Ppp1r13b (NM_001108062) Rat Tagged ORF Clone

CAT#: RR200854

Ppp1r13b (Myc-DDK-tagged ORF) - Rat protein phosphatase 1, regulatory (inhibitor) subunit 13B (Ppp1r13b), (10 ug)

Product Images

Circular map for RR200854

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp1r13b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200854 representing NM_001108062
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATATTAACCGTGTTCTTGAGCAACAATGAACAGATTTTAACAGAAGTTCCTATAACACCTGAAACCA
  CCTGTCGAGATGTCGTAGAATTCTGCAAGGAACCTGGAGAGGGCGGCTGCCACCTGGCCGAAGTGTGGAG
  AGGAAGTGAGCGTCCCATCCCCTGTGACCACATGATGTATGAGCACCTACAGAAGTGGGGGCCTCGCAGG
  GAAGAGGTAAAATTTTTTCTTCGCCATGAGGATTCTCCAACTGAGAGCAGTGAACAAGGTGCCCGTCAGA
  GCCAAGAGCAGAGAACGCAGAGAAACGTTATGAATGTTCCTGGAGAGAAGCACACTGAAAATGGGGAACA
  GCGTTTACATTTCCTAAAGCAACAGGAGCGCCGCCAGCAGCAGTCCATTTCTGAGAATGAGAAGCTTCAG
  AAACTGAAGGAACGAGTGGAAGCCCAGGAGAACAAGCTGAAGAAGATCCGGGCCATGAGAGGACAAGTGG
  ACTACAGCAAAATCATGAATGGCAATCTGTCTGCTGAGATTGAAAGGTTCAGCGCCATGTTCCAGGAAAA
  GAAGCAGGAAGTTCAGACTGCCATCTTACGAGTCGATCAGCTCAGTCAGCAACTAGAAGATTTAAAGAAA
  GGAAAGCTGAATGGGTTCCAGTCATACAACGGCAGGTTGACAGGGCCAGCAGCAGTGGAGTTAAAACGAC
  TCTACCAAGAGCTGCAGATTCGTAACCAGCTTAATCAGGAGCAGAATTCCAAGCTCCAGCAGCAGAAGGA
  GCTCCTGAACAAGCGCAACATGGAGGTGGCCATGATGGATAAGCGGATCAGCGAGCTTCGTGAGCGCCTT
  TACGGCAAGAAAATCCAGGCACCTGAACCGTGTGAATGGGACATCATCACCACAGTCACCCCTGAGCACA
  TCCGGCCGGGTCGCTGCTGTGGGTCCTTATATCCAGGGGATCCTGTAAAGCCCCAGTCTCTCACCATTGC
  CTCGAGTGCTGCCCACGGAAGATCCAAATCTGCTAATGATGGAAACTGGCCAACGTTAAAGCAGAATTCT
  GCTTCAGTGAAGTCAACTCAGATGGCTGGTGGGGACTGGAAGGACTCAGGCATGGAGGGGACCCTAAAGC
  AAGGTGCCATCTCAAGCCAGCCTTTGCCTCTGTCCGCACTGGGAGCTACAGACAAGCTGGGCATCGAGAT
  TGGCAAAGGGCCACCTCCCATACCTGGTGTAGGCAAGCCACTTCCCCCAAGTTATGGGACATATCCAAGC
  CCTGGGCCCCTGGGCCCAGGGTCAACAAGCTCCCTGGAGAGGAGGAAAGAAGGAAGCTTGCCCAGACCTG
  CTGCAGGCCCACCGAGTCGGCAGAAGCCCGCCCCACTGCCCCCAGCTGGCAGCGCTCCCCAACCCAGCTC
  CTCACAGCAGATTCAGCAGAGGATCTCAGTGCCACCAAGTCCCACATACCCACCAGCGGGGCCCCCTGCC
  TTCCCAGCTGGAGACGGAAAACCTGAGCTGCCACTCACAGTGGCCATTAGGCCTTTCCTGGCTGATAAAG
  GGTCAAGGCCACAGTCTCCTAGGAAGGGACCTCAGACAGTAAACTCAAGCTCTATATACTCCATGTACCT
  CCAGCAAGCCACGCCACCTAAGAATTACCAGCCACCAGCTCATGGCACCCTAAACAAGTCAGTTAAAGCA
  GTGTATGGTAAACCTGTCCTACCGTCAGGGTCAGCGTCTCCATCGCCCTTACCGTTTCTTCATGGGTCGC
  TGGGCACAGGCACGACACAGCCGCAGCCACCTTCAGAATGTGCTGAGAAGGAGGCAGAGCCGGAAGGCCC
  CAGCGTGCCTGGAGAAGGCAGCACGGTGGAAAGCCTGCCACGGCCGCTCAGTCCCACCAAACTCACACCC
  ATTGTACACTCCCCACTCCGCTACCAGAGCGATGCGGACCTGGAAGCTCTCCGCAGGAAGCTGGCCAATG
  CACCCCGACCCCTGAAGAAGCGCAGCTCTATCACGGAGCCAGAGGGCCCTGGAGGGCCCAACATTCAAAA
  GTTGCTTTATCAACGCTTCAACACCCTGGCTGGCGGCATGGAGGGTACCCCTTTCTACCAGCCCAGCCCG
  TCACAGGACTTTGTGGGCACCTTAGCTGATGTGGACAATGGAAACACCAATGCCAATGGCAACTTGGATG
  AGTCTTTCCCTCCCCGGCCCACGGCCCCACTTCCTGATGAGCTTGCCCCATCCTCAGATGCCAATGACAA
  TGAGTTACCTTCCCCTGAGCCAGAGGAGCTCATTTGTCCCCAGACAACTCATCAAACTGCTGAGCCCACT
  GAGGACAATAACAACAACGTGGCCACGGTACCCTCCACAGAGCAGATCCCAAGTCCTGTGGCTGAGGCTC
  CTTCCGAGGAGGATCAGGTCCCTCCTGCACCTCTTTCCCCTGTCGTCCATCCTCCAACAGCGTCCGCGAG
  CAAGCGGACCAACCTGAAGAAGCCTAACTCTGAGCGGACAGGGCATGGGCTGAGAGTGCGCTTCAACCCC
  CTGGCACTGCTTCTAGATGCTTCTCTGGAAGGAGAGTTTGATCTAGTGCAGAGGATCATCTATGAGGTGG
  AAGACCCTAGCAAACCCAATGACGAAGGGATCACCCCCCTACACAATGCTGTCTGTGCTGGCCACCACCA
  TATCGTGAAGTTTCTGCTGGACTTTGGTGTCAATGTGAATGCTGCAGACAGTGATGGATGGACACCACTG
  CACTGTGCCGCCTCTTGCAACAGTGTCCACCTCTGCAAGCAGCTGGTGGAGAGTGGAGCCGCTATCTTTG
  CCTCCACCATCAGTGACATCGAAACCGCTGCAGACAAGTGTGAGGAGATGGAGGAGGGCTATATCCAGTG
  TTCTCAGTTCCTGTACGGGGTACAAGAGAAGCTGGGGGTCATGAACAAAGGCATTGTGTATGCTCTGTGG
  GACTACGAAGCCCAGAACAATGATGAGCTGTCCTTCCATGAAGGGGATGCCATCACCATCCTGAGGCGCA
  AAGACGAAAACGAGACCGAGTGGTGGTGGGCTCGTCTTGGGGACCGGGAGGGCTATGTGCCCAAAAACTT
  GCTTGGGTTGTACCCACGGATCAAGCCCCGGCAGCGGACACTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200854 representing NM_001108062
  Red=Cloning site Green=Tags(s)
  
  MILTVFLSNNEQILTEVPITPETTCRDVVEFCKEPGEGGCHLAEVWRGSERPIPCDHMMYEHLQKWGPRR
  EEVKFFLRHEDSPTESSEQGARQSQEQRTQRNVMNVPGEKHTENGEQRLHFLKQQERRQQQSISENEKLQ
  KLKERVEAQENKLKKIRAMRGQVDYSKIMNGNLSAEIERFSAMFQEKKQEVQTAILRVDQLSQQLEDLKK
  GKLNGFQSYNGRLTGPAAVELKRLYQELQIRNQLNQEQNSKLQQQKELLNKRNMEVAMMDKRISELRERL
  YGKKIQAPEPCEWDIITTVTPEHIRPGRCCGSLYPGDPVKPQSLTIASSAAHGRSKSANDGNWPTLKQNS
  ASVKSTQMAGGDWKDSGMEGTLKQGAISSQPLPLSALGATDKLGIEIGKGPPPIPGVGKPLPPSYGTYPS
  PGPLGPGSTSSLERRKEGSLPRPAAGPPSRQKPAPLPPAGSAPQPSSSQQIQQRISVPPSPTYPPAGPPA
  FPAGDGKPELPLTVAIRPFLADKGSRPQSPRKGPQTVNSSSIYSMYLQQATPPKNYQPPAHGTLNKSVKA
  VYGKPVLPSGSASPSPLPFLHGSLGTGTTQPQPPSECAEKEAEPEGPSVPGEGSTVESLPRPLSPTKLTP
  IVHSPLRYQSDADLEALRRKLANAPRPLKKRSSITEPEGPGGPNIQKLLYQRFNTLAGGMEGTPFYQPSP
  SQDFVGTLADVDNGNTNANGNLDESFPPRPTAPLPDELAPSSDANDNELPSPEPEELICPQTTHQTAEPT
  EDNNNNVATVPSTEQIPSPVAEAPSEEDQVPPAPLSPVVHPPTASASKRTNLKKPNSERTGHGLRVRFNP
  LALLLDASLEGEFDLVQRIIYEVEDPSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPL
  HCAASCNSVHLCKQLVESGAAIFASTISDIETAADKCEEMEEGYIQCSQFLYGVQEKLGVMNKGIVYALW
  DYEAQNNDELSFHEGDAITILRRKDENETEWWWARLGDREGYVPKNLLGLYPRIKPRQRTLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108062
• ORF Size: 3126 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108062.1, NP_001101532.1
• RefSeq Size: 4187 bp
• RefSeq ORF: 3129 bp
• Locus ID: 314465
• Cytogenetics: 6q32
• MW: 114.3 kDa