Epn2 (NM_021852) Rat Tagged ORF Clone

CAT#: RR200322

Epn2 (Myc-DDK-tagged ORF) - Rat epsin 2 (Epn2), transcript variant 1, (10 ug)

Product Images

Circular map for RR200322

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Epn2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200322 representing NM_021852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACTTCATCTATTAGACGGCAGATGAAAAACATTGTGAACAATTACTCAGAGGCTGAAATCAAAG
  TCCGGGAAGCCACCTCCAATGACCCATGGGGCCCATCCAGCTCTCTGATGACTGAGATTGCTGACCTGAC
  CTATAATGTGGTAGCCTTCTCGGAGATCATGAGCATGGTTTGGAAGCGGCTTAATGACCATGGCAAGAAC
  TGGCGACATGTATACAAGGCGCTGACACTGCTGGACTACCTTATCAAGACAGGTTCTGAGCGGGTGGCCC
  AGCAGTGTCGGGAGAACATCTTTGCTATACAGACTCTGAAGGACTTCCAGTACATTGACCGTGATGGCAA
  GGACCAGGGTATTAATGTTCGAGAGAAGTCAAAGCAACTGGTTGCTCTCCTCAAGGATGAGGAGCGGCTG
  AAGGTTGAGAGGGTTCAGGCTCTCAAAACCAAAGAGCGCATGGCTCAGGTGGCCACTGGTGTGGGCAGCA
  ACCAGATCACCTTCGGTCGAGGCTCCAGCCAGCCCAACCTTTCTACCAGCTACTCAGAGCAAGAGTATGG
  CAAGGCTGGGGGCTCGCCGGCGTCCTACCACGGCTCTACTTCCCCACGAGTGTCCTCTGAGTTGGAGCAG
  GCCCGGCCACAGACCAGCGGAGAAGAGGAGCTGCAGCTGCAACTGGCACTTGCCATGAGCAGAGAGGTTG
  CAGAACAGGAAGAACGCCTCAGGCGGGGTGATGACCTCAGGTTGCAGATGGCTCTGGAAGAAAGCCGGAG
  AGACACAGTAAAAGTTCCAAAAAAGAAAGAGGTGAAAGCTTGCTGCAAGCCAGGCTCCCACTCGCAGCAG
  ACTACCTTGTTGGATTTAATGGATGCCCTCCCCAGCTCAGGCCCTGTTGCACAGAAAACTGAGCCGTGGA
  GTACGGGAACCCCTGCCAACCAGACCAACCCCTGGGGTGGAACCGTGGCACCTGCGAACATTTCTGACCC
  CTGGCCTTCATTTGGTACCAAGCCAGCTGCCTCTGTGGACCCCTGGGGAGTACCTACCACAGCCAGCATA
  CAGTCTGTCCCCAAGAACTCAGACCCTTGGGCAGCCTCACAGCAGCCTGCCTCCGATGCTGGAAAAACAG
  CTGATGCCTGGGGGGCTGCCAAGCCTAGTCCTGCCTCAGGGTCCTTTGAGCTCTTCAGTAATTTCAACGG
  TACAGTTAAAGACGATTTTTCTGAATTCGACAACCTTCGAACTTCAAAAAAACCAGCTGAGTCAGGGGCC
  TCAGTACCACCCCAGGACAGCAGAACCACGAGCCCTGACCTCTTTGAGTCTCAATCCTTGACTTCTGCCT
  CGAGCAAGCCTAGCAGTGCTCGGAAAACACCTGAGTCCTTCCTGGGCCCCAATGCAGCACTGGTGAACCT
  GGACTCACTGGTGACTAAGCCTAATCCACCAGCTCAGTCCCTCAATCCCTTCCTGGCACCAGGTGCTGCT
  GCTCCAGCTCCTGTCAATCCCTTTCAGGTCAACCAGCCCCAGCCACTGACACTGAACCAGCTTCGGGGAA
  GCCCTGTCCTGGGAAGCAGTGCGTCCTTTGGGTCTGGTCCAGGGGTGGAGACGGTGGCTCCCATGCCCTC
  TGTAGCTCCACACTCAGCACTGGGGGCCACTGGCTCCTCATTGACACCACTAGGCCCTACAGCAATGAAC
  ATGGTAGGCAGTGTGGGTATTCCCCCATCAGCAGCTCAGCCAGCGGGCACAACCAACCCTTTCCTTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200322 representing NM_021852
  Red=Cloning site Green=Tags(s)
  
  MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKN
  WRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERL
  KVERVQALKTKERMAQVATGVGSNQITFGRGSSQPNLSTSYSEQEYGKAGGSPASYHGSTSPRVSSELEQ
  ARPQTSGEEELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKVPKKKEVKACCKPGSHSQQ
  TTLLDLMDALPSSGPVAQKTEPWSTGTPANQTNPWGGTVAPANISDPWPSFGTKPAASVDPWGVPTTASI
  QSVPKNSDPWAASQQPASDAGKTADAWGAAKPSPASGSFELFSNFNGTVKDDFSEFDNLRTSKKPAESGA
  SVPPQDSRTTSPDLFESQSLTSASSKPSSARKTPESFLGPNAALVNLDSLVTKPNPPAQSLNPFLAPGAA
  APAPVNPFQVNQPQPLTLNQLRGSPVLGSSASFGSGPGVETVAPMPSVAPHSALGATGSSLTPLGPTAMN
  MVGSVGIPPSAAQPAGTTNPFLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021852
• ORF Size: 1749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021852.3, NP_068624.2
• RefSeq Size: 4053 bp
• RefSeq ORF: 1752 bp
• Locus ID: 60443
• Cytogenetics: 10q22
• MW: 62.3 kDa
• Gene Summary: The protein encoded by this gene is a member of the epsin protein family. Epsin proteins are endocytic adaptors that function in the formation of clatherin-coated vesicles. Epsins contain a highly conserved N-terminal homology domain that binds phosphatidylinositol 4,5-bisphosphate in the plasma membrane, two or three ubiquitin interacting motifs, two clathrin-binding motifs, a cluster of aspartate-proline-tryptophan/phenylalanine repeats, and two or three asparagine-proline-phenylalanine tripeptide repeats at the C-terminus. In mouse, simultaneous knockout of this gene and its paralog results in embryonic arrest due to disruption of Notch signaling, suggesting a role as a specialized endocytic adaptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]