Nfasc (NM_001160313) Rat Untagged Clone
CAT#: RN217536
Nfasc (untagged) - Rat neurofascin (Nfasc), transcript variant 2
"NM_001160313" in other vectors (1)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Symbol | Nfasc |
Synonyms | NF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN217536 representing NM_001160313
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCAACAACCCCTATA ATGACTCGTCTTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCGGAAAGAACACCTAGCTT CATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATT GCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCA AGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGG CTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGA ACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGGCACCACCCAACCCCAACCG TGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAAC ACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGC TGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGG GTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCAC GTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG CCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG GATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCC TCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATTGGAAACAGGATGAAAAAGG AAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCAC CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGCTGATCAGGCCACTCCGACTAACCGT TTGGCTGCCCTGCCCAAAGGGCGGCCAGACCGACCCAGGGACCTGGAGCTCACTGACCTGGCGGAAAGGA GTGTGAGGCTGACCTGGATCCCGGGGGATGATAACAACAGTCCCATCACAGACTACGTTGTCCAGTTTGA AGAGGACCAGTTCCAGCCAGGAGTCTGGCATGACCACTCCAAGTTCCCAGGCAGTGTCAACTCAGCCGTC CTCCATCTGTCCCCGTATGTCAACTATCAGTTCCGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACC CCAGCCTTCCATCCGAGCGGTACCGAACAAGCGGGGCACCCCCTGAATCCAACCCCAGTGATGTGAAGGG CGAAGGGACACGAAAAAACAATATGGAGATCACATGGACGCCTATGAATGCTACCTCCGCCTTTGGCCCC AACCTGCGCTACATTGTCAAGTGGCGACGGAGAGAAACCCGAGAGACTTGGAACAATGTCACCGTGTGGG GCTCTCGCTATGTGGTGGGGCAGACCCCTGTCTACGTACCCTATGAGATCCGAGTCCAGGCTGAAAATGA CTTTGGGAAAGGCCCGGAGCCTGAAACCGTCATTGGGTACTCGGGGGAAGATTATCCCAGGGCTGCACCC ACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTTCAGTGGAACCGCGTCTACCCTGACA CGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGGAAAGCAGTTTGCTGAAGAACCTGTG GGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGCGACCGCCCCCGGGGCGTGGTGGGTCGCCTGTTC CCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGAGGTGATGGGCCTCGAAGTGAAACCA AGGAATTCACCACCCCGGAAGGAGTACCCAGTGCCCCCAGGCGTTTCAGAGTCCGACAGCCCAACCTGGA GACCATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGATCCTGATTGGATACACGCTCAGATAC GTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCT CGGTGCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCAGTGCCAGGACGCAGGTGGGCTCTGG AGAAGCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTACTCCAACTGCAGCTTACACCAACAAC CAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTTCTGGTGCTGATCC TGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACCCAGTGCGAGAAAAGAAGGATGTCCC CTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTACAGTGACGAGGACAACAAGCCCCTG CAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAGAGTGACGACAGCCTAGTGGACTACG GCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTGGCCAGTACACTGTCAGAAAGGACAA GGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGTCAATGCCATCTATTCCCTGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001160313 |
Insert Size | 3570 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001160313.1, NP_001153785.1 |
RefSeq Size | 9454 bp |
RefSeq ORF | 3570 bp |
Locus ID | 116690 |
UniProt ID | P97685 |
Cytogenetics | 13q13 |
Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009] Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The promoter and 5' UTR for this variant have not been determined. The resulting isoform (2) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences near the N-terminus, compared to isoform 1. Isoform 2 is also known as NF155, as described by Davis et al. (PMID: 8947556). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR217536 | Nfasc (myc-DDK-tagged) - Rat neurofascin (Nfasc), transcript variant 2 |
USD 1,138.00 |
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