGCAGCAAGAGAACAAGGAACGGTCTGAGGCTCTGCGGAGACAGCAGCTTCTGCAGGAGCAGCAGCTCCG
  GGAGCAGGAGGAGTATAAGAGGCAGCTGCTGGCTGAGAGGCAGAAGCGGATTGAACAGCAGAAAGAACAG
  AGGAGGCGGCTGGAAGAGCAACAAAGGAGAGAGCGTGAAGCCAGGAGGCAGCAGGAACGTGAGCAGCGGC
  GGCGAGAACAGGAGGAGAAGAGGCGTCTGGAGGAACTGGAAAGGCGGCGTAAGGAAGAGGAAGAGAGGAG
  ACGGGCAGAAGACGAGAAGAGGCGAGTGGAGCGGGAGCAGGAGTACATCAGGCGGCAGCTAGAGGAAGAG
  CAGCGGCACCTGGAGATCCTGCAGCAGCAGCTGCTCCAGGAGCAGGCTATGTTACTGCACGACCACAGGA
  GGCCACACGCACAACAGCCGCCACCCCCGCAGCAGCAGGACAGGAGCAAACCCAGCTATCACGCTCCAGA
  GCCCAAGCCTCACTACGACCCTGCTGACAGAGCTCGGGAGGTACAGTGGTCCCACCTGGCATCTCTCAAG
  AACAATGTCTCCCCTGTCTCGAGATCCCATTCCTTCAGTGACCCTTCTCCCAAATTCGCACACCACCATC
  TCCGCTCTCAGGACCCATGTCCACCTTCCCGCAGTGAGGGGCTCAGTCAGAGCTCTGACTCTAAGTCGGA
  GGTACCTGAGCCCACCCAAAAGGCTTGGTCTAGATCAGACAGTGACGAGGTGCCTCCAAGGGTTCCTGTG
  AGAACGACATCTCGTTCCCCTGTCCTGTCCCGTCGAGATTCTCCACTGCAGGGCAGTGGGCAGCAAAATA
  GCCAAGCAGGACAGAGAAATTCCACCAGCAGTATTGAGCCCCGGCTTCTATGGGAGAGAGTGGAAAAGCT
  GGTCCCCAGGCCAGGCAGTGGCAGCTCTTCAGGGTCCAGCAACTCAGGATCCCAGCCTGGCTCCCATCCT
  GGGTCTCAGAGTGGCTCTGGTGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGTTCTCCATCTC
  AGCGCCTTGAAAATGCAGCCAAAAAACCTGAAGATAAGAAAGAAGTATTTCGACCTCTCAAACCTGCTGT
  AAGGATTGACTTGACTGCGTTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTGCGGCCACCTCACAAAGTG
  ACAGACTATTCCTCCTCCAGTGAGGAGTCTGGGACCACGGATGAGGAGGAGGAGGATGTGGAACAGGAGG
  GGGCTGATGATTCCACCTCGGGGCCAGAGGACACCAGAGCAGCGTCGTCTCTGAATCTGAGCAACGGTGA
  GACAGAATCTGTGAAAACAATGATTGTTCATGATGACGTAGAAAGTGAACCAGCCATGACCCCATCCAAG
  GAGGGCACCCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCT
  CCTTTACACCTTTTATAGACCCCAGGTTACTACAGATCTCTCCATCTAGTGGAACAACAGTTACTTCCGT
  TGTGGGATTTTCCTGTGATGGGCTGAGACCAGAAGCCATAAGGCAAGATCCTACTCGGAAAGGCTCAGTG
  GTCAATGTGAACCCCACGAACACTAGGCCACAGAGTGATACCCCCGAGATTCGTAAATACAAGAAGAGAT
  TTAACTCAGAGATCCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTTGGCACAGAGAGTGGCCTGAT
  GCTCTTGGACAGAAGTGGCCAAGGGAAAGTGTACCCTCTGATCAGCCGGAGACGGTTCCAGCAAATGGAT
  GTACTTGAGGGCCTGAATGTCCTGGTGACAATATCTGGCAAAAAGGATAAGTTACGCGTCTACTATTTAT
  CCTGGTTAAGAAATAAAATACTTCATAATGATCCGGAAGTTGAGAAGAAGCAGGGGTGGACCACCGTGGG
  CGACCTGGAAGGGTGCGTGCACTATAAAGTCGTAAAATATGAAAGAATCAAGTTTCTGGTAATCGCTTTG
  AAGAGTTCTGTGGAAGTCTATGCATGGGCACCGAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTG
  GAGAACTGGTACATAAGCCATTACTGGTGGATCTCACTGTCGAGGAAGGCCAGAGGTTGAAAGTGATCTA
  TGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCCGGCTCAGTCTATGACATTTATCTACCAACA
  CACATTCAGTGTACCATCAAACCCCATGCAATCATTATCCTCCCCAACACGGATGGAATGGAGCTTCTGG
  TGTGCTACGAAGATGAAGGGGTTTATGTGAACACGTACGGAAGAATCACCAAGGACGTGGTTCTGCAGTG
  GGGAGAGATGCCAACATCTGTAGCATACATCCGGTCGAATCAGACAATGGGCTGGGGAGAGAAGGCAATA
  GAGATACGCTCTGTGGAAACTGGTCATTTGGACGGTGTGTTTATGCACAAAAGGGCTCAGAGACTAAAGT
  TCCTGTGTGAACGTAATGACAAGGTGTTCTTTGCTTCCGTCCGCTCTGGCGGCAGCAGCCAGGTCTACTT
  CATGACCTTAGGCAGGACTTCCCTTCTGAGCTGGCATGACCTTAGGCAGGACTTCCCTTCTGAGCTGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201398 representing NM_001106904
  Red=Cloning site Green=Tags(s)
  
  MDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTL
  KEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWM
  APEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSF
  IEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPE
  QEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQ
  RRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEDEKRRVEREQEYIRRQLEEE
  QRHLEILQQQLLQEQAMLLHDHRRPHAQQPPPPQQQDRSKPSYHAPEPKPHYDPADRAREVQWSHLASLK
  NNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPV
  RTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHP
  GSQSGSGERFRVRSSSKSEGSPSQRLENAAKKPEDKKEVFRPLKPAVRIDLTALAKELRAVEDVRPPHKV
  TDYSSSSEESGTTDEEEEDVEQEGADDSTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSK
  EGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSV
  VNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMD
  VLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIAL
  KSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPT
  HIQCTIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAI
  EIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSWHDLRQDFPSEL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106904
• ORF Size: 3534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 5254 bp
• RefSeq ORF: 3537 bp
• Locus ID: 301363
• Cytogenetics: 9q22
• MW: 135.1 kDa