Chka (NM_017127) Rat Tagged ORF Clone

CAT#: RR201253

Chka (Myc-DDK-tagged ORF) - Rat choline kinase alpha (Chka), (10 ug)

Product Images

Circular map for RR201253

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chka
• Synonyms: Chk; CK-R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201253 representing NM_017127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACCAAGTTCTGCACCGGGGGCGAGGCCGAGCCGTCCCCGCTTGGGCTGCTGCTGAGCTGCGGTG
  GCAGCGCTGCCCCGACGCCCGGCGTAGGGCAGCAGCGCGATGCCGCAGGCGAGCTGGAGTCCAAGCAGCT
  TGGTGGCCGGTCCCAACCTCTCGCGCTGCCGCCGCCACCACCGCCGCCCCTGCCGCTGCCCCCGCCGCCA
  TCACCGCCGCTAGCGGACGAACAACCCGAGCCCCGGACGCGGCGCAGGGCCTACCTGTGGTGCAAGGAAT
  TCCTGCCCGGAGCCTGGAGGGGCCTTCGCGAGGACCAGTTCCACATCAGTGTCATCAGGGGTGGTCTCAG
  TAACATGCTGTTCCAGTGTTCCTTGCCAGACTCCATAGCCAGTGTTGGTGATGAACCTCGGAAAGTGCTC
  TTGCGACTGTATGGGGCAATCTTAAAGATGGGGGCTGAAGCAATGGTTCTGGAGAGTGTTATGTTTGCCA
  TTCTTGCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTTCCGCAAGGCCGACTGGAGCAGTTTAT
  CCCGAGCCGGCGATTGGACACTGAAGAATTATGTTTACCAGATATTTCTGCAGAAATAGCTGAAAAAATG
  GCCACATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAAAATGGCTTTTTGGAACAATGGAAAAAT
  ACCTGAATCAAGTACTAAGACTTAAATTCAGCAGGGAGGCCAGAGTTCAACAACTGCACAAGTTCCTCTC
  TTACAATCTGCCTCTCGAGCTTGAGAACCTGAGGTCATTGCTGCAGTATACTAGATCCCCAGTTGTGTTT
  TGTCATAATGACTGTCAAGAAGGTAATATCTTATTGTTGGAAGGCCAAGAGAATTCTGAAAAGCAGAAGT
  TGATGCTCATTGACTTTGAATACAGCAGTTACAATTACAGGGGATTTGACATTGGAAATCATTTCTGTGA
  ATGGATGTATGATTATACCTATGAAAAGTATCCTTTCTTCAGAGCAAACATTCAGAAGTATCCTACCCGA
  AAACAACAGCTCCATTTTATTTCAAGTTACTTGACTACATTCCAAAATGATTTTGAAAGCCTCAGCAGTG
  AAGAGCAGTCTGCTACAAAAGAAGACATGTTGCTTGAAGTCAACAGATTTGCCCTTGCCTCTCATTTCCT
  CTGGGGACTTTGGTCCATTGTACAGGCCAAGATCTCATCCATTGAATTTGGGTACATGGAATATGCCCAA
  GCCAGGTTCGATGCCTACTTTGACCAGAAGAGGAAGCTTGGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201253 representing NM_017127
  Red=Cloning site Green=Tags(s)
  
  MKTKFCTGGEAEPSPLGLLLSCGGSAAPTPGVGQQRDAAGELESKQLGGRSQPLALPPPPPPPLPLPPPP
  SPPLADEQPEPRTRRRAYLWCKEFLPGAWRGLREDQFHISVIRGGLSNMLFQCSLPDSIASVGDEPRKVL
  LRLYGAILKMGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELCLPDISAEIAEKM
  ATFHGMKMPFNKEPKWLFGTMEKYLNQVLRLKFSREARVQQLHKFLSYNLPLELENLRSLLQYTRSPVVF
  CHNDCQEGNILLLEGQENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYTYEKYPFFRANIQKYPTR
  KQQLHFISSYLTTFQNDFESLSSEEQSATKEDMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMEYAQ
  ARFDAYFDQKRKLGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017127
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017127.1, NP_058823.1
• RefSeq Size: 2540 bp
• RefSeq ORF: 1308 bp
• Locus ID: 29194
• UniProt ID: Q01134
• Cytogenetics: 1q43
• MW: 49.7 kDa
• Gene Summary: kinase of the CDP-choline pathway [RGD, Feb 2006]