Araf (NM_022532) Rat Tagged ORF Clone
CAT#: RR200318
- TrueORF®
Araf (Myc-DDK-tagged ORF) - Rat v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 1, (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_022532" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Araf |
Synonyms | Araf1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200318 representing NM_022532
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCACCACGAGGCCCTCCTGCTAGTGGGGCTGAGCCATCTCGGGCAGTTGGCACTGTCAAAGTGT ACCTGCCTAACAAGCAACGCACAGTGGTGACTGTCCGGGATGGCATGAGTGTCTATGACTCTTTGGACAA GGCCCTCAAGGTGCGGGGTCTCAATCAGGACTGCTGTGTGGTCTACAGACTCATCAAAGGAAGAAAGACA GTCACTGCCTGGGACACAGCCATTGCACCTCTGGATGGCGAGGAGCTCATTGTGGAGGTCCTGGAAGATG TGCCACTGACCATGCACAATTTTGTACGGAAGACATTCTTCAGTTTGGCCTTCTGTGACTTCTGCCTTAA GTTTCTGTTCCACGGCTTTCGCTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC CCCACGGTCTGCGTTGACATGAGTACCAACCGCCGACAGTTCTACCACAGCATCCAGGATTTGTCTGGAG GCTCCAGGCAGCAGGAGGTTCCCTCAAATCTCTCTGTGAATGAGCTGCTAACCCCCCAGGGTCCCAGTCC CTTTACCCAGCAACGTGACCAGGAGCACTTCTCCTTCCCTGCCCCTGCCAATCCCCCACTGCAGCGCATC CGCTCCACATCTACTCCTAACGTCCACATGGTCAGCACCACAGCTCCCATGGACTCCAGCCTCATGCAGT TTACTGCTCAGAGCTTCAGCACCGATGCTGCTGGTAGAGGTGGTGATGGAGCTCCTCGGGGTAGCCCTAG CCCAGCTAGTGTGTCTTCAGGGAGGAAGTCCCCACATTCCAAGTTACCTGCAGAACAGCGGGAACGGAAG TCCTTGGCAGATGAAAAGAAAAAAGTGAAGAACCTGGGGTACCGGGACTCAGGCTATTACTGGGAGGTGC CACCCAGTGAGGTACAGCTGTTGAAGAGGATCGGGACAGGCTCTTTTGGCACTGTGTTTCGGGGGCGTTG GCATGGCGATGTAGCTGTGAAAGTGCTCAAGGTGGCCCAACCTACCGCTGAGCAGGCCCAGGCCTTCAAG AATGAGATGCAGGTGCTCAGGAAGACACGACATGTCAACATTTTGCTGTTTATGGGTTTCATGACTCGGC CAGGGTTTGCCATCATCACACAGTGGTGTGAGGGTTCCAGCCTCTACCACCACCTACATGTGGCTGACAC GCGCTTTGACATGGTCCAGCTCATTGATGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTCCACGCC AAGAACATCATTCACCGAGACCTAAAGTCCAACAATATCTTCCTACATGAGGGGCTCACAGTCAAGATTG GTGACTTCGGCCTGGCCACAGTGAAGACACGATGGAGTGGGGCCCAGCCCTTAGAGCAGCCCTCAGGGTC TGTGCTGTGGATGGCAGCTGAGGTGATCCGAATGCAGGACCCGAACCCCTACAGCTTCCAGTCGGATGTC TATGCCTATGGTGTTGTGCTCTATGAGCTTATGACCGGCTCACTGCCCTACAGCCACATTGGCAGCCGTG ACCAGATCATCTTTATGGTGGGTCGTGGCTATCTGTCTCCGGACCTCAGCAAAATCTTCAGTAATTGCCC CAAAGCCATGAGGCGCTTGCTGACTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCTCTATTTCCCCAG ATTCTGGCCACGATCGAGCTGCTGCAGCGGTCACTCCCCAAGATTGAGCGGAGTGCCTCCGAACCCTCCT TGCACCGTACCCAGGCTGATGAGTTGCCTGCCTGCCTTCTCAGCGCAGCCCGCCTTGTGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200318 representing NM_022532
Red=Cloning site Green=Tags(s) MEPPRGPPASGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKT VTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKV PTVCVDMSTNRRQFYHSIQDLSGGSRQQEVPSNLSVNELLTPQGPSPFTQQRDQEHFSFPAPANPPLQRI RSTSTPNVHMVSTTAPMDSSLMQFTAQSFSTDAAGRGGDGAPRGSPSPASVSSGRKSPHSKLPAEQRERK SLADEKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVAQPTAEQAQAFK NEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHA KNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDV YAYGVVLYELMTGSLPYSHIGSRDQIIFMVGRGYLSPDLSKIFSNCPKAMRRLLTDCLKFQREERPLFPQ ILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022532 |
ORF Size | 1812 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022532.2, NP_071977.1 |
RefSeq Size | 2350 bp |
RefSeq ORF | 1815 bp |
Locus ID | 64363 |
UniProt ID | P14056 |
Cytogenetics | Xq11 |
MW | 67.6 kDa |
Gene Summary | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200318 | Araf (untagged ORF) - Rat v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 1, (10 ug) |
USD 619.00 |
|
RR200318L3 | Lenti ORF clone of Araf (Myc-DDK-tagged ORF) - Rat v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 1, (10 ug) |
USD 918.00 |
|
RR200318L4 | Lenti ORF clone of Araf (mGFP-tagged ORF) - Rat v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 1, (10 ug) |
USD 918.00 |
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