Vcan (NM_001170559) Rat Untagged Clone

CAT#: RN217058

Vcan (untagged) - Rat versican (Vcan), transcript variant 4

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Vcan
• Synonyms: Cspg2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217058 representing NM_001170559
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGATAAATATGAACGGCATCCTATGGATGTGCTCAACCTTACTGTTAACGCATGCACTGCATAAAG
  CCAAAATGGAAGAAAACCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGATCCTACCTTGTCATTTTTC
  AACCTTGCCCACCTTACCACCCGATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAAATAGAA
  GTGGACAAAAATGGAAAAGACATAAAGGAGACTACTGTCCTGGTGGCCCAAGACGGGAACATCAAGATTG
  GTCAGGACTACAAGGGGCGGGTATCAGTGCCTACGCATCCCGATGACGTAGGCGATGCCTCTCTCACCAT
  GGTCAAACTCCGTGCTAGTGACGCAGGTGTCTACCGCTGTGATGTCATGTATGGCATTGAAGACACTCAG
  AACACGATGTCGCTGGCCGTGGACGGTGTCGTGTTTCACTACAGGGCAGCGACCAGCAGATACACTCTGA
  ACTTCGAGTCTGCTCAACAGGCTTGTTTGGACATCGGGGCGGTCATAGCAACCCCAGAGCAGCTGTTCGC
  TGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGACCAAACTGTCAGATATCCCATA
  CGGGCTCCCCGAGAGGGCTGTTATGGAGACATGATGGGGAAGGAAGGGGTCCGGACCTATGGATTCCGCT
  CTCCCCAGGAAACCTATGATGTGTATTGCTATGTGGATCATCTGGACGGCGATGTGTTCCACATCACTGC
  TCCCAGTAAATTCACCTTCGAGGAGGCCGAAGCAGAGTGTGCAAACCGGGATGCCAGGCTGGCGACTGTT
  GGGGAACTTCACGCAGCTTGGAGGAACGGCTTTGACCAGTGCGATTACGGCTGGCTGTCGGATGCCAGCG
  TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACCCTGTATCG
  TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACGACCTGAT
  CTCTGCAAAACAAACCCATGCCTCAATGGAGGCACCTGCTATCCTACTGAGACTTCCTATGTGTGCACCT
  GTGCACCTGGCTACAGTGGAGACCAGTGTGAACTGGATTTTGATGAATGTCACTCTAACCCTTGTCGGAA
  TGGAGCCACCTGTGTGGACGGTCTGAATACATTTAGATGCCTCTGCCTTCCGAGTTATGTCGGTGCACTC
  TGCGAACAAGACACTGAGACATGCGACTATGGCTGGCACAAATTCCAAGGGCAATGCTACAAGTACTTTG
  CTCATCGCCGTACATGGGATGCTGCTGAAAGGGAGTGTCGCCTGCAGGGTGCCCACCTCACAAGCATCCT
  TTCTCATGAGGAACAAATGTTTGTGAATCGTGTGGGCCATGATTACCAGTGGATTGGCCTCAATGACAAG
  ATGTTTGAACATGACTTCCGCTGGACTGACGGCAGCGCACTGCAATATGAGAACTGGAGACCCAACCAGC
  CAGACAGCTTCTTTTCTGCTGGAGAAGACTGCGTTGTGATCATTTGGCATGAGAATGGCCAGTGGAATGA
  CGTCCCCTGCAACTACCACCTCACCTACACCTGCAAGAAGGGAACAGTTGCTTGCGGCCAACCCCCTGTT
  GTAGAAAATGCCAAGACCTTTGGAAAGATGAAACCACGTTATGAAATCAACTCCTTGATTAGATACCACT
  GCAAAGATGGTTTCATTCAGCGTCACCTTCCAACTATCCGGTGCCTAGGAAATGGGAGATGGGCAATGCC
  TAAAATAACCTGCATGAACCCATCTGCATACCAAAGGACTTATTCTAAGAAATACTTAAAAAATTCCTCA
  TCAGTCAAGGACAATTCTATAAATACGTCAAAACATGAGCATCGCTGGAGCCGGAGGTGGCAGGAAACGA
  GGCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170559
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170559.1, NP_001164030.1
• RefSeq Size: 4175 bp
• RefSeq ORF: 1968 bp
• Locus ID: 114122
• UniProt ID: Q9ERB4
• Cytogenetics: 2q12
• Gene Summary: extracellular matrix protein secreted by many cell types; part of the large chondroitin sulfate proteoglycan (CSPG) family [RGD, Feb 2006]
  Transcript Variant: This variant (4) lacks two alternate in-frame exons, compared to variant 1, resulting in a shorter protein (isoform 4, also referred to as V3), compared to isoform 1.