Tmc3 (NM_001170432) Rat Untagged Clone

CAT#: RN217475

Tmc3 (untagged) - Rat transmembrane channel-like 3 (Tmc3)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tmc3
• Synonyms: Stard5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217475 representing NM_001170432
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACCTCAAAGGCATCCCAGCGCTACAGAAGCATCCGGAGAAATGCCAGCCAGTGCTACCTCTACC
  AGGACTCCCTGCTGCTTGGTAACTTCGACGACAGCTTTAATGCTGACGAAGCAGGAGACAGCAGTGATCC
  AGAACAAATCTTCCAGAATATACAGTTTCAGAAAGATCTCATGGCAAATATCCGCTGCAGACCCTGGACC
  ATGGGGCAGAAGCTTCGGGCACTGAGACGAGCAAAGGAGATCGTATTGAAGTTTGAAGGCAGGCTGACCA
  GGACTCGAGGCTACCAAGCAGCGGGAGCAGAGCTCTGGCGGAAATTTGCCCGTCTGGCCTGTAACTTTGT
  GGTCATCTTCATCCCCTGGGAAATGAGGATAAAGAAAATCGAGAGTCACTTCGGATCTGGTGTGGCCTCC
  TACTTCATCTTCCTGAGGTGGTTATTCGGTATTAACATAGTGCTCACGGTGATGACGGGTGCCTTTGTTG
  TCCTTCCTGAGCTAATCGCAGGCCAGCCCTTTGGAAGCACAGCCAGCAAGACCATCCCCCAGGAGCAGGT
  CATGTCTGCACAGGATCTGGACACTGTCTGGTCCCTCGGGGGCTACCTTCAATACTCTGTCCTGTTCTAT
  GGATACTACGGAAGAGAGAGGCGGATCGGGAGAGCCGGCTACCGCCTGCCCTTGGCATATTTCCTGGTGG
  GGATGGCAGTGTTTGCCTACAGCTTCATCATTCTCTTAAAGAAAATGGCTAAGAACTCCCGCACCAGCCT
  GGCCAGTGCTTCCAATGAAAACTACACCTTCTGCTGGCGGGTGTTTTGTGCCTGGGATTACCTCATTGGG
  AACCCAGAGGCTGCAGAAAGCAAAACAGCTGCCATCCTGAACAGCATTAGGGAGGCCATACTGGAAGAGC
  AAGAAAAGAAAAAAAACAAAAACATGGCGGTGACCGTCTGCCTGAGAATCATTGCCAACATCCTGGTGCT
  CCTCTCGCTGGCTGGGAGCATCTATCTCATCTACTTTGTGGTGGACCGGTCCCAGAAGCTGGAGCAGTCG
  AAGAAGGAGCTGACACTTTGGGAAAAGAATGAGGTAGGCGTGGTGGTCTCCCTTGTCACCATGCTAGCAC
  CATCTGCCTTTGACCTCATCGCTGCCTTGGAGATGTACCATCCACGGACGACACTGCGCTTCCAGTTAGC
  AAGGGTCCTGGTACTGTATCTAGGAAATCTCTACAGCTTGATCATTGCTCTTCTGGACAAAGTGAACAGC
  ATGAACATTGAGGAAGCTGCTACTAAAAACAGCACAAGCCACTGGGCAGATGCTCCCACCTTCTATGCCA
  CCAGGACAGTGCCTGAAGAAGGGCAGTGGCCCACACCTGTGTCAGGGGTGGAGCTCAGGAGAAACACCAG
  CATGTGGGACTTGGAAGAGACAAGCTTTCTGACTTCTATCACACCACACACAGTAGCCAACAAGACAGTG
  TCCTACATGCAGGGCCCACAAGGCCAGTGCTGGGAGACATACGTCGGCCAGGAGATGCTGAAGCTGTCTG
  TCATTGACATGCTCTTCACTGTGGCCAGCATCCTCCTCATAGACTTCTTCCGAGGACTTTTTGTTCGCTA
  CTTAAGTGACTATTGGTGTTGGGACCTTGAAAGCAAGTTTCCTGAATATGGAGAATTCAAGATTGCTGAG
  AATGTGCTGCATCTTGTCTACAATCAAGGCATGATTTGGATGGGGGCCTTCTTCTCTCCGTGTCTGCCAG
  CATTCAATGTTCTCAAGCTCATTGGGCTGATGTACCTGAGGAGCTGGGCAGTGCTGACCTGCAATGTACC
  CCACCAGCAAGTGTTTCGAGCTTCCAGATCCAACAACTTCTACCTGGCAATGCTCCTGTTCATGCTGTTC
  CTCTGCATGCTGCCAACTGTCTTTGCGATTGTCCACTACAAGCCGTCCCTGAACTGCGGCCCCTTCAGCG
  GACAAGAGAAAATTTATGACATCGTGTCAGAGACGATAGAGAATGACTTCCCCACGTGGTTTCACGTCGT
  TGTTGGACACATCAGCAGCCCCGTGGTGATACTCCCAGCTGTGCTGCTTCTGTTCATGCTCATCTACTAC
  CTTCAGAGCATCGCGAGATCACTCAAGCTCAGCAGCCAGCAACTCAGGATGCAGATCCAAAACGCGAGAT
  CAGAAGATAAGAAAAAAGTTGCCCAAATGGTAGAGGCTCGAATCCAGACTCATGAAGAAAGCAGCAAGAG
  GCTTCTAAAGGACAGTGACCTTATCAGCCAGCTGTCCTCTGCATACCTGGCAACATCCCATAACAATGGC
  AACATGCTCAACTTTGACTCTCTGAGTAGCAAGAGCCTCAGGATGGAGACAATTACAAGGTCCCTGCCCC
  AGAGTCCTGGACAAGGGTCCAGAGGCCCCTGCTCTCCTCTTCCAGATGGATCCAGATCAAGACCAGAGCA
  GGACACCAATAGGAATCCACACGGGCCGTGTTCCAGCACAGGCAACCTCCACAAGAACAGATCCTGCTCA
  TCTGTCACACAAACACAGCCCCTCAAAGATGCTCGCTCAGAGCCCCTTTCCAGAAAAAACTTTCAGCCAA
  TCCGTCCTCCTTTCTGTGGATCTGAAGTCTCTGCCTTGATGACACATGGCCACGGTCTCCGTGCTCCCAG
  ATACTATGTCGTCAATGAGCATGACTCTCACAAAAAGACCCACTCAGCTTTCTGGCCAGAAAGACATTTC
  AAGATAGATGCTTTAGGTGACATTGTTGAGTTGTACCCCAGGAATGTAGGGCAGTACATGTCCTGGGTTC
  CCCACCAGCCTTCTTCTCCACAGCTGAGTGAAGAAGAGGAGGAGATACTGAGAAGAGATTTGATCCAGTG
  GTCAATCCCTGCTAGCTCCCTGACAGACCTCCCTCGGTCTTCTTGTTTTTACACTGGTGACAGATCAGAA
  AATAACACCAGAGACCCTAAGTACCAGCGAAGGGTGTACTACAGATCTGGGGAAAATAGTTTTGAAGGCC
  AGCTTGAGAGGCCAATATTTGTACACAAAAAGCCACGACCCAGAAATGTCCAATACCCACAGCATCCCCT
  GAAGGCCAAAGTCAAAGCTAAGTTCGAACCATCGTTCACAGAATCTGATTCTGTGTCAGCAGCCTCCAGC
  AGTGACCAGCAAAACAGCAACAATGACCAATACAGGCATGTCATGTCCAGCCAGGCAAGATTCCCCAGGT
  CTGCCAGCCAGCTGGGCAGGAGGAAGGCCAAGTACAGGCAGGTACTGCCCACAGATCTAAATGACCTAAT
  TTGTTCAAATGTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170432
• Insert Size: 3306 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170432.1, NP_001163903.1
• RefSeq Size: 3306 bp
• RefSeq ORF: 3306 bp
• Locus ID: 293065
• Cytogenetics: 1q31