Ndufv3 (NM_022607) Rat Tagged ORF Clone

CAT#: RR200315

Ndufv3 (Myc-DDK-tagged ORF) - Rat NADH dehydrogenase (ubiquinone) flavoprotein 3 (Ndufv3), nuclear gene encoding mitochondrial protein, transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ndufv3
• Synonyms: Mipp65; Ndufv3l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200315 representing NM_022607
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCTCTCTCCTGCTGCGGGGAGGACGAATCCGGGCGCTGAAGGCTGCCCTTCTCGAGGCAAAGG
  TGTTCCGAGGAGAACTGGCTTCTACAGTGCCCCTGTCTACAGAGTCCGAGAATAATAAAAAGGCGGCAGG
  ACCAACTAGCAAGACGGAAAGTGTGTTCAAAGATGTGGTGGGAGCAGAGGAGAGGGCCAAGCTCCTAGAC
  ACTCACACAGCAGCGGCACTGTCCAAAAGCTCCCCTCCACCCAGTTCTTACCCATCAGTGGAGAACACAG
  GTGGGGCTGTCGCAGGCCTGCCGCTCACAGACTTGTCAAGAAAGACTTTGGTAGCGTTTCCTCAGAAAGT
  TACGCCTCCACCGCATGCCTGGGCTTCTGACTCGGAGGCTCGGCGGCATGTCCAGAAAGTGACGAAAGAT
  TCATCTTCATCTTCCTCATCCAGCTCCTCAGATTCAGACTCTGATGGGGAGGAACATGGCTCCGACATTG
  GTCCCCGAGTGGCAAGCAAAAGCAAGGCAGGGTTTTCCAAACCTGAGGCTTCTCGTCCCTCGAAGAACGG
  AGCCCCTAAGATCACAGTTTTTGCAAAAGAGAAGGCCAAGGTGCAGAAGCCACACACAGATGTCACCTAC
  CCAGAGAAGCCCCTGCAGCCTAAGAAGAAAGGGACTTTCACCAAGCCTGTAGAAGATAGTAAAGAAACCA
  GGTCCAAGCTCATGACATCCAAGCCACAGTCCAGTGAGGTTTTGGAGCAAAAAATGAAGGAAAAACAGCA
  CCAGGGAAAACGGAGACCAGACAAGACAGGAAAGGAAAGCACAGAGCTGTTTGAAGCTGAAGGAATTTTA
  CCCGGCCACAGGAAAGCCAGAGTTTCCACACAGCCCACAACCGGAACACAAGAGGCCAGCGCAGAACTCC
  GGCCAGCCGCTGCCCCCGAGTCGGGAGCTAGACAGGAAGCCAAAGGGCCAGAGCTCGAATGGAAGACCGC
  TTCTCCCCTGGTCAGAAAAGAAAGCCTGGAGAAGCAGGTGCCAGAAGGGAGCTTCCAGGCGGAGGAGGAG
  ACCTCAGGAGATCAGACACTAGGGAGATATTCGAAGACAGTTCCTGTTGAGGAGAAAAATACCTTTGGAG
  AGAGAGCTGGACCACAACTGGAGGGGACGTTCCAGGCGCCAGCCGGCGAAGCCCCGCCCACTGATGCAGG
  CCCACCCCAGGAAGCCCCAGGCGACACACAGGAGCCCACACTGGTTCCCGAGTCGAGTGACACCACCACC
  TACAAGAACCTGCAGCATCATGAGTACAACGCATTCACCTTCCTAGACCTGAACCTGGACCTCTCCAAGT
  TCAGGCTGCCACAGCCGTCTTCGGGACGAGAGTCGCCCCGGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200315 representing NM_022607
  Red=Cloning site Green=Tags(s)
  
  MAVSLLLRGGRIRALKAALLEAKVFRGELASTVPLSTESENNKKAAGPTSKTESVFKDVVGAEERAKLLD
  THTAAALSKSSPPPSSYPSVENTGGAVAGLPLTDLSRKTLVAFPQKVTPPPHAWASDSEARRHVQKVTKD
  SSSSSSSSSSDSDSDGEEHGSDIGPRVASKSKAGFSKPEASRPSKNGAPKITVFAKEKAKVQKPHTDVTY
  PEKPLQPKKKGTFTKPVEDSKETRSKLMTSKPQSSEVLEQKMKEKQHQGKRRPDKTGKESTELFEAEGIL
  PGHRKARVSTQPTTGTQEASAELRPAAAPESGARQEAKGPELEWKTASPLVRKESLEKQVPEGSFQAEEE
  TSGDQTLGRYSKTVPVEEKNTFGERAGPQLEGTFQAPAGEAPPTDAGPPQEAPGDTQEPTLVPESSDTTT
  YKNLQHHEYNAFTFLDLNLDLSKFRLPQPSSGRESPRH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_022607
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022607.2, NP_072129.2
• RefSeq Size: 1536 bp
• RefSeq ORF: 1377 bp
• Locus ID: 64539
• Cytogenetics: 20p12
• MW: 49.3 kDa
• Gene Summary: heat stable phosphoprotein localized to the mitochondrion; may undergo arachidonic acid dependent phosphorylation by PKN [RGD, Feb 2006]