Grik3 (NM_181373) Rat Tagged ORF Clone

CAT#: RR200120

Grik3 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 3 (Grik3), transcript variant 2, (10 ug)

Product Images

Circular map for RR200120

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grik3
• Synonyms: GluK3; gluR-7; GluR7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200120 representing NM_181373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGCTCCCTGGCGGCGCCTCCGGAGTCTGGTTTGGGAATACTGGGCCGGGTTCCTCGTGTGCGCCT
  TCTGGATCCCAGACTCGCGCGGGATGCCCCACGTCATCCGGATCGGCGGAATCTTTGAGTACGCGGACGG
  CCCCAACGCCCAGGTCATGAACGCTGAGGAGCACGCCTTTCGGTTTTCTGCCAATATCATCAACAGGAAC
  AGAACTCTGCTGCCCAACACGACCCTGACTTACGACATTCAGAGGATTCACTTCCATGACAGTTTTGAGG
  CCACCAAGAAGGCCTGTGACCAGTTGGCGCTCGGTGTGGTAGCCATCTTTGGGCCATCCCAGGGCTCCTG
  CACCAATGCCGTCCAGTCCATCTGCAATGCCTTGGAGGTTCCTCACATCCAACTGCGCTGGAAGCACCAC
  CCCCTGGACAACAAGGACACCTTCTACGTGAACCTCTACCCCGACTACGCCTCTCTCAGCCACGCCATCC
  TCGACTTGGTCCAGTCCCTCAAGTGGCGGTCAGCCACCGTAGTCTATGATGACAGTACAGGTCTCATCCG
  GCTGCAGGAGCTCATCATGGCTCCATCTAGGTACAACATCCGCCTGAAGATTCGCCAGCTCCCCATCGAC
  TCCGATGACTCACGCCCCTTGCTCAAAGAGATGAAGCGGGGCCGGGAGTTCCGTATCATCTTTGACTGCA
  GTCACACCATGGCAGCCCAGATCCTCAAGCAGGCCATGGCCATGGGCATGATGACGGAATACTACCACTT
  CATCTTCACCACTCTGGATCTCTATGCGCTAGACCTGGAACCCTACCGCTACTCGGGAGTGAACCTGACT
  GGGTTCCGCATACTCAACGTGGACAACCCCCATGTCTCAGCCATTGTGGAGAAGTGGTCCATGGAGCGGC
  TACAGGCAGCTCCCCGGGCAGAGTCAGGCCTGCTGGATGGAGTGATGATGACCGATGCAGCCCTGCTCTA
  CGATGCGGTCCACATTGTGTCTGTGTGCTACCAGCGAGCGCCGCAGATGACTGTGAACTCCCTACAGTGC
  CATCGGCACAAGGCCTGGCGCTTCGGTGGCCGCTTCATGAACTTCATCAAGGAGGCTCAATGGGAAGGAT
  TAACTGGACGGATTGTTTTCAACAAAACTAGTGGCTTGCGGACTGATTTTGATCTGGACATCATCAGCCT
  CAAGGAAGATGGCCTCGAGAAGGTCGGGGTGTGGAGTCCAGCTGACGGTCTCAATATCACTGAGGTTGCC
  AAAGGCCGAGGTCCTAATGTCACCGACTCTCTGACCAACAGGTCACTCATCGTCACCACTCTGCTGGAGG
  AGCCTTTTGTCATGTTCCGCAAGTCTGATAGGACCCTTTACGGCAATGACCGGTTCGAGGGCTACTGCAT
  CGACTTGCTCAAGGAGCTGGCGCACATCCTGGGCTTCTCCTACGAGATCCGGCTGGTGGAAGACGGCAAG
  TACGGGGCACAGGACGACAAGGGCCAGTGGAACGGCATGGTCAAGGAACTCATTGACCACAAAGCAGATC
  TGGCTGTGGCTCCCCTGACCATCACCCATGTCCGAGAGAAGGCCATTGACTTCTCTAAGCCTTTTATGAC
  CCTCGGAGTGAGCATCTTATATCGAAAACCCAATGGCACCAACCCCAGTGTCTTCTCCTTCCTCAACCCC
  CTGTCCCCAGACATCTGGATGTACGTGCTACTCGCCTACCTGGGTGTCAGCTGTGTCCTCTTCGTCATTG
  CCAGATTCAGCCCTTATGAATGGTATGATGCCCACCCCTGCAACCCCGGCTCTGAGGTGGTGGAGAATAA
  CTTCACGCTGCTCAACAGCTTCTGGTTTGGAATGGGCTCCCTGATGCAACAAGGATCTGAACTGATGCCC
  AAAGCTCTGTCTACCCGCATCATTGGCGGCATCTGGTGGTTCTTCACCCTTATTATCATCTCCTCCTACA
  CGGCCAACCTGGCTGCCTTCCTGACCGTGGAGCGCATGGAGTCACCCATCGACTCTGCCGATGACCTGGC
  CAAGCAGACCAAAATAGAGTACGGTGCTGTCAAGGATGGGGCCACCATGACCTTCTTCAAGAAATCCAAG
  ATCTCCACCTTTGAGAAGATGTGGGCCTTCATGAGCAGCAAGCCCTCGGCTCTGGTGAAGAACAATGAGG
  AGGGCATCCAGCGGACACTCACAGCTGACTACGCTCTGCTCATGGAGTCCACGACCATAGAGTACATCAC
  ACAAAGGAACTGCAATCTCACCCAGATCGGCGGCCTCATCGATTCCAAGGGCTACGGCATCGGCACGCCC
  ATGGGCTCCCCCTACAGGGACAAAATCACCATCGCCATTCTGCAGCTGCAGGAGGAGGACAAGCTGCACA
  TCATGAAGGAGAAGTGGTGGCGAGGCAGCGGGTGCCCCGAGGAGGAGAACAAGGAGGCCAGCGCACTGGG
  CATCCAGAAGATTGGCGGCATCTTCATCGTCCTGGCTGCCGGCTTAGTCCTGTCCGTGTTGGTGGCAGTG
  GGCGAGTTTATATACAAACTCCGCAAGACAGCGGAACGGGAGCAGGTGAGGCCCTGGAGGAGGCTGCGTT
  GGACAGGGAAGGAAGCGCTCTTTCTGCAGCACAGTGGCCGACGAGATCCGCTTCTCCCTCACCTGCCAGC
  GGCGTCTCAAGCACAAGCCACAGCCTCCTATGATGGTCAAGACAGATGCGGTTATCAACATGCACACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200120 representing NM_181373
  Red=Cloning site Green=Tags(s)
  
  MTAPWRRLRSLVWEYWAGFLVCAFWIPDSRGMPHVIRIGGIFEYADGPNAQVMNAEEHAFRFSANIINRN
  RTLLPNTTLTYDIQRIHFHDSFEATKKACDQLALGVVAIFGPSQGSCTNAVQSICNALEVPHIQLRWKHH
  PLDNKDTFYVNLYPDYASLSHAILDLVQSLKWRSATVVYDDSTGLIRLQELIMAPSRYNIRLKIRQLPID
  SDDSRPLLKEMKRGREFRIIFDCSHTMAAQILKQAMAMGMMTEYYHFIFTTLDLYALDLEPYRYSGVNLT
  GFRILNVDNPHVSAIVEKWSMERLQAAPRAESGLLDGVMMTDAALLYDAVHIVSVCYQRAPQMTVNSLQC
  HRHKAWRFGGRFMNFIKEAQWEGLTGRIVFNKTSGLRTDFDLDIISLKEDGLEKVGVWSPADGLNITEVA
  KGRGPNVTDSLTNRSLIVTTLLEEPFVMFRKSDRTLYGNDRFEGYCIDLLKELAHILGFSYEIRLVEDGK
  YGAQDDKGQWNGMVKELIDHKADLAVAPLTITHVREKAIDFSKPFMTLGVSILYRKPNGTNPSVFSFLNP
  LSPDIWMYVLLAYLGVSCVLFVIARFSPYEWYDAHPCNPGSEVVENNFTLLNSFWFGMGSLMQQGSELMP
  KALSTRIIGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVKDGATMTFFKKSK
  ISTFEKMWAFMSSKPSALVKNNEEGIQRTLTADYALLMESTTIEYITQRNCNLTQIGGLIDSKGYGIGTP
  MGSPYRDKITIAILQLQEEDKLHIMKEKWWRGSGCPEEENKEASALGIQKIGGIFIVLAAGLVLSVLVAV
  GEFIYKLRKTAEREQVRPWRRLRWTGKEALFLQHSGRRDPLLPHLPAASQAQATASYDGQDRCGYQHAHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181373
• ORF Size: 2730 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181373.3, NP_852038.2
• RefSeq Size: 3634 bp
• RefSeq ORF: 2733 bp
• Locus ID: 298521
• UniProt ID: P42264
• Cytogenetics: 5q36
• MW: 103.1 kDa
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Compared to other kainate or AMPA receptors, this subunit exhibits a lower sensitivity to glutamate, and thus may play a unique role in neurotransmission in the brain. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]