Lamb2 (NM_012974) Rat Tagged ORF Clone
CAT#: RR200947
- TrueORF®
Lamb2 (Myc-DDK-tagged ORF) - Rat laminin, beta 2 (Lamb2), (10 ug)
"NM_012974" in other vectors (1)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Lamb2 |
Synonyms | SLAM |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200947 representing NM_012974
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGGGCCTCAGGAAAACCAGGGAGGGGCAGGCAGGGACAGCCTGTGCCATGGGAACTTCGCTTGG GCCTACTTCTAAGTGTGCTGGCTGCCACATTGGCCCAAGTCCCATCCTTGGATGTACCTGGCTGTTCTCG AGGAAGCTGCTATCCAGCCACCGGTGACCTGTTGGTGGGCCGTGCAGACAGACTGACAGCCTCCTCCACG TGTGGCTTGCATAGCCCTCAACCCTACTGTATTGTCAGTCACCTGCAGGATGAAAAGAAGTGCTTCCTGT GTGACTCCCGACGTCCCTTCTCTGCTCGAGACAACCCAAATAGTCACCGGATCCAGAATGTAGTCACTAG CTTTGCGCCACAACGCCGGACGGCCTGGTGGCAGTCGGAGAACGGTGTTCCGATGGTCACCATCCAACTG GACCTGGAAGCTGAGTTTCATTTCACCCACCTCATCATGACCTTCAAGACATTTCGTCCTGCGGCCATGC TGGTGGAGCGCTCTGCAGACTTCGGCCGCACGTGGCGTGTCTACCGATATTTTTCCTACGACTGCGGGGC TGACTTCCCAGGAATCCCGCTGGCCCCGCCGCGTCGCTGGGATGATGTAGTGTGTGAGTCCCGCTACTCA GAAATTGAGCCATCTACTGAAGGCGAGGTCATCTATCGTGTGCTGGATCCTGCTATTCCTATTCCAGACC CCTACAGCTCACGGATTCAGAACCTGTTGAAGATCACCAACCTCCGTGTGAACCTCACCCGGCTTCACAC ACTGGGAGACAACTTGCTTGACCCACGGCGGGAGATCCGGGAAAAATATTATTATGCTCTCTATGAACTT GTCATCCGCGGGAACTGCTTCTGCTATGGACATGCCTCACAGTGTGCGCCTGCACCAGGGGCACCGGCCC ATGCTGAGGGCATGGTACATGGGGCCTGTATTTGCAAGCACAATACTCGTGGGCTCAACTGTGAGCAGTG TCAGGATTTCTATCAGGACCTTCCCTGGCATCCTGCTGAGGATGGCCATACTCACGCCTGTCGGAAGTGT GAGTGCAACGGGCATAGTCATAGCTGCCACTTTGACATGGCTGTGTACCTGGCATCTGGAAACGTAAGTG GAGGTGTCTGCGATGGCTGTCAGCACAACACGGCTGGGCGCCACTGCGAGCTCTGCCGGCCCTTCTTCTA CCGTGACCCAACCAAGGACATGCGGGATCCAGCTGCGTGCCGTCCTTGTGACTGTGACCCAATGGGTTCT CAAGACGGTGGCCGCTGTGATTCCCATGATGACCCTGTGCTAGGTCTGGTCTCAGGCCAGTGTCGCTGCA AAGAACACGTGGTTGGCACTCGCTGCCAGCAATGCCGGGACGGCTTCTTTGGACTTAGTGCCAGCAACCC TCGAGGATGTCAGCGTTGTCAGTGTAACTCAAGGGGCACAGTGCCTGGGGGCACCCCTTGTGACTCCAGT AGTGGAACCTGTTTCTGCAAGCGTCTGGTGACTGGAGACGGCTGTGACCGCTGTCTGCCTGGCCACTGGG GCCTGAGCCATGACCTGCTAGGCTGCCGCCCCTGTGACTGTGATGTGGGCGGTGCCTTGGATCCTCAGTG TGATGAAGCCACGGGTCAGTGCCCGTGCCGCCCACACATGATTGGGCGGCGCTGTGAACAAGTACAGCCT GGCTACTTCCGGCCTTTTCTAGACCATCTAACCTGGGAGGCTGAGGGTGCCCATGGGCAGGTGCTTGAGG TGGTAGAACGTCTGGTGACCAACCGAGAGACTCCGTCCTGGACTGGTGTAGGCTTTGTGCGACTGCGGGA AGGTCAGGAAGTGGAGTTCCTGGTGACCTCTTTGCCTAGGGCCATGGACTATGACCTGCTACTGCGCTGG GAGCCCCAGGTTCCTGAGCAGTGGGCAGAGCTGGAATTGGTGGTGCAGCGCCCAGGGCCTGTGTCTGCAC ACAGTCCGTGTGGGCACGTGCTGCCGAGGGATGACCGCATTCAGGGGATGCTTCACCCAAACACCAGGGT TTTGGTGTTTCCCAGACCTGTCTGCCTTGAGCCTGGTCTCTCCTACAAGCTGAAGTTGAAATTGACTGGA ACAGGGGGACGTGCCCATCCTGAAACCCCATACTCTGGATCTGGAATACTCATTGACTCGCTGGTGCTGC AGCCCCATGTCTTGATGCTAGAGATGTTTAGTGGGGGGGATGCTGCTGCCCTGGAACGCCGTACCACCTT TGAACGCTACCGCTGCCATGAGGAAGGTCTGATGCCCAGCAAGACCCCCCTATCTGAGGCCTGCGTTCCC CTCCTCATCAGTGCGTCCTCCCTGGTCTACAATGGCGCCTTGCCCTGCCAGTGTGACCCTCAAGGCTCAC TGAGTTCCGAATGCAATCCTCATGGTGGCCAGTGCCGGTGCAAGCCTGGAGTGGTTGGACGACGCTGTGA TGCCTGTGCTACTGGCTACTATGGCTTTGGGCCCGCAGGCTGTCAAGCCTGCCAGTGTAGTCCCGACGGA GCACTCAGTGCCCTGTGTGAAGGGACTAGTGGACAGTGTCTCTGCCGAACCGGTGCCTTTGGTCTTCGCT GTGACCACTGTCAACGTGGCCAGTGGGGTTTCCCTAACTGCCGGCCGTGTGTCTGCAACGGGCGTGCAGA TGAGTGTGACGCCCACACAGGCGCTTGCCTGGGCTGCCGTGATTACACAGGGGGCGAGCACTGTGAGAGG TGCATTGCTGGTTTTCATGGGGACCCACGGCTGCCATATGGGGGCCAGTGCCGGCCTTGTCCCTGCCCTG AAGGCCCCGGGAGCCAGCGACACTTTGCTACTTCATGTCACCGGGATGGATATTCCCAGCAAATTGTGTG CCACTGTCGAGCAGGCTACACAGGGCTTCGGTGTGAGGCTTGTGCCCCCGGGCACTTTGGGGACCCATCC AAGCCAGGTGGCAGGTGCCAACTGTGTGAGTGCAGTGGGAACATTGACCCCACGGATCCCGGTGCCTGTG ATCCCCACACGGGGCAATGCTTGCGCTGTTTACACCACACGGAGGGGCCCCACTGTGGCCATTGCAAGCC TGGCTTCCATGGGCAAGCTGCCCGACAGAGCTGTCACCGCTGTACCTGCAACCTTCTGGGCACAGATCCC CAGCGGTGCCCATCTACAGACCTGTGCCATTGTGACCCAAGCACTGGGCAGTGCCCATGCCTCCCCCATG TCCAAGGCCTCAGTTGCGACCGTTGTGCCCCCAACTTTTGGAACTTTACCAGTGGACGTGGCTGCCAGCC TTGTGCTTGTCACCCAAGCCGGGCCAGAGGCCCTACCTGCAATGAGTTCACAGGGCAGTGCCACTGTCAT GCTGGCTTTGGTGGGAGGACCTGTTCTGAGTGCCAAGAGCTCCACTGGGGAGACCCTGGACTGCAGTGCC GCGCCTGTGACTGTGATCCCAGAGGAATAGACAAACCCCAATGTCATCGTTCTACTGGTCACTGTAGCTG CCGCCCAGGCGTGTCTGGCGTGCGCTGCGACCAGTGTGCTCGTGGCTTCTCGGGTGTTTTTCCTGCTTGT CACCCCTGCCATGCATGCTTTGGAGACTGGGATCGTGTGGTACAGGACCTTGCTGCTCGTACGCGGCGCC TGGAGCAGTGGGCTCAGGAATTGCAGCAAACGGGTGTGCTGGGTGCCTTTGAGAGCAGCTTTTTAAACCT GCAGGGGAAGCTGGGAATGGTGCAGGCCATCGTGGCTGCCCGCAACACCTCAGCTGCGTCTACTGCAAAG CTTGTAGAGGCCACAGAGGGACTGCGTCATGAAATTGGGAAGACCACTGAACGCCTGACTCAGTTAGAAG CAGAGCTCACAGATGTACAGGATGAGAACTTCAATGCCAACCATGCACTCAGTGGTCTGGAGAGAGACGG GCTCGCACTTAATCTCACACTGAGGCAGCTTGACCAGCATCTGGATATCCTCAAACACTCAAATTTCTTA GGTGCCTATGACAGCATCCGACATGCCCACAGTCAGTCTACAGAAGCAGAGCGCCGGGCCAATGCCTCCA CCTTTGCAATACCCAGCCCTGTGAGCAACTCAGCAGATACCCGGCGTCGGGCAGAAGTGCTAATGGGTGC CCAAAGAGAGAACTTCAACCGCCAGCACTTGGCCAACCAGCAGGCACTTGGACGGCTGTCCACACACACC CATACCCTGAGCCTGACGGGAGTAAATGAGCTGGTGTGTGGGGCCCCAGGGGACGCACCCTGTGCTACCA GCCCTTGTGGGGGTGCTGGATGTCGGGATGAGGATGGGCAACCCCGCTGTGGTGGCCTCGGTTGCAGTGG AGCAGCAGCCACAGCAGATCTAGCACTGGGCCGGGCTCGGCACACACAGGCAGAGCTGCAGAGAGCGCTG GTAGAAGGTGGTGGCATCCTCAGCCGAGTGTCTGAGACTCGTCGGCAGGCAGAGGAGGCCCAGCAACGAG CACAGGCAGCCCTGGACAAGGCTAATGCTTCTAGGGGCCAAGTGGAGCAGGCCAATCAGGAGCTTCGAGA ACTTATCCAGAATGTGAAGGACTTCCTCAGCCAGGAGGGAGCTGATCCCGACAGTATTGAAATGGTAGCA ACACGGGTGCTAGACATCTCCATCCCAGCCTCGCCTGAGCAGATCCAGCGCCTAGCAAGTGAGATTGCAG AACGTGTCCGAAGCCTGGCGGATGTGGACACAATCCTGGCACATACCATGGGGGATGTGCGTCGGGCTGA ACAGCTACTGCAAGATGCACAGCGGGCACGGAGCCGGGCTGAGGGTGAGAGACAGAAGGCAGAGACAGTC CAAGCGGCACTGGAGGAGGCCCAGAGGGCACAGGGAGCTGCTCAGGGCGCCATCCGGGGAGCAGTGGTTG ACACAAAAAACACAGAGCAGACCCTGCAGCAGGTCCAGGAGAGGATGGCAGGTACAGAGCAGTCTCTGAA CTCTGCTAGTGAGCGGGCTCGGCAGTTACATGCCCTTCTGGAAGCCCTGAAACTGAAAAGGGCAGGAAAC AGCCTGGCAGCATCTACAGCTGAAGAGACAGCAGGCAGTGCCCAGAGCCGTGCCAGGGAGGCTGAGAAAC AGCTACGGGAACAAGTAGGTGACCAGTACCAAACTGTGAGGGCGTTGGCTGAACGGAAGGCTGAAGGTGT TCTGGCCGCACAAGCCAGAGCAGAACAACTGCGGGATGAGGCTCGGGGTCTGTTGCAGGCTGCTCAGGAC AAGTTGCAGCGGCTACAGGAACTGGAGGGCACATATGAAGAGAATGAACGCGAACTGGAGGTCAAAGCGG CTCAGCTGGATGGGCTGGAGGCCAGGATGCGCAGTGTGCTTCAGGCAATCAACCTGCAGGTCCAGATCTA CAACACCTGCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200947 representing NM_012974
Red=Cloning site Green=Tags(s) MEWASGKPGRGRQGQPVPWELRLGLLLSVLAATLAQVPSLDVPGCSRGSCYPATGDLLVGRADRLTASST CGLHSPQPYCIVSHLQDEKKCFLCDSRRPFSARDNPNSHRIQNVVTSFAPQRRTAWWQSENGVPMVTIQL DLEAEFHFTHLIMTFKTFRPAAMLVERSADFGRTWRVYRYFSYDCGADFPGIPLAPPRRWDDVVCESRYS EIEPSTEGEVIYRVLDPAIPIPDPYSSRIQNLLKITNLRVNLTRLHTLGDNLLDPRREIREKYYYALYEL VIRGNCFCYGHASQCAPAPGAPAHAEGMVHGACICKHNTRGLNCEQCQDFYQDLPWHPAEDGHTHACRKC ECNGHSHSCHFDMAVYLASGNVSGGVCDGCQHNTAGRHCELCRPFFYRDPTKDMRDPAACRPCDCDPMGS QDGGRCDSHDDPVLGLVSGQCRCKEHVVGTRCQQCRDGFFGLSASNPRGCQRCQCNSRGTVPGGTPCDSS SGTCFCKRLVTGDGCDRCLPGHWGLSHDLLGCRPCDCDVGGALDPQCDEATGQCPCRPHMIGRRCEQVQP GYFRPFLDHLTWEAEGAHGQVLEVVERLVTNRETPSWTGVGFVRLREGQEVEFLVTSLPRAMDYDLLLRW EPQVPEQWAELELVVQRPGPVSAHSPCGHVLPRDDRIQGMLHPNTRVLVFPRPVCLEPGLSYKLKLKLTG TGGRAHPETPYSGSGILIDSLVLQPHVLMLEMFSGGDAAALERRTTFERYRCHEEGLMPSKTPLSEACVP LLISASSLVYNGALPCQCDPQGSLSSECNPHGGQCRCKPGVVGRRCDACATGYYGFGPAGCQACQCSPDG ALSALCEGTSGQCLCRTGAFGLRCDHCQRGQWGFPNCRPCVCNGRADECDAHTGACLGCRDYTGGEHCER CIAGFHGDPRLPYGGQCRPCPCPEGPGSQRHFATSCHRDGYSQQIVCHCRAGYTGLRCEACAPGHFGDPS KPGGRCQLCECSGNIDPTDPGACDPHTGQCLRCLHHTEGPHCGHCKPGFHGQAARQSCHRCTCNLLGTDP QRCPSTDLCHCDPSTGQCPCLPHVQGLSCDRCAPNFWNFTSGRGCQPCACHPSRARGPTCNEFTGQCHCH AGFGGRTCSECQELHWGDPGLQCRACDCDPRGIDKPQCHRSTGHCSCRPGVSGVRCDQCARGFSGVFPAC HPCHACFGDWDRVVQDLAARTRRLEQWAQELQQTGVLGAFESSFLNLQGKLGMVQAIVAARNTSAASTAK LVEATEGLRHEIGKTTERLTQLEAELTDVQDENFNANHALSGLERDGLALNLTLRQLDQHLDILKHSNFL GAYDSIRHAHSQSTEAERRANASTFAIPSPVSNSADTRRRAEVLMGAQRENFNRQHLANQQALGRLSTHT HTLSLTGVNELVCGAPGDAPCATSPCGGAGCRDEDGQPRCGGLGCSGAAATADLALGRARHTQAELQRAL VEGGGILSRVSETRRQAEEAQQRAQAALDKANASRGQVEQANQELRELIQNVKDFLSQEGADPDSIEMVA TRVLDISIPASPEQIQRLASEIAERVRSLADVDTILAHTMGDVRRAEQLLQDAQRARSRAEGERQKAETV QAALEEAQRAQGAAQGAIRGAVVDTKNTEQTLQQVQERMAGTEQSLNSASERARQLHALLEALKLKRAGN SLAASTAEETAGSAQSRAREAEKQLREQVGDQYQTVRALAERKAEGVLAAQARAEQLRDEARGLLQAAQD KLQRLQELEGTYEENERELEVKAAQLDGLEARMRSVLQAINLQVQIYNTCQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012974 |
ORF Size | 5403 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012974.1, NP_037106.1 |
RefSeq Size | 5581 bp |
RefSeq ORF | 5406 bp |
Locus ID | 25473 |
UniProt ID | P15800 |
Cytogenetics | 8q32 |
MW | 196.5 kDa |
Gene Summary | a component of the basal lamina; may be involved in motor neuron function [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200947 | Lamb2 (untagged ORF) - Rat laminin, beta 2 (Lamb2), (10 ug) |
USD 1,666.00 |
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