Gnl2 (NM_001025736) Rat Tagged ORF Clone

CAT#: RR201128

Gnl2 (Myc-DDK-tagged ORF) - Rat guanine nucleotide binding protein-like 2 (nucleolar) (Gnl2), (10 ug)

Product Images

Circular map for RR201128

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gnl2
• Synonyms: RGD1305006
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201128 representing NM_001025736
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGCCCAAGTATAAAGGACGGAGCACCATCAATCGCTCGGCGGCCAGCACCAACCCAGATCGAG
  TACAGGGAGCTGGAGGCCAAAACATGAGGGACCGGGGCACAATTCGGCGCCTGAATATGTACAGGCAAAA
  AGAGCGCAGGAACAGTCGTGGTAAAGTGATTAAGCCACTGCAGTATCAGTCAACTGTGGCTCCTGGCACA
  GTGGCACGAGTGGAGCCGAATATTAAATGGTTTGGAAATACTCGTGTGATCAAGCAGGCATCATTACAAA
  AATTTCAAGAGGAAATGGATAAAGTTATGAAGGATCCATACAAAGTTGTGATGAAACAAAGCAAATTACC
  GATGTCTCTTCTGCACGATCGAGTCCAGCCTCATAACGCAAAGGTCCACATTCTTGATACTGAAAGTTTT
  GAAAGTACATTTGGCCCAAAGTCACAGAGAAAGCGGCCAAACTTGTTTGCAAGTGATATGCAGTCCCTTC
  TAGAAAACGCTGAAATGTCTACTGAGAGCTATGACCAGGGAAAGGACCGTGATTTGGTGACAGAGGACAC
  AGGTGTAAGAAATGAAGCTCAAGAAGAGATATATAAAAAGGGGCAGTCAAAAAGAATATGGGGAGAACTC
  TACAAGGTGATAGACTCATCAGATGTTGTAGTCCAAGTTCTTGACGCTAGAGACCCAATGGGCACTCGTT
  CCTCTCACATCGAAGCTTACTTGAAGAAGGAAAAGCCCTGGAAGCACCTCATTTTTGTACTCAATAAGTG
  TGACCTTGTTCCCACTTGGGCAACAAAACGATGGGTTGCTGTGCTCTCCAAGGACTACCCAACACTGGCT
  TTCCATGCGAGTCTCACTAATCCCTTTGGGAAAGGAGCATTCATTCAGCTTCTGCGGCAGTTTGGGAAGT
  TGCACACAGACAAGAAACAAATCAGTGTTGGGTTCATTGGCTATCCAAATGTAGGCAAGAGCTCTGTGAT
  AAATACATTACGATCCAAGAAAGTTTGCAACGTGGCCCCAATTGCAGGAGAAACAAAGGTCTGGCAATAT
  ATTACCTTGATGCGCCGTATATTCCTGATCGACTGCCCTGGTGTGGTTTACCCATCTGAGGACTCAGAGA
  CCGACATTGTTCTCAAAGGAGTGGTTCAAGTTGAAAAAATTAAAGCTCCTCAAGACCACATTGGTGCTGT
  CCTTGAACGAGCAAAGCCAGAATATATCAGCAAGACATACAAGATTGAGTCCTGGGAGAATGCTGAAGAC
  TTTCTCGAGAAACTAGCTCTCCGCACCGGGAAGTTACTGAAGGGTGGAGAGCCTGACTTGCTGACCGTGA
  GCAAGATGGTGCTCAATGACTGGCAGAGAGGCCGGATCCCTTTCTTTGTCAAGCCTCCCAACGCAGAGAT
  GCCGACCACTCCCCAGCTCCCACCCTCCTCACCATTGGAAGTTACCACAGAAACAACGCAGAACAACCCA
  GAAGAGGAGACCATAGAAACAGAAGTCGAAAGGTCAGACTCTGTCATTGAAAAGGAGCCAGAAGCAGGCT
  GTTCCCAGGATGAAAACTCAGAGATGCAACAGATCCTCGCACGGGTTCGCCAGAACTTTGGTAAAATCAA
  TGTAGGGCCTCAGTTCTCTGCGGACGACCTGGTGCCCGTGGACGACCTGGTGCCCGTGGACTTGTCAGAC
  CTGGAAGATCTGGAAAGCTCTGAGGAGGAGGAGGAGCAGGAGCAGGAGCAGCCAGGAGAGGATGCTGAGG
  AAGAGTGTTCCCGAGACACGCGGGGAGGGCCCGTGGAAGATGACACCAAAGCTGTGATCAGAGCCCTAGA
  TGAGAAGATTGCCAAGTACCAGAGGTTTCTAAATAAAGCCAAAGCTAAAAAGTTCTCTGCCATCAGAATA
  TCCAAAGACTTAAGTGAAAAGGTTTTTGCTAAGTATGAAGAAGAGAAGAAAGCGTCTGCAGAAGTCAGTG
  ATGCAGCACCCACCAAAAGGGCAAGGAAGTGGAATGCACAGGTGGAAGAAGAGCCTTCAGATAAGACTCA
  GAGGATGCTGACGTGTAAGGAACGGAGGAGAGCAGCTCGGCAGCAACAATCCAAAAAAGTCGGTGTGCGC
  TACTATGAAACACACAACGTGAAAAACAGAAACAGGAACAAAAAGAAGACGAATGACTCAGAGGGACAGA
  AACACAGACATAAGAAGTTCAGACAGAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201128 representing NM_001025736
  Red=Cloning site Green=Tags(s)
  
  MVKPKYKGRSTINRSAASTNPDRVQGAGGQNMRDRGTIRRLNMYRQKERRNSRGKVIKPLQYQSTVAPGT
  VARVEPNIKWFGNTRVIKQASLQKFQEEMDKVMKDPYKVVMKQSKLPMSLLHDRVQPHNAKVHILDTESF
  ESTFGPKSQRKRPNLFASDMQSLLENAEMSTESYDQGKDRDLVTEDTGVRNEAQEEIYKKGQSKRIWGEL
  YKVIDSSDVVVQVLDARDPMGTRSSHIEAYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSKDYPTLA
  FHASLTNPFGKGAFIQLLRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQY
  ITLMRRIFLIDCPGVVYPSEDSETDIVLKGVVQVEKIKAPQDHIGAVLERAKPEYISKTYKIESWENAED
  FLEKLALRTGKLLKGGEPDLLTVSKMVLNDWQRGRIPFFVKPPNAEMPTTPQLPPSSPLEVTTETTQNNP
  EEETIETEVERSDSVIEKEPEAGCSQDENSEMQQILARVRQNFGKINVGPQFSADDLVPVDDLVPVDLSD
  LEDLESSEEEEEQEQEQPGEDAEEECSRDTRGGPVEDDTKAVIRALDEKIAKYQRFLNKAKAKKFSAIRI
  SKDLSEKVFAKYEEEKKASAEVSDAAPTKRARKWNAQVEEEPSDKTQRMLTCKERRRAARQQQSKKVGVR
  YYETHNVKNRNRNKKKTNDSEGQKHRHKKFRQKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025736
• ORF Size: 2202 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025736.1, NP_001020907.1
• RefSeq Size: 2328 bp
• RefSeq ORF: 2205 bp
• Locus ID: 362593
• Cytogenetics: 5q36
• MW: 83.9 kDa