Parn (NM_001270415) Rat Untagged Clone

CAT#: RN217019

Parn (untagged) - Rat poly(A)-specific ribonuclease (Parn)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Parn
• Synonyms: RGD1565449
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217019 representing NM_001270415
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATAATCCGGAGCAATTTTAAGATTAATCTTCCCAAAGTGTACCAGGCCATAGAGGAGGCTGACT
  TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGTGATGGACCCTCAGTAACAGCATTAACAAGTGGTTT
  TGACACCCCAGAAGAGAGATATCAGAAGCTTAAAAAGCATTCCATGGACTTCTTGCTGTTTCAGTTTGGC
  CTCTGTGCTTTTAAGTATGACCACGCCAATTCCAAGCATGTAACGAAGTCGTTTAACTTCTATGTTTTCC
  CCAAGCCTTTCAGTAGGTCCTCACCAGATGTCAAGTTTGTTTGCCAGAGCTCCAGCATTGACTTTCTGGC
  AAGCCAAGGATTTGACTTTAATAAAGTGTTTTGCAGTGGAATTCCATATCTGAACCAGGAAGAAGAAAGA
  CAGCTGAGAGAGCAATTTGATGAAAAACGTTCTCAGGCCAATGGGGCCGGAGCTCTGACAAAATGTCCTG
  TGACAATTCCTGAGGATCAGAAGAAGTTTATTGACCAAGTAATAGAGAAGATAGAGGGTTTCCTACAGAG
  AGAAGAGAAGAGGACTCTGGAACTTAACCCATGCACTGGGTTCCAAAGAAAACTTATTTATCAGACTTTG
  AGTTGGAAGTATCCCAAAGGCATTCATGTTGAGACATTAGAGACTGACAAGAAGGAAAGACACATAGTTA
  TCAGCAAAGTGGATGAAGAAGAACGCAAAAGGAGAGAACAGGAGAAGTACGCAAAGGAGCAGGAGGAACT
  GAATGATGCTGTAGGATTTTCAAGAGTCATCCATGCGATTGCTAATTCGGGGAAGCTGGTTGTTGGACAC
  AACATGCTCTTGGATGTCATGCACACTATTCACCAGTTCTACTGCCCTCTGCCTGTGGACCTGAATGAGT
  TTAAGGAGATGACAACATGTGTCTTCCCCAGACTCTTGGATACTAAATTGATGGCCAGCACCCAGCCCTT
  TAAGGATATCATTAACAATACATCCCTTGCAGAGTTGGAAAAGCGGTTGAAAGAGACACCTTTTGACCCT
  CCTAAAGTTGAGAGTGCGGAAGGCTTTCCAAGCTACGACACAGCTTCTGAGCAGCTGCATGAGGCAGGTT
  ATGATGCGTACATCACAGGACTCTGCTTCATCTCCATGGCAAATTACTTAGGTTCTTTACTCAGTCCTCC
  AAAAACGTGTGTGTCCGCCAGATCAAAGCTCATCGAACCCTTTTTTAACAAGTTATTTCTTATGAGGGTC
  ATGGATATTCCCTATTTAAACTTGGAAGGGCCAGACTTGCAGCCTAAGCGGGATCATGTTCTCCATGTGA
  CCTTCCCTAAAGAATGGAAAACCAGTGACCTGTACCAGCTCTTCAGCGCCTTTGGTAACATTCAGATATC
  CTGGATTGATGACACATCAGCCTTCGTTTCTCTCAGCCAACCAGAACAAGTACAAATTGCTGTTAATACC
  AGTAAGTATGCTGAAAGTTATCGGATCCAGACCTACGCTGAGTATGTGGGAAAGAAGCAGGAAAGCAAGC
  AGGTCAAGAGGAAGTGGACAGAAGACAGTTGGAAGGAGGTGGACAGAAAGCGGCCCCACGTGCCGGGCCC
  CTGTTACCACACCAACAGCTTCACTGCAGCCGGCATAGTTGGAAAGAGAACTCTGAGTCCCGACCCTCGG
  GAAGCTGGAGTGGTGGACAGAGAATCAGAGGGAATATCTGACCCGGAACTTGAACAGACGGATTACTGTG
  CAGACCCCCTCCCAGAGGGAAGGAAAAAGGCCAAGAAGTTAAAAAGAATGAAGAAAGAGTTTTCCTTGGC
  AGGAAGTGCCTCAGATAGCCCTGCTGTGCTTTTTGAAGTCCCCGACACGTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270415
• Insert Size: 1875 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270415.1, NP_001257344.1
• RefSeq Size: 1875 bp
• RefSeq ORF: 1875 bp
• Locus ID: 360464
• Cytogenetics: 10q11