Lamb3 (NM_001100841) Rat Untagged Clone

CAT#: RN217523

Lamb3 (untagged) - Rat laminin, beta 3 (Lamb3)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Lamb3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217523 representing NM_001100841
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACGGCACTTTTCCTCTTGTGGCTTGCCTTGCCTGGCCTCCTGTGTGCCCAGCAACCCTGCTCCC
  GAGGGGCCTGTTATCCACCTGTTGGGGACTTGCTCATTGGAAGGACTCAGCTTCTTCGAGCCTCGTCTAC
  CTGTGGATTGACCAAGCCTGAGACCTATTGCACCCAATATGGACAGTGGCAGATGAAATGCTGCAAGTGT
  GACTCCAGGCTGCCTCGCAACTACAACAGTCACCGAGTGGAGAACGTTGTATCGTCTTCAGGCCCCATGC
  GCTGGTGGCAGTCCCAGAACGATGTGAGCCCTGTCTCTCTTCAACTGGACCTAGACAAGAGGATGCAGCT
  TCAGGACATAATGATGGATTTTAAGGGTCTCATGCCAGCTGGCATGTTGATTGAGCGCTCTTCTGACTTT
  GGCAAGACATGGAGGGTGTACCAGTACCTGGCGACAGACTGTGCCAGCACCTTTCCCCAGGTCCACCAGG
  GCCAGCCCAAGAACTGGCAGGACGTCCGGTGCCGGCCCTTGTCCCAGAGGCCTAATGGGCATCTGACTGG
  GGGAAAGGTCCAACTTAACCTTATGGATTTGGCATCAGCTATCCCTGCATCTCAAAGTAAAAAGATTCAA
  GAACTAGGAGACATCACGAACTTGAGAGTCAACTTCACCAAGCTGGCCCCAGTGCCCCAGAGGGGCTCCT
  ATCCACCCAGCGCCTACTTTGCAGTATCACAGCTACGTCTACAGGGGAGTTGCTTCTGTCATGGACATGC
  CGACCGCTGTGCCCCTAATCCTGGAGGCCCTACAGGTTCCACCACCGCTGTGCAGGTCAACGATGTCTGT
  GTCTGCCAGCACAACACAGCTGGCCCCAACTGTGACCGCTGCGCCCCCTTCTACAACAACCGGCCATGGA
  GACCTGCAGAGGGACAGGACATCCATGAGTGCCAAAGGTGTGACTGCAATGGGCACTCAGAGACCTGCCA
  CTTCGACCCAGCTGTGTTTGCTGCTAGCCAAGGGACAAGTGGAGGCGTGTGTGACAACTGCCAGGACCAC
  ACAGAGGGCAAGAACTGTGAGCGATGTCAGCTACACTATTTCCGGAATAGACGACCAGGCGCTCCCATTC
  ATGAGACCTGTATTCCCTGCGAGTGTGATCCAGACGGGGCAGTCCCAGGAGCTCCCTGTGACCGACTGAC
  GGGCCAGTGCGTGTGCAAAGACCACGTGCAAGGGGAGCGCTGTGATCTTTGCAAGCCTGGTTTCACAGGG
  CTCACCTTCGCCAACCCACAGGGCTGCCATCCCTGTGACTGCAGCATCTTAGGTACCCGGCAAGATATGC
  CTTGTGAGGAGGAAACTGGACGCTGCTTGTGTCTTCCCAATGTAGTGGGCCCCAAATGTGACCAGTGCGC
  CCCTTCCCACTGGAAGCTGGCCAGCGGCCGGGGCTGTGAGCCCTGTGCCTGTGACCCACGCAACTCCCTC
  AGCTCCCAGTGCAACCAGTTTACAGGGCAGTGTCCTTGTCGGGAAGGGTTTGGTGGCCTCACATGTAGTT
  CTGCAGCCATTCGTCAGTGTCCCGACCGGACCCATGGAGACGTGGCCACAGGGTGCCGAGCCTGTGACTG
  TGACTTCAGAGGAACCGAGGGTCCTGGCTGTGACAAAACCTCAGGCCGTTGCCTCTGCCGCCCCGGCTTG
  ACAGGGCCCCGCTGCGACCAGTGCCAACGAGGCTACTGCGACCGCTACCCAGTGTGTGTGGCCTGCCACT
  CTTGCTTCCAGGCCTATGACACAGACCTCCAGGAGCAGGCTTGGCGCCTTGGCAGTCTCCGTAATACCAC
  TGAAGGCCTGGGCACAGGGACAGGTCTGGAGGACCGTGGCCTGGCTTCTCGGTTGCTAGATGCCAAGAGC
  AAAATCGAGCAGATCAGACAGATTCTGGGTGGCACCTCTGTCACAGAGCGGGATGTGGCTCAAGTGGCCA
  ATGCCATCCTGTCTATCAGGAGGACTCTTCAGGGCCTGCCCCTGGACCTACCCTTAGAGGAGGAGATGGA
  ATCCTTCTCTGGAGACCTGGGGAATCTAGACAGAAGCTTCAATCGCCTCCTCCTTATGTACCGGAGCAAG
  AAGGAGCAGTTTGAGAAGCTAAGCAGTGTGGACCCTTCAGGAGCCTTCCGCATGCTGACTATGGCTTATG
  AGCAGTCCTCCCGGGCTGCACAGCAAGTATCCGACAGTTCCAGCCTGCTGAGCCAGCTGAGGGACAGTCG
  CAGAGAGGTAGAAGGTCTGGAGAGACAGACTGGAGAGGGAGGAGCTGGAGGAGCTCAGCTCATGGCCCTG
  CGCCTAGAGATGGCTTCACTGCCTGACTTGACACCCACCATCAACAAGCTCTGTGGTGGCTCTAGGCAGA
  CAGCCTGCACCCCAGGAGACTGCCCTGGATTGCTGTGTCCCCAGGACAATGGTACAGCCTGTGGCTCTCA
  CTGCAGGGGAGCCCTGCCCAGAGCCAGAGGGGCCTTACACATGGCAGGGCAGGTAGCTGAGCAGCTACGA
  AGCTTCAATACCCAGCTCCAGCAGACCAGGCAAATGATCAGGGCAGCTGAGGAGGCAGCATCAAAGGTCC
  AATCAGATGCCCAGCGCCTTGAGACTCAGGTGAGTACCAGCCGCTTACAGATGGAGGAAGATGTCCGGCG
  CACACGGCTTCTCATCCAGCAGGTCCGGGGCTTCCTCACAGACCCTCACACAGATGCAGCCACCATCCAA
  CAGGTGAGCGAGGCAGTGCTGGCTCTCTGGCTGCCCACAGACTCAGCTACAGTGCTACGCAAGATGAAAG
  AGATCCAGGACATTGCTGCCAGGCTCCCTAACGTGGACTTAGTGCTATCCCAGACTAAGCAGGACATTGC
  ACGGGCCCGCAGGCTCCAGGCTGAGGCCGAGAAGGCCAGAAGCCGAGCCCACGTTGTGGAAGGGCAGGTG
  GATGACGTGGTCGGGAACCTCAGGCAGGGCACCGTGGCTCTGCAGGAGGCTCAGGACACTATGCAGGGCA
  CTGGCCGCTCTCTTCGGCTCATCCAGGAAAGGGTTACTGAGGTTCAGCAGGTCCTGGTACCAGCTGAAAG
  GCTGGTGAAAGGCATGAAGGAACAGATGAGTGGATTCTGGGCACGGGTGAAGGAGCTCCGCCATCAGGCC
  CAGGAAGAGCAGGCACAGGCAATGAAGGCCCAGCAGCTTGCAGAGGGTGCCAGCAATCAGGCCATGAATG
  CTCAGGAGGGCTTTGAGAGACTTAAGCAAAGGTATACAGAGTTGAAAGACCGGCTGGGTCAGAGTTCTAT
  GCTGGGTGAGCAAGGCAATCGGATCTTGAGCATCAAGGCGGAGGCAGAGGAGCTCTTTGGGGAAACCATG
  GAAATGATGGATAAAATGAAAGACATGGAGTCAGAGCTGCTTCGAGGGAGTCAGGCCATCATGCTTCGTT
  CAGCAGACCTGTCGGGGCTGGAGAAGCGCGTGGAGCAGATCCGCAGTTACATCAACGGGCGTGTGCTGTA
  CTATGCCACCTGCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001100841
• Insert Size: 3519 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100841.1, NP_001094311.1
• RefSeq Size: 4078 bp
• RefSeq ORF: 3519 bp
• Locus ID: 305078
• Cytogenetics: 13q27