Dvl2 (NM_001172056) Rat Untagged Clone

CAT#: RN217153

Dvl2 (untagged) - Rat dishevelled segment polarity protein 2 (Dvl2)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dvl2
• Synonyms: Dvl-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217153 representing NM_001172056
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCAGCAGCGCGGGGGGCGGTGGTGTAGGCGAGACGAAGGTGATTTACCATCTGGATGAAGAAG
  AGACTCCTTACCTGGTGAAGATCCCTGTCCCGGCGGAGCGCATCACGCTCGGCGATTTCAAGAGCGTTTT
  GCAGCGGCCCGCGGGCGCTAAGTACTTTTTCAAGTCTATGGATCAGGATTTTGGGGTGGTGAAGGAAGAG
  ATCTCTGACGACAGTGCCCGCCTACCTTGCTTCAATGGAAGGGTTGTCTCCTGGCTGGTGTCATCAGAGA
  GCCCCCAGCCTGAGACAGCGCCCCCTGCCCATGAGTCTCGGACAGAACTGGTTCCTCCACCTCCACCATT
  ACCCCCTTTGCCACCAGAAAGGACCAGTGGCATTGGGGACTCAAGGCCTCCATCCTTCCACCCTAATGTA
  TCCAGTAGCCATGAAAATCTAGAGCCTGAGACAGAAACTGAATCCGTCGTATCACTGAGACGAGACCGAC
  CTAGGAGGAGAGACAGCAGTGAGCATGGCGCTGGTGGCCACAGGCCTGGCGTCCCCTCAAGGCTGGAGCG
  CCACCTGGCTGGGTATGAGAGCTCTTCCACCCTCATGACCAGCGAACTGGAAAGTACCAGCTTAGGAGAT
  TCAGATGAGGATGACAGCATGAGTAGGTTCAGCAGCTCCACTGAGCAGAGCAGTGCCTCCCGCCTCCTCA
  AGCGCCACCGAAGGCGAAGGAAGCAGCGGCCGCCACGCATGGAGAGGACCTCATCCTTCAGCAGTGTCAC
  AGATTCTACAATGTCTCTCAACATCATCACAGTCACACTCAACATGGAGAAATACAACTTCCTGGGTATC
  TCCATTGTGGGCCAAAGTAACGAGCGTGGTGACGGAGGCATCTATATAGGCTCCATCATGAAAGGGGGAG
  CGGTGGCTGCAGATGGGCGGATCGAGCCTGGAGACATGCTTTTGCAGGTAAACGACATGAACTTTGAGAA
  CATGAGCAATGACGATGCTGTGCGGGTGCTGAGAGACATTGTACACAAGCCAGGCCCCATCGTGCTCACC
  GTGGCCAAGTGTTGGGATCCCTCTCCCCAGGCCTACTTCACCCTCCCCCGAAATGAGCCCATACAGCCAA
  TTGATCCAGCTGCCTGGGTGTCCCATTCAGCTGCGCTAACCGGTGCCTTCCCTGCTTACCCCGGCTCTTC
  ATCTATGAGCACTATCACATCTGGCTCCTCTCTGCCTGATGGCTGTGAAGGCCGGGGTCTCTCTGTCCAC
  ATGGACATGGCCTCTGTGACCAAGGCCATGGCAGCCCCAGAGTCTGGGCTTGAAGTCCGGGACCGCATGT
  GGCTCAAGATCACCATCCCAAACGCCTTTCTAGGCTCGGATGTGGTAGACTGGCTGTATCACCATGTGGA
  AGGCTTTCCTGAGCGCCGGGAGGCCCGCAAGTACGCCAGTGGGCTGTTGAAGGCGGGACTCATCCGGCAC
  ACTGTCAACAAGATTACTTTCTCTGAGCAGTGCTACTATGTTTTCGGGGACCTCAGTGGTGGCTGCGAGA
  GTTACCTAGTTAACCTGTCTCTGAATGACAACGATGGTTCCAGTGGGGCTTCAGACCAGGATACTCTGGC
  TCCTCTTCCTGGAGCTACTCCCTGGCCCCTGCTGCCTACCTTCTCCTACCAATACCCAGCCCCGCACCCC
  TACAGCCCACAGCCTCCGCCCTATCATGAGCTTTCGTCCTACAGCTATGGCGGAGGCAGTGCCAGCAGCC
  AGCACAGTGAGGGGAGCCGGAGCAGTGGGTCAACAAGAAGTGATGGGGGGGCCGGGCGCACAGGGAGGCC
  TGAGGAACGGGCCCCTGAGTCAAAGTCTGGCAGTGGCAGTGAGTCTGAACTCTCCAGCCGGGGAGGCAGC
  CTTCGGCGGGGTGGGGAACCTGGTGGGACTGGTGATGGTGGACCCCCTCCATCCAGGGGCTCAGCAGGTG
  GTACTCCTAACCTCCGAGCTCTCCCAGGCCTCCATCCCTATGGACCTCCACCTGGCATGGCTCTCCCCTA
  TAACCCCATGATGGTAGTCATGATGCCTCCACCCCCACCCCCTGTCTCGACAGCAGTACAGCCCCCTGGT
  GCCCCTCCAGTCAGAGACCTGGGCTCTGTGCCCCCAGAACTGACAGCTAGCCGTCAGAGCTTCCACATGG
  CCATGGGCAACCCCAGTGAGTTTTTTGTAGATGTTATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001172056
• Insert Size: 2211 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001172056.1, NP_001165527.1
• RefSeq Size: 2785 bp
• RefSeq ORF: 2211 bp
• Locus ID: 303251
• Cytogenetics: 10q24