Thsd7a (NM_001191970) Rat Untagged Clone

CAT#: RN217676

Thsd7a (untagged) - Rat thrombospondin, type I, domain containing 7A (Thsd7a)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Thsd7a
• Synonyms: RGD1566201
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217676 representing NM_001191970
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCGAGCCGGGCGCCTGGCGTCCCTGAGCCGGGGCGTCCTGCAGCTGATGCGGCTGCCACTGC
  TCCTGCTACTCCTGCTGAGCTCTGGAGCCCGCGGGGCTGCGGCGCAGGGAGACACAGAGGTGCCCACCCT
  CTATCTGTGGAAGACGGGTCCCTGGGGACGGTGTATGGGAGATGACTGTGGACCGGGCGGCATCCAGACT
  CGGGCTGTGTGGTGCGCTCATTCGGAAGGCTGGACAACGTTACATACGAACTGCAAGCAGGCTGTGAGAC
  CCAGTAACCAGCAAAACTGCTTCAAAGTGTGTGACTGGCACAAAGAGTTGTACGACTGGAGGCTGGGACC
  CTGGAATCAGTGTCAGCCTGTGATTTCAAAAAGCCTCGAGAAAGCACGAGAATGTGTGAAGGGGGAGGAA
  GGCATCCAGGTGAGAGAGATAACGTGCATCCAGAAGGACAAGGACATTCCCGCAGAGGACATCATCTGCG
  AGTACTTTGAACCCAAGCCCCTCCTGGAACAGGCCTGCCTCATCCCTTGTCAGCAAGACTGCATCGTGTC
  TGAGTTCTCGTCCTGGTCTGAGTGCTCCAGGACCTGTGGCAGCGGCCTGCAGCACCGCACTAGGCACGTG
  GTCGCACCACCACAGTTTGGAGGCTCTGGCTGTCCCAACCTCACTGAGTTCCAGGTATGCCAGTCCAACC
  CCTGTGAGGCAGAGGAGAGTATGTATAGCTTGCAGGTAGGGCCCTGGAGCGCATGCTCAGTACCACACTC
  ACGTCAAGCCAGGCAAGCCAGGCGTCGTGGCAAAAACAAGGAGAGAGAGAAGGAGAGAGGAAAAGCTGTG
  AAGGATCCAGAGGCCCGTGAGCTCATCAAGAAGAAGAGAAACAGAAACAGACAAAACAGACAGGAGAACA
  GATACTGGGACATCCAGATTGGGTACCAGACCAGGGACGTGATGTGCTTAAACAGGACTGGGAAGTCTGC
  GGACCTCAGCTTTTGCCAACAAGAGAGACTGCCCATGACCTTCCAGTCCTGTGTGATCACCAAAGAGTGC
  CAGGTGTCTGAGTGGTCGGAGTGGAGCCCGTGTTCAAAAACATGCCACGATGTCACATCTCCTACAGGCA
  CTCGAGTAAGGACAAGGACCATCAGGCAGTTTCCAATTGGAAGTGAGAAGGAATGTCCGGAACTTGAGGA
  GAAAGAGCCCTGTTTGTCTCAAGGAGATGGAGCTGTCCTCTGTGCTACGTATGGCTGGAGAACTACAGAA
  TGGACAGAGTGCCACGTGGACCCTTTGCTTAGTCAGCAGGACAAGAGGCGTGCCAACCAGACAGCCCTCT
  GTGGGGGTGGTGTCCAGACCCGGGAAATATACTGCATACAGACCAACGACATCTTGCTCTCCCATATAAA
  TACACAGAAGGACAAAGAAGCTTCAAAACCAGTGGACTCAAAATTATGCACTGGTCCTGTTCCTAACACC
  ACACAGTTGTGCCACGTTCCTTGTCCAATTGAATGTGAGGTTTCACCTTGGTCTGCCTGGGGACCATGTA
  CTTATGAAAATTGTAATGACCAGCAAGGCAAAAAAGGATTCAAATTAAGGAAGCGGCGCATTACTAATGA
  GCCTACTGGAGGTTCTGGGGCAACTGGAAACTGCCCTCACTTATTGGAAGCCATTCCCTGTGAAGAGCCA
  TCCTGCTATGACTGGAAATCGGTGAGACTCGGAGACTGTGAACCAGATAATGGAAAAGCCTGTGGGCCCG
  GCACTCAAGTTCAAGAGGTTGTATGCATCAACAGTGATGGAGAAGAAGTGGACAGACAGCTATGCCGAGA
  TGCAATCTTTCCTATCCCTGTGGCCTGTGATGCCCCATGCCCGAAGGACTGTGTGCTCAGTGCATGGTCC
  ACGTGGTCCTCCTGTTCACACACCTGCTCTGGGAAAACCACAGAAGGAAAACAGATCCGAGCTCGGTCCA
  TTCTGGCCTATGCAGGAGAAGAAGGTGGAATTCGCTGCCCTAACAGCAGTGCTTTGCAAGAGGTGCGCAG
  CTGCAATGAGCATCCTTGCACTGTGTACCACTGGCAAACTGGTCCCTGGGGTCAGTGTATAGAGGACACA
  TCAGTGTCATCCTTCAATACAACCACAACATGGACTGGGGAGACGTCATGTTCTGTGGGCATGCAGACCC
  GGAAAGTCATCTGCGTGCGAGTCAACGTGGGCCAAGTGGGACCCAAGAAGTGTCCTGAAAGCCTTCGGCC
  TGAAACAGTGAGACCTTGCTTGCTTCCTTGTAGGAAGGATTGTATTATGACCCCATACAGTGACTGGACA
  CCATGTCCCTCTTCATGCAGAGAAGGGGACTCTGGAGCCAGGAAGCAGTCTCGACAGCGCGTCATCATTC
  AGCTGCCAGCCTATGGAGGCAGGGAATGCTCAGACCCACTCTATGAAGAAAAGGCCTGCGAGGCCCCTCC
  CACATGTCACAGCTACAGGTGGAAGACACACAAATGGCGCCGGTGCCAGTTAGTCCCTTGGAGCATTCAA
  CAGGATGTCCCTGGAGCACAGGAAGGCTGCGGGCCTGGACGGCAGGCAAGAGCCATTACTTGTCGAAAGC
  AAGATGGAGGGCAGGCTAGCATCCAGGAGTGCCTCCAGTATGCAGGCCCTGTGCCAGCCCTGACCCAGGC
  TTGCCAGATCCCCTGCCAAGATGACTGTCAATTTACCAGCTGGTCCAAGTTTTCTTCATGCAACGGAGAC
  TGCGGTGCAGTTAGGACCAGAAAGCGTGCTATTGTTGGAAAAAGTAAAAAGAAAGAAAAATGTAAAAACT
  CCCATCTGTACCCTCTGATCGAGACCCAGTATTGTCCTTGTGACAAATACAATGCACAGCCAGTGGGGAA
  CTGGTCAGACTGCATTTTGCCGGAAGGGAAGGCTGAGGTGTTGTTGGGAGTGAAGGTACAAGGCGACAAC
  AAGGAATGTGGACAAGGATACCGTTACCAGGCGATGGCGTGCTACGATCAAAATGGCAGACTTGTTGAAA
  CATCGCGATGCAACAGCCATGGTTACATAGAAGAAGCCTGCATCATCCCCTGCCCCTCCGACTGCAAGCT
  CAGTGAATGGTCTAACTGGTCCCGCTGCAGTAAGTCCTGTGGCAGCGGTGTGAAGGTCCGGTCCAAGTGG
  CTGCGGGAAAAGCCTTATAACGGAGGAAGGCCTTGCCCCAAACTAGACCATGTCAACCAGGCGCAGGTGT
  ATGAGGTCGTCCCGTGCCACAGTGACTGCAACCAGTACATATGGGTCACAGAGCCATGGAGTGTCTGCAA
  GGTGACCTTCGTGGACATGCGAGACAACTGTGGAGAGGGTGTGCAGACCCGAAAAGTAAGGTGCATGCAG
  AATACAGCAGATGGCCCTTCTGAACATGTGGAAGACTACCTGTGTGACCCAGAGGATATGCCCCTGGGCT
  CTAGAGAATGTAAACTACCATGCCCTGAGGACTGTGTGATATCCGAGTGGGGGCCATGGACCCAGTGTTC
  TTTGCCTTGCAATCCAAGTGGTTCGAGGCAAAGGTCAGCAGATCCCATCAGACAGCCCGCTGATGAAGGA
  AGAGCGTGCCCTGATGCTGTGGAGAAAGAGCCCTGTAACCTGAACAAAAACTGCTACCGCTATGACTATA
  ATGTCACAGACTGGAGTACATGTCAGCTAAGTGAGAAGGCGGTCTGTGGGAACGGCATTAAAACAAGGAT
  GTTGGACTGTGTTCGAAGTGATGGCAAGTCCGTTGACCTAAAGTACTGTGAAGAGCTTGGGCTGGAAAAG
  AACTGGCAAATGAACAGTTCCTGCACAGTGGAATGCCCTTTGAACTGTCAGCTTTCTGACTGGTCTCCTT
  GGTCAGAATGTTCGCAAACATGTGGACTCACAGGAAAAATGATACGAAGACGAACCGTAACCCAGCCCTT
  TCAAGGTGATGGGAGGCCCTGCCCTTCTCTGATGGAACAATCCAAGCCTTGCCCAGTGAAGCCATGCTAC
  CGGTGGCAATATGGCCAGTGGTCTCCATGCCAAGTTCAGGAGGCCCAGTGTGGAGAAGGAACCAGGACAC
  GGAATATTTCTTGTGTCGTGAGTGATGGCTCAGCTGATGACTTCAGCAAAGCGGTGGATGAAGAGTTCTG
  TGCTAACATCGAGCTCATCATAGATGGTAATAAACAAATAGTTCTGGAGGAAACCTGCACCCAGCCCTGC
  CCAGGTGACTGTTATTTGAATGACTGGTCTTCATGGAGTCTGTGTCAGCTGACCTGTGTGAATGGTGAGG
  ATCTTGGCTTTGGTGGAATACAGGTCCGATCCAGAGCAGTGATTATACAAGAACTGGAGAATCAACACCT
  GTGCCCAGAGCAGATATTAGAAACAAAATCATGTGATGATGGACAATGCTATGAATACAAGTGGATGGCA
  AGTGCTTGGAAGGGCTCTTCTCGAACAGTCTGGTGTCAAAGGTCAGATGGTGTAAATGTAACAGGAGGCT
  GCTTGGTGGTGAGCCAGCCTGATGCTGACAGGTCTTGTAACCCACCCTGTAGTCAACCCCACTCATACTG
  TAGTGAGATGAAGGCATGCCGCTGTGAAGAAGGGTACACTGAAGTCATGTCCTCTAATAGCACCCTTGAG
  CAATGCACACTTATCCCTGTGGTGGTGATTCCCACTGTGGAAGATAAAAGAGATGTGAAAACCAGTCGGG
  CAGTTCACCCAACGCAACCCTCCAGTAACCCAGCAGGACGGGGCAGGACATGGTTTCTACAGCCATTTGG
  GCCAGATGGGAGACTAAAGACCTGNGTTTATGGTGTCGCAGCTGGGGCATTTGTGCTGTTAGTCTTCATT
  GTCTCCATGATTTATCTAGCGTGCAAAAAGCCAAAGAAACCCCAAAGACGGCAGAACAACCGCCTCAAAC
  CTTTAACCTTAGCATATGATGGAGACGCGGACATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001191970
• Insert Size: 4938 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191970.1, NP_001178899.1
• RefSeq Size: 4938 bp
• RefSeq ORF: 4938 bp
• Locus ID: 500032
• Cytogenetics: 4q21