Nfasc (NM_001160313) Rat Untagged Clone

CAT#: RN217536

Nfasc (untagged) - Rat neurofascin (Nfasc), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nfasc
• Synonyms: NF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217536 representing NM_001160313
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT
  GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT
  CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG
  TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA
  ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC
  CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT
  CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA
  CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC
  TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCAACAACCCCTATA
  ATGACTCGTCTTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCGGAAAGAACACCTAGCTT
  CATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGACCTGCTGCTGGAGTGCATT
  GCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGACAAGGCCA
  AGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG
  CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCCCCATACTGG
  CTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTGGTGTGTCGAGCCAATGGGA
  ACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGGCACCACCCAACCCCAACCG
  TGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAGGGCAGTGTACCAGTGTAAC
  ACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTAGATGTACCCCCTCGGATGC
  TGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGACTGGACTGTCCGTTCTTTGG
  GTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGTAACTACCAC
  GTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG
  CCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG
  GATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTGCCGTGTGAAGCATGACCCC
  TCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATTGGAAACAGGATGAAAAAGG
  AAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTTACACGTGCATGGCCAGCAC
  CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGCTGATCAGGCCACTCCGACTAACCGT
  TTGGCTGCCCTGCCCAAAGGGCGGCCAGACCGACCCAGGGACCTGGAGCTCACTGACCTGGCGGAAAGGA
  GTGTGAGGCTGACCTGGATCCCGGGGGATGATAACAACAGTCCCATCACAGACTACGTTGTCCAGTTTGA
  AGAGGACCAGTTCCAGCCAGGAGTCTGGCATGACCACTCCAAGTTCCCAGGCAGTGTCAACTCAGCCGTC
  CTCCATCTGTCCCCGTATGTCAACTATCAGTTCCGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACC
  CCAGCCTTCCATCCGAGCGGTACCGAACAAGCGGGGCACCCCCTGAATCCAACCCCAGTGATGTGAAGGG
  CGAAGGGACACGAAAAAACAATATGGAGATCACATGGACGCCTATGAATGCTACCTCCGCCTTTGGCCCC
  AACCTGCGCTACATTGTCAAGTGGCGACGGAGAGAAACCCGAGAGACTTGGAACAATGTCACCGTGTGGG
  GCTCTCGCTATGTGGTGGGGCAGACCCCTGTCTACGTACCCTATGAGATCCGAGTCCAGGCTGAAAATGA
  CTTTGGGAAAGGCCCGGAGCCTGAAACCGTCATTGGGTACTCGGGGGAAGATTATCCCAGGGCTGCACCC
  ACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTTCAGTGGAACCGCGTCTACCCTGACA
  CGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGGAAAGCAGTTTGCTGAAGAACCTGTG
  GGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGCGACCGCCCCCGGGGCGTGGTGGGTCGCCTGTTC
  CCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGAGGTGATGGGCCTCGAAGTGAAACCA
  AGGAATTCACCACCCCGGAAGGAGTACCCAGTGCCCCCAGGCGTTTCAGAGTCCGACAGCCCAACCTGGA
  GACCATCAACCTGGAATGGGATCACCCAGAGCACCCCAACGGGATCCTGATTGGATACACGCTCAGATAC
  GTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCT
  CGGTGCAGAGAGCAGACCCCGTGTCCCGTTACCGCTTCTCCCTCAGTGCCAGGACGCAGGTGGGCTCTGG
  AGAAGCAGCCACGGAGGAGTCCCCAACACCTCCAAATGAAGCTACTCCAACTGCAGCTTACACCAACAAC
  CAGACTGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTTCTGGTGCTGATCC
  TGCTGATCGTCTGCTTCATCAAGAGGAGTCGTGGCGGCAAGTACCCAGTGCGAGAAAAGAAGGATGTCCC
  CTTGGGCCCTGAAGACCCCAAAGAAGAAGATGGTTCATTTGACTACAGTGACGAGGACAACAAGCCCCTG
  CAGGGCAGCCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAGAGTGACGACAGCCTAGTGGACTACG
  GCGAGGGTGGCGAGGGCCAGTTCAACGAAGATGGCTCCTTTATTGGCCAGTACACTGTCAGAAAGGACAA
  GGAGGAGACCGAGGGCAATGAGAGCTCAGAGGCCACATCTCCAGTCAATGCCATCTATTCCCTGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001160313
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160313.1, NP_001153785.1
• RefSeq Size: 9454 bp
• RefSeq ORF: 3570 bp
• Locus ID: 116690
• UniProt ID: P97685
• Cytogenetics: 13q13
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]
  Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The promoter and 5' UTR for this variant have not been determined. The resulting isoform (2) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences near the N-terminus, compared to isoform 1. Isoform 2 is also known as NF155, as described by Davis et al. (PMID: 8947556). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.