Dennd1a (NM_001191747) Rat Untagged Clone

CAT#: RN217419

Dennd1a (untagged) - Rat DENN/MADD domain containing 1A (Dennd1a)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dennd1a
• Synonyms: RGD1307927
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217419 representing NM_001191747
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTGTTTCCTTTGTAGACAAAACCCGGAGACTACATTTGAAGTGTATGTGGAAGTGGCCTATCCTA
  GGACAGGTGGCACGCTTTCAGATCCTGAGGTGCAGAGGCAATTCCCGGAGGACTACAGTGACCAGGAAGT
  TCTACAGACTTTGACCAAGTTTTGTTTTCCCTTCTATGTGGACAGCCTCTCAGTTAGCCAAGTTGGCCAG
  AACTTCACATTTGTGCTCACTGACATTGACAGCAAACAGAGATTCGGGTTCTGCCGCTTATCTTCAGGAG
  CCAAGAGCTGCTTCTGTATCTTAAGCTACCTCCCCTGGTTTGAGGTATTTTATAAGCTCCTTAACATCTT
  GGCAGATTACACAACAAAACAACAGGAAAGTCAGTGGAATGAACTTCTTGAAACTCTGCACAGACTTCCC
  ATTCCAGACCCCGGAGTGTCTGTTCACCTCAGCGTGCATTCTTACTTCACTGTGCCTGATAGCAGAGAGC
  TTCCCAGCATACCTGAGAATAGAAATCTGACAGAATATTTTGTGGCTGTGGATGTGAACAACATGTTGCA
  TCTGTACGCCAGCATGCTGTATGAGCGCCGGATCCTCATCATCTGCAGCAAGCTGAGTACCTTGACGGCC
  TGCATCCACGGCTCTGCTGCCATGCTCTACCCCATGTACTGGCAGCATGTGTACATCCCTGTGCTGCCCC
  CACATCTGCTGGACTACTGCTGTGCTCCCATGCCCTACCTCATAGGAATCCATTTAAGTTTAATGGAGAA
  GGTCAGAAACATGGCCCTAGATGATGTTGTGATCTTGAATGTGGACACCAACACCCTGGAAACCCCTTTT
  GATGACCTTCAGAGCCTCCCAAATGATGTGATCTCCTCCCTGAAGAACAGGCTGAAGAAGGTCTCCACAA
  CCACAGGCGATGGGGTGGCCAGAGCCTTCCTCAAGGCCCAGGCTGCTTTCTTTGGCAGCTACCGGAATGC
  ACTGAAAATTGAGCCAGAAGAACCCATCACTTTCAGTGAGGAGGCCTTCGTGTCCCACTACCGCTCTGGA
  GCCATGAGACAGTTCCTACAGAATGCCACACAGTTGCAGCTCTTCAAGCAGTTTATCGATGGCCGACTAG
  ACCTTCTCAATTCCGGTGAAGGTTTCAGTGATGTCTTTGAGGAGGAGATCAGCATGGGAGAATATGCCGG
  CAGTGATAAACTGTACCATCAGTGGCTGTCCACAGTCCGGAAAGGAAGTGGAGCGATTCTGAATACTGTA
  AAAACAAAAGCTAACCCAGCCATGAAGACTGTCTACAAGTTTGCAAAAGATCATGCAAAAATGGGAATAA
  AAGAGGTGAAAAACCGCTTGAAGCAAAAGGACATCACCGAGAATGGCTGTGCATCCTCTGCAGAAGATCC
  ACTGCCAAAGACCATACCATCCCCACTGGCAGAGGCCAAGGACCCCAAACTTCGAGAAGACCGGAGACCA
  ATCACAGTCCACTTTGGGCAGGTAAGACCGCCTCGGCCGCATGTCATCAAGAGACCCAAGAGCAACATGG
  CTGTGGAAGGCCGGAGGACGTCCATCTCAAGCCCCGAGCAGCCACAGCCATACAGGACTTTGAAAGAATC
  AGACAGCGCAGAAGGTGATGAGACCGAGAGTCCAGAGCAGCCAACTCGGGAGCCCTGGGGGCCTACCCCT
  GCTCCACCTGACCGAGCTGCCAGCATTGACCTTCTAGAAGATGTCTTCAGCAGCCTAGATGTGGAGGCAC
  CACTGCAGCCGCTGGGCCAAGCCAAGAGCCTGGAGGATCTTCGGGTCCCCAGAGACTTGCGGGAACAGTC
  AGGCAGCTTTGATTACCAGAGGCTGGACCTATGCAGAAGTGAGAGGGGCCTTAGCATGGCTGCAGCCTTG
  AAGTTTGCTCATCCATATACTCAGCTCTGGAGTCTCGGACAAGACGACATGGCCATCCCCAGCAAGCCCT
  CCATGACCTCCCCTGAGAAGCCCTCAGCTCTGCTTGGTACTTTGCCAGCACTGCCTGTCAGACCCCAGAA
  CCAGGAGGGCATCCTGAGCCCCAGCATCAAGGAGGAGACTCCCGTGCCAGCTCCAGGCAGCATCACCATT
  CCCAGGCCACAAGGCAGAAAGACCCCTGAGCTTGGCATTGTGCCTCCACCACCCACTGCCCGGCCAGCCA
  AGCTCCAGGGTGCTGGCGGCCCACTTGGTGACTTCTCTTCAGAGCCACTACAGATGGACCTGGAAAGACA
  AGCTGCTCTGAGTCCAGCCCTCTTGTCAGGGCTGCTCCCGAGAGCTGGTCCCCAAGGCTCCACAGAACTT
  GTGCAGTCACCCAGCCCAGCCCCAGGAGCTGCAGGCACAGGCAGCGATGCCCTGCTTGCTCTCCTGGACC
  CACTTAACACAGCCTGGTCAGGCAGCACCATCCCATCCTATCCTACTACCCCCAGTGTAGCCACCCCATT
  CACCCCCCAGCTCAGCTTCCCCTCAACAGTGACACCCTCCCCTTTTGCACAGACACCACTCAATCCCTTT
  GTCCCCTCTGTGCCAGTAGTACCACCCACCATGTCCCTGGGCTCTACACCAGCCAGACCTTTTGGGACGC
  CTCCAGCATCCCTAGGGCCAGCATATGCACCTAGCATCCTATTGTCTGGTTCTAGCTTCTGTGCCCCTCA
  CAGGTCTCAGCCCACCCTCTCTGCTCTCTCTATGCCCAACCTCTTTGGCCAGATCCCAATGGGTGCCCAC
  ACTAGTCCCCTGCAGCCACTTGGCCCCCCAGCCGTTGCCCCCTCCAGGATCCGAACATTGCCTCTGCCCC
  GCTCAAGTGCCAGGGCTGCTGAGGCCAAGCAGGGGTTGGCCCTGAGGCCAGGTGAATCCCCACTGCTGCC
  TCCTAGACCACCACAGAATCTACAGCCAACCCTGCAGCCCTCTGCTCCTACACAGGCCAGAGACCCCTTT
  GAGGATTTGCTACGGAAAACCAAGCAAGATGTGAGCCCAAGCCCAGTCCCGGCCCCGGCCCCAGCCCCAG
  CCCCAGCCTCAGCCTCCACCTCTGTGGAGCAGCTCCGCAGGCAGTGGGAGACCTTCGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001191747
• Insert Size: 3072 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191747.1, NP_001178676.1
• RefSeq Size: 4114 bp
• RefSeq ORF: 3072 bp
• Locus ID: 311913
• Cytogenetics: 3q11-q12