Ndst3 (NM_001191716) Rat Untagged Clone

CAT#: RN217285

Ndst3 (untagged) - Rat N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (Ndst3)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ndst3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217285 representing NM_001191716
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGAGTTTTATCATGAAACCTCACAGACACTTTCAAAGAACACTGATTCTGCTTGCCACCTTTTGTATGG
  TGAGCATCGTTATTTCTGCTTACTACCTGTACAGCGGCTACAAACAGGAAAGTGAAGTCTCTGGGCGGGC
  TTCGGTAGTGGACTGTGGTGACCTCCAGCACATACCATCCAGGCTGATGGAAGCGAAGAGGACAATGATT
  TCTGATGCTTCAAGGACAGACCCCACAGTCCTAGTGTTTGTGGAAAGCCAGTACTCATCTCTTGGTCAAG
  ACATCATTATGATGCTAGAATCCATCCGGTTCCATTATCACACAGAAATCGCTCCTGGAAAAGGCGATCT
  TCCAGCGCTTACAGACAATGTGAAAGGCAAATACATTCTCATTATATATGAGAATATTCTAAAGTATATA
  AACATGGACTCTTGGAATAGAAGCCTCTTAGATAAATACTGTATAGAGTATGGTGTGGGTATCATTGGAT
  TCCACAAAACCAATGAGAAAACCCTTCAGAGCTTTCAATCCCAGGGCTTCCCCTTTTCCATAAGTGGAAA
  CCTGGCAGTAAAAGATTGCTGTATTAATCCTCACTCCCCTCTCCTTCGTGTGACCAAATCATCCAAGCTT
  GACAGAGGTTCTTTACCTGGAGCTGACTGGACAGTTTTTCAGATTAATCACTCCACCTACCAGCCAGTAA
  TATTTGCCAAAGTTAAGACTCCAGAAAACCTTTCTCCTCCCATCTCTAAACATGCATTTTATGCCACTAT
  CATACATGACCTGGGGCTTCATGACGGGATCCAGCGAGTTCTTTTTGGCAACAACTTGAACTTTTGGCTG
  CACAAGCTCATTTTCATAGATGCCCTCTCCTTCTTGTCAGGGAAAAGACTGACACTGTCCTTGGACAGGT
  ACATCCTTGTGGACATTGACGACATATTTGTGGGGAAGGAGGGCACAAGGATGAACACCAATGATGTCAA
  GGCCCTGCTCGACACTCAGAATCTTTTGCGCTCACAAATCACCAACTTTACTTTCAACCTTGGATTTTCA
  GGGAAATTTTATCACACGGGAACCGAAGAAGAAGATGCAGGGGATGACTGTCTGTTGGGGTCTGTGGATG
  AGTTCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCATCTCTTCCATAATGAGTCGTCTTTAAT
  AGAGCAGATGATTCTCAACAAAAAGTTCGCCTTAGAGCATGGCATCCCTACGGACATGGGCTACGCAGTG
  TCCCCTCACCATTCAGGTGTCTACCCCGTACACGTTCAACTTTATGAGGCCTGGAAGAAGGTTTGGAATA
  TTAAAATCACCAGCACTGAAGAATATCCACATCTGAAACCAGCTAGGTATCGGAGAGGCTTCATCCACAA
  AAACATCATGGTTCTCCCGAGACAAACCTGTGGACTCTTCACCCACACGATTTTCTACAAGGAGTACCCA
  GGGGGCCCCAGGGAACTGGACAAGAGTATTCATGGAGGGGAGCTCTTCTTCACTGTGGTTCTCAACCCAA
  TCAGTATTTTCATGACCCACTTGTCTAACTATGGAAACGACCGGCTGGGATCATACACATTTGTGAACCT
  AGCCAATTTCGTGCAGACCTGGACCAACCTGCGCCTTCAGACCCTGCCTCCGGCTCAGCTGGCTCACAAA
  TATTTTGAGCTCTTTCCTGATCAGAAAGACCCTCTCTGGCAGAATCCCTGTGATGACAAACGCCACAGAG
  ACATTTGGTCTAAAGAAAAAACCTGCGATCGCTTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGG
  TACCACTGCCTTGTGTCTGTTCCTGATTATGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAATCC
  TTTGAGGAGGTACAGTTCTTTAATAGAAATAACTACCACAGGGGGATTGATTGGTACATGGATTTCTTCC
  CAGTCCCATCTAATGTCACCACTGACTTTCTGTTTGAGAAGAGTGCTAATTACTTCCATTCAGAGGAAGC
  TCCTAAGAGAGCTGCTTCTCTAGTCCCGAAAGCCAAGATCATCACCATACTCATTGACCCGTCAGACCGA
  GCGTACTCCTGGTACCAGCATCAGCGATCCCACGAAGACCCCGCAGCTCTGAAATTTAGCTTCTATGAAG
  TGATCTCTGCTGGGCCCAATGCACCCTGGGAACTCCGAACCCTGCAGAAGAGATGCCTGGTCCCTGGATG
  GTACGCCAGCCACATCGAGAGATGGCTTGTTTATTTCCCTCCATTTCAGCTGCTAATCATTGACGGGCAG
  CAGCTAAGAACTGCCCCTGCGACAGTGATGGATGAAGTCCAGAAGTTTCTAGGAGTCTCGCCTCATTATA
  ATTACTCCGAAGCTTTAACGTTTGATTCTCATAAAGGCTTCTGGTGTCAGTTGCTGGAAGAAGGGAAAAC
  AAAATGTCTTGGGAAGAGTAAAGGAAGAAAATACCCACCAATGGACTCGGATAGCAGAGCGTTTCTGTCC
  AGCTACTACCGAGACCACAACGTGGAGCTCTCGAAGCTGCTGCACAGACTCGGGCAGCCTCTGCCCTCGT
  GGCTGAGACAGGAGCTGCAGAAAGTGAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_001191716
• Insert Size: 2622 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191716.1, NP_001178645.1
• RefSeq Size: 2776 bp
• RefSeq ORF: 2622 bp
• Locus ID: 295430
• Cytogenetics: 2q42