Plekha5 (NM_139340) Rat Untagged Clone

CAT#: RN217575

Plekha5 (untagged) - Rat pleckstrin homology domain containing, family A member 5 (Plekha5)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Plekha5
• Synonyms: Pepp2; TRS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217575 representing NM_139340
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGACCTGAACCTGGAGTGGATTTGTTCGCTGCCGCGCTCCTGGACGTACGGGATCACTAGGG
  GCGGCCGCGTCTTCTTCATCAACGAGGAGGCCAAGAGCACCACCTGGCTGCACCCGGTCACCGGCGAGGC
  CGTGGTCACCGGGCACCGGCGGCAGAGCACAGATTTGCCCACTGGCTGGGAAGAAGCATATACATTTGAA
  GGCGCAAGATACTATATAAACCACAATGAAAGGAAAGTAACCTGCAAGCATCCAGTCACAGGACAGCCAT
  CCCAGGACAACTGTATCTTTGTCGTGAATGACCAGACTGTTGCAACCATGACATCTGAAGAAAAGAAGGA
  GCGACCCATAAGTATGATAAATGAAGCTTCCAATTACAACATGGCTTCAGACTATACTGTGCATCCTATG
  AGCCCCGTGGGCAGGACATCACGGGCCTCCAAAAAGGTTCATAATTTTGGGAAAAGGTCCAATTCAATTA
  AAAGGAACCCTAATGCCCCTGTGGTCAGGCGCGGCTGGCTGTATAAGCAGGACAGCACCGGCATGAAGCT
  GTGGAAGAAGCGCTGGTTTGTGCTTTCTGACCTTTGCCTTTTTTACTACAGAGATGAGAAGGAGGAGGGT
  ATTTTGGGAAGCATCCTGCTGCCCAGTTTTCAGATAGCCATGCTTACTGCCGAGGACCACATCAATCGCA
  AATATGCTTTTAAGGCAGCCCACCCAAACATGCGGACCTATTATTTCTGCACTGATACAGGCAAGGAAAT
  GGAGCTATGGATGAAGGCCATGTTAGATGCTGCTCTGGTGCAGACAGAACCTGTGAAAAGAATTACCTTT
  AATTTCCGAGTGGACAAGATTACAACAGAAAGTGCATCAACTAAAGAAACCAATAACATTCCCAACCACA
  GAGTCCTCATTAGACCTGAGGTCCAAAACAATCAGAAAAATAAGGAAATAAGCAAAATTGAAGAAAAAAG
  GGCCTTAGAAGCTGAAAGATACGGGTTTCAGAAGGATGGGCAAGATAGACCATTAACAAAAATTAACAGT
  GTAAAGTTGAACTCGCTGCCATCAGAATATGAAAGCGGGCCAGCCTGCCCACCTCAGAATATACACTACA
  GACCCATCAATGTGAATAATTCTGACAGCAAAGCAGTGAATGTCAGCTTGGCAGATGTCCGGGGTGGGAG
  TCACCCAAATACAGGGCCCCTAGCCACAGAAGCTGATCGAGTCATTCAGAGAACGAATTCAATGCAGCAG
  CTGGAGCAGTGGATTAAAGTTCAGAAAGGACGGGGTCTTGAAGAGGAGCCCAGAGGAGTAATTTCTTATC
  AAACATTGCCAAGAAATATGCCAAGCCACCGAGCCCAGATTCTGGCCCGCTACCCTGAAGGTTACCGAAC
  ACTCCCAAGAAACAGCAAGACCAGACCAGAGAGTATCTGCAGTGTAACGCCTTCTGGTCATGAGAAGACA
  GGGCCTGGGGCAGAGGAGAAGCGGCGGTCCATGAGGGATGACACCATGTGGCAGCTGTATGAGTGGCAGC
  AGCGCCAGTTTTACCACAAGCAGAGCACCCTCCCTCGGCACAGCTGCCTGAGCAGCCCTAAAGCCATGGG
  GAAGGTTTCTGACCAGACAATGCACTCCATCCCCACGTCACCGTCCCATGTGCCAGCTGCTGCTTACCAG
  GGCTTCTCCCCTCAGCGGACATACAGGTCAGAAGTGTCCTCGCCAATCCAGAGAGGAGATGTGACAATAG
  ACCGCAGGCACAGGCCTCATCACCCTAAGCATGTCTATGTACCTGACAGAAGGTCAATGCCGGCTGGCTT
  AGCCTTACAGGCTGTTAGTCCTCAGAGCCTCCACGGCAGGACGCCAGAGGAGCTTACGCTGCTGCTGATA
  AAGCTGAGACGGCAGCAAGCAGAGCTGAGTAGTATCCGGGAGCACACCTTAGCACAGCTCATGCAGCTGA
  AACTCGAGGCCCACAGCCCGAAGAATGAAATTCTTTCACATCATCTTCAAAGGAACACCATATATTTGGA
  TCATCAGATGAAAGAAAATGAACCTATTATCACCATGGTTCACACCATGATTGAGAACTCGGCGCTAAGA
  CCACAACTGTACCAACAATTCTTAAGACAGAAGAACAAGATAAGTCTCTATTGTCTCTCACAAGATGAGT
  GTAGAGGCACATTATACAAATACAGACCTGAGGAAGTAGATATCGATGCCAAATTAAGCCGATTGTGTGA
  ACAGGATAAGGTGGTCCGTGCACTGGAGGAGAAGCTGCAGCAGCTGCACAAGGAGAAATACACGCTTGAG
  CAAGCCTTGCTGTCAGCCAGCCAAGAGATAGAGATGAATGCAGATAACCCAGCCGCCATTCAGACGGTGG
  TGCTGCAGAGGGACGACCTGCAGAACGGGCTGCTGAGCACGTGTCGGGAACTCTCCCGAGCCACCGCTGA
  GCTGGAACGAGCCTGGAGAGAATATGACAAGTTAGAATATGATGTGACTGTTACCAGGAACCAGATGCAA
  GATCAGCTTGATCGCCTTGGAGAAGTGCAGAGTGAGTCGGCAGGAATTCAACGGGCACAGATTCAAAAAG
  AACTTTGGAGAATTCAAGATGTCATGGAGGGGTTGAGCAAACACAAACAGCAAAGAGGCTCTTCAGAAAC
  AGGTCTGGCAGGACCAAAGCCTTTCCCAGCAGTTAAGTACAAAAGCGAGGAAGAGGAAGTAGTCCCACCT
  CGGCCTCCACTTCCTCGGTCTTATGACTTTACAGAGCAGCCTCCCATAATCCCCCCTCTGCCCAGTGATA
  GCAGCTCCTTGCTCTGTTATAGCAGGGGCCCCGTGCATCTGCCTGAAGAAAAGAAGAGTCACCAAGTTCA
  AGGATATCCAAGAAATGGATCTCACTGTGGTCCAGATTATAGACTCTACAAGAGCGAGCCAGAGTTGACC
  ACAGTGGCAGAAGTTGATGAATCTAATGGAGAAGAAAAATCAGAGCCAGCTTCTGAGGCAGAAGCTCCGG
  TTGTTAGAGGTTCCCACTTTCCAGTTGGAGTTCCTCTGAGAACGAAGTCACCTACACCTGAGTCCTCAAC
  GATAGCTTCCTATGTGACCTTGAGGAAGACGAAGAAGATGGTGGAGCTAAGAACGGAAAGACCGAGAAGT
  GCCGTGGAACAGCTCTGTCTGGCGGAAAGTACTCGGCCTCGGATGACTGTGGAAGAGCAGCTGGAGAGAA
  TCAGGAGGCACCAGCAGGCCTGCCTGAGGGAGAAGAAGAAGGGGCTGAACGTCCTCAGTGCATCCGACCC
  ATCTCCCTCACAAAGTCCTTCAAGCGTGAGGGACAGTCCTTCCAGAGTTCCTCAGACTGTAAGGAGGGAT
  GATAACACTAAGGAGCTAGACGCCGTCCACAGAGAAAATGATGGAAAGCCAGACTATGACACTTCAGCAG
  CAGAAACTGCTCACCCAGAAGAAGCTGAACCCCAAAGTGCAGATGTCGGCCGAAAGCTAAAGAGAACTGA
  AAGTATTTTTTATGAAATGCTTTACACGCCTGAACCTTTAAATGGACTGACTTCTGAGGAAGTGATGGCT
  AAGGAGAGGCAGGAAGAGCAGGTGCCTGAAGGTGCCGTGTGCAGCCTTCAGGAAGAGGCTGCCATGGCAA
  ACCATCAGGCAGAAGGCCCCCCTGAGGAAGCAGAGAGTCTTCATGAAGAGGAGGAAGCTCTGGCTTCCTG
  TGAGCCAGCTCCTGAGACTCCTAGGGAGAATCAAACCACAGTGAAAAGTCTGTCTCCGTCTCCTGACTCC
  TCCACGGCAGCAGACCCGCCCACTCCGCCCCAGCTCACAGAAGGATCACATTTCATGTGTGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_139340
• Insert Size: 3846 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139340.2, NP_647556.1
• RefSeq Size: 4790 bp
• RefSeq ORF: 3846 bp
• Locus ID: 246237
• Cytogenetics: 4q44
• Gene Summary: member of homeobox PEPP subfamily, preferentially expressed in reproductive tissues; human homolog specifically expressed in testis [RGD, Feb 2006]