Stt3b (NM_001170539) Rat Untagged Clone

CAT#: RN217239

Stt3b (untagged) - Rat STT3B, subunit of the oligosaccharyltransferase complex (catalytic) (Stt3b)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Stt3b
• Synonyms: RGD1311563
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217239 representing NM_001170539
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCTCTCAACTCGTCCCCGTGGAGCGGCCTCA
  TGGCTCTGGGGAACAGCCGCCACGGGCACCACGGGCCCGGAGCCCAGAGCGCTTCCAGGGCGGCGGCGGC
  GCCGAAGCCGGGGCCCCCCGCGGGGCTGTCCGGGGGCTTGTCGCAGCCGGCCGGGTGGCAGTCTTTGCTC
  TCCTTCACCATCCTCTTCCTGGCCTGGCTGGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCCGCTTCG
  AGAGCATCATCCACGAGTTCGACCCGTGGTTTAACTATAGATCAACTCATCATCTTGCATCTCATGGGTT
  CTATGAGTTCTTAAATTGGTTTGATGAAAGAGCATGGTACCCACTAGGAAGAATCGTGGGTGGCACCGTT
  TACCCAGGATTGATGATAACAGCTGGCCTTATTCATTGGATTTTAAATACATTGAACATAACAGTTCACA
  TCAGAGATGTATGTGTGTTCCTTGCACCAACTTTTAGCGGCCTTACATCTATATCTACGTTCCTGCTAAC
  TAGAGAACTTTGGAACCAGGGAGCAGGGCTCTTAGCTGCCTGCTTCATTGCTATTGTACCCGGGTACATA
  TCTCGGTCGGTAGCGGGATCCTTTGATAACGAAGGCATTGCGATTTTCGCACTTCAGTTCACTTACTACT
  TATGGGTGAAGTCTGTGAAAACCGGGTCTGTGTTTTGGACAATGTGCTGCTGCTTGTCGTATTTCTACAT
  GGTCTCTGCGTGGGGAGGTTATGTGTTCATCATCAACCTCATCCCTCTCCATGTGTTTGTGCTGCTCCTG
  ATGCAGAGGTACAGCAAGAGAGTCTACATAGCATATAGCACTTTCTACATTGTGGGTTTAATATTATCCA
  TGCAGATACCTTTTGTGGGATTCCAGCCAATCAGGACAAGCGAGCACATGGCAGCTGCAGGTGTCTTTGC
  GCTGCTGCAGGCTTATGCGTTTCTGCAGTATCTGAGAGACCGGTTGACAAAGCAGGAGTTCCAGACCCTT
  TTCTTTTTGGGTGTCTCACTAGCTGCAGGCGCTGTGTTCCTTAGTGTCATCTATCTGACATACACAGGTT
  ACATTGCACCATGGAGTGGCAGGTTTTATTCATTATGGGATACTGGGTATGCAAAAATACACATTCCAAT
  TATTGCATCAGTGTCTGAACATCAGCCTACGACATGGGTGTCTTTCTTCTTTGATCTACATATTCTTGTG
  TGTACCTTCCCAGCAGGCCTATGGTTCTGCATCAAAAATATCAACGATGAAAGAGTATTTGTTGCTCTGT
  ATGCGATCAGTGCTGTCTACTTTGCTGGAGTAATGGTACGGCTGATGCTGACTCTGACCCCAGTCGTCTG
  CATGCTGTCTGCAATTGCCTTCTCCAGTGTTTTTGAGCACTATTTGGGGGATGACATGAAAAGGGAAAAC
  CCACCTGTGGAGGACAGCAGTGACGAGGATGACAGAAGAAGCCCAGGAAACTTGTATGACAAGGCAGGGA
  AAGTGAGAAAGCATGTGACGGAGCAGGAGAAGCCGGAGGAGGGCTTGGGCCCCAACATCAAGAGCATCGT
  GACCATGCTCATGCTCATGCTCCTCATGATGTTCGCTGTGCACTGCACCTGGGTCACCAGCAATGCCTAC
  TCCAGCCCCAGTGTGGTCCTCGCCTCCTACAATCATGATGGAACCAGAAATATCTTAGATGATTTTAGAG
  AAGCATACTTTTGGTTGAGACAAAACACGGATGAACACGCCAGGGTCATGTCCTGGTGGGACTATGGCTA
  TCAGATTGCTGGGATGGCCAACAGGACTACTCTGGTAGACAATAACACCTGGAACAACAGCCACATTGCA
  CTGGTAGGAAAAGCTATGTCTTCTAATGAAACAGCCGCCTATAAAATCATGAGGTCTCTTGACGTTGATT
  ATGTGTTGGTCATTTTTGGAGGAGTTATTGGCTATTCTGGTGATGATATCAACAAGTTCCTCTGGATGGT
  CAGGATAGCTGAAGGGGAGCACCCAAAAGACATTCGGGAAGGCGACTACTTCACCCAGCAGGGAGAGTTC
  CGAGTGGACAGAGCCGGGTCTCCTACTCTGTTAAATTGCCTTATGTATAAAATGTCATACTACAGATTTG
  GAGAAATGCAGCTAGATTTTCGCACACCCCCCGGTTTTGACCGAACACGTAATGCTGAGATCGGAAATAA
  GGACATTAAATTCAAGCATTTGGAGGAAGCCTTTACATCAGAGCACTGGCTTGTCAGGATATATAAAGTG
  AAAGCACCCGACAACAGGGAGACATTAGGTCACAAGCCTCGAGTCACCAACATCTTCCCCAAACAGAAGT
  ATTTGTCAAAGAAGACTACTAAAAGGAAGCGTGGCTACGTTAAAAATAAGCTAGTTTTTAAGAAAGGCAA
  GAAAATGTCTAAGAAGACTGTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170539
• Insert Size: 2475 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170539.1, NP_001164010.1
• RefSeq Size: 2724 bp
• RefSeq ORF: 2475 bp
• Locus ID: 363160
• Cytogenetics: 8q32