Zp1 (NM_053509) Rat Tagged ORF Clone
CAT#: RR200920
- TrueORF®
Zp1 (Myc-DDK-tagged ORF) - Rat zona pellucida glycoprotein 1 (sperm receptor) (Zp1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_053509" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Zp1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200920 representing NM_053509
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGGGGTTGTTTTGTGGTCCTGCTTCTGCTGGTGGCAGCTCCCCTGAGATTGGGTCAGCATCTGC ATCTCAAGCCTGGCTTTCAATACAGCTATGACTGTGGGGTACAGGGCATGCAGCTGCTGGTGTTCCCCAG GCCAAACCAGACTATCCAATTCAAGGTGCTGGATGAATTTGGGAACCGGTTTGAGGTGAATAACTGCTCT ATCTGCTACCACTGGGTGATCAGTGAGGCCCAGAAGCCCGCAGTGTTCTCAGCTGATTACAAAGGTTGCC ACGTGCTGGAGAAGCAGGATGGGCGTTTCCACCTGAGGGTGTTCATACAAGCTGTACTACCCAATGGCCG TGTGGATACAGCACAAGATGTCACTCTGATCTGTCCCAAACCAGACCACATCTTGACTCCAGAGTCCTAC CTGGCACCACCCACCACACCTCAGCCTTTTATACCTCATACTTTTGCCCTTCACCCCATCTCTGGCCACA CCTTGGCTGGGTCTGGCCACACTGGTCTCACTACATTGTACCCAGAGACCCATCCAACTCCCGCTCCACC ATCCTCAGAACCTGGACCTGTTGGACCCACCGTGCCTCAGTCCCAGTGGGGCACCTTGGGGTCCTGGGAA TTGACTGAGCTTGATTCTATAGGTACCCATCTGCTCCAGGAGCGGTGTCAGGTAGCCTCTGGGCACATCC CCTGCATGGTAAAAGGTAGTTCCGAGGAAGCCTGTCAGCAGGCTGGCTGCTGCTATGACAATACCAAAGA AATGCCCTGTTACTATGGCAACACAGTCACTCTCCAGTGTTTCAGAAGTGGCTACTTTACCTTGGTCATG TCGCAAGAAACAGCCTTGACACACGGGGTCATGCTGGACAATGTCCACCTAGCCTATGCCCCCAACGGAT GCCCCCCTACCCAGAAGACAAGTGCTTTTGTGGTTTTCCATGTCCCTCTCACCCTCTGTGGAACAGCAAT CCAGGTGGTTGGTAAGCAGCTCGTCTATGAGAACCAGCTGGTGTCTAACATAGAGGTCCAAACGGGACCG CAGGGTTCCATCACTCGGGATGGTGTCTTCCGGCTTCATGTTCGCTGTATCTTCAATGCTAGTGATTTCC TGCCCATCCGGGCATCTATCTTCTCACCACAACCACCTGCCCCTGTGACCCGGTCTGGACCCCTGAGGCT GGAGCTGAGGATTGCCACGGATAAGACTTTCAGCTCCTACTACCAGGGGAGTGACTATCCCCTTGTGAGA CTGCTCCAGGAACCAGTTTACATAGAGGTCCGTCTCCTGCAGAGAACTGATCCTGGTCTGGCTCTCATGC TTCATCAGTGCTGGGCCACACCCTCTGCCAGCCCCTTTGAGCAGCCCCAATGGCCCATTCTGTCAGATGG GTGTCCTTTCAAGGGTGACAACTACAGAACACAAATGGTGGCTGCAGACAGGGCAACGCTGCCCTTCTGG TCTCACTATCAGCGCTTCACCATTGCCACCTTCACTCTCCTTGACTCCAGCTCCCAGAATGCCCTTCGGG GACAGGTCTATTTCTTCTGTAGTGCCTCTGCCTGCCACCCTGTGGGGTCCGAGACGTGCTCTACCACATG TGATTCTGAGATAGCAAGGCATCGACGATCCTCTGGTCACCACAATAGCACTATCCGGGCCCTGGACATT GTGAGCTCTCCAGGAGCAGTGGGCTTTGAGGATGCTCCTAAACTGGAGCCTTCAGGTTCCACCAGGAACT CCGGTTCAAGACCCTTACTCTGGGTGCTCCAACTGCTGGCCCTCACCCTAGTCCTGGGGGATGGTGTCTT GGTGGGTCTGAGCTGGGCCTGGGCCTGGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200920 representing NM_053509
Red=Cloning site Green=Tags(s) MAWGCFVVLLLLVAAPLRLGQHLHLKPGFQYSYDCGVQGMQLLVFPRPNQTIQFKVLDEFGNRFEVNNCS ICYHWVISEAQKPAVFSADYKGCHVLEKQDGRFHLRVFIQAVLPNGRVDTAQDVTLICPKPDHILTPESY LAPPTTPQPFIPHTFALHPISGHTLAGSGHTGLTTLYPETHPTPAPPSSEPGPVGPTVPQSQWGTLGSWE LTELDSIGTHLLQERCQVASGHIPCMVKGSSEEACQQAGCCYDNTKEMPCYYGNTVTLQCFRSGYFTLVM SQETALTHGVMLDNVHLAYAPNGCPPTQKTSAFVVFHVPLTLCGTAIQVVGKQLVYENQLVSNIEVQTGP QGSITRDGVFRLHVRCIFNASDFLPIRASIFSPQPPAPVTRSGPLRLELRIATDKTFSSYYQGSDYPLVR LLQEPVYIEVRLLQRTDPGLALMLHQCWATPSASPFEQPQWPILSDGCPFKGDNYRTQMVAADRATLPFW SHYQRFTIATFTLLDSSSQNALRGQVYFFCSASACHPVGSETCSTTCDSEIARHRRSSGHHNSTIRALDI VSSPGAVGFEDAPKLEPSGSTRNSGSRPLLWVLQLLALTLVLGDGVLVGLSWAWAWA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053509 |
ORF Size | 1851 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053509.1, NP_445961.1 |
RefSeq Size | 1947 bp |
RefSeq ORF | 1854 bp |
Locus ID | 85271 |
UniProt ID | O54766 |
Cytogenetics | 1q43 |
MW | 67.9 kDa |
Gene Summary | glycoprotein component of the zona pellucida [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200920 | Zp1 (untagged ORF) - Rat zona pellucida glycoprotein 1 (sperm receptor) (Zp1), (10 ug) |
USD 633.00 |
|
RR200920L3 | Lenti ORF clone of Zp1 (Myc-DDK-tagged ORF) - Rat zona pellucida glycoprotein 1 (sperm receptor) (Zp1), (10 ug) |
USD 932.00 |
|
RR200920L4 | Lenti ORF clone of Zp1 (mGFP-tagged ORF) - Rat zona pellucida glycoprotein 1 (sperm receptor) (Zp1), (10 ug) |
USD 932.00 |
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