Erf (NM_001170335) Rat Untagged Clone

CAT#: RN216897

Erf (untagged) - Rat Ets2 repressor factor (Erf)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Erf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216897 representing NM_001170335
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACCCCAGCGGACACAGGGTTTGCCTTCCCAGATTGGGCCTACAAGCCGGAGTCATCCCCTGGCT
  CGAGGCAGATCCAGCTGTGGCACTTTATCTTGGAGCTGCTTCGGAAAGAGGAGTACCAGGGCGTCATCGC
  CTGGCAGGGAGACTACGGGGAATTTGTCATCAAGGACCCTGATGAGGTGGCCCGACTCTGGGGGGTCCGC
  AAGTGCAAACCCCAGATGAACTACGACAAGCTGAGCCGGGCCCTGCGCTATTACTATAACAAGCGCATTC
  TGCACAAGACCAAGGGGAAACGGTTCACCTACAAGTTCAACTTCAACAAACTGGTGCTGGTTAATTACCC
  TTTCATCGATGTGGGGCTGGCCGGGGGCGCTGTTCCCCAGAGTGCCCCTCCAGTGCCATCGGGCGGCAGC
  CATTTCCGCTTCCCTCCTTCGACGCCCTCCGAGGTGCTGTCCCCCACCGAGGATCCCCGCTCTCCACCAG
  CTTGTTCTTCATCGTCCTCTTCTCTCTTCTCCGCTGTGGTTGCTCGACGCCTGGGCCGAGGCTCTGTCAG
  CGACTGCAGTGATGGCACCTCAGAGCTGGAGGAGCCTCTGGGGGAGGACCCCAGGGCACGACCACCTGGC
  CCTCCGGAGCTGGGTGCCTTCCGAGGGCCCCCGCTGGCCCGCCTCCCACATGATCCTGGTGTCTTCCGTG
  TCTATCCTCGGCCTCGGGGTGGTCCTGAACCCCTAAGTCCCTTCCCTGTGTCACCTTTGGCTGGGCCTGG
  CTCCCTTCTACCCCCTCAACTCTCCCCAGCTCTGCCCATGACTCCCACCCACCTGGCCTACACACCCTCA
  CCCACGCTGAGTCCTATGTACCCCAGTGGTGGCGGGGGCCCTAGTGGCTCAGGGGGGGGTTCCCACTTCT
  CCTTCAGTCCGGAGGACATGAAACGGTACCTGCAGGCCCACACCCAAAGCGTCTACAACTACCACCTCAG
  TCCCCGCGCCTTCCTGCACTATCCAGGGCTGGTGGTGCCCCAGCCTCAGCGCCCTGACAAGTGCCCACTG
  CCGCCCATGGCACCGGAGACCCCGCCGGTTCCCTCCTCAGCCTCGTCTTCCTCTTCTTCCTCTTCATCCC
  CGTTCAAGTTTAAGCTGCAGCCACCCCCGCTAGGACGCCGGCAGCGGGCAGCTGGAGAGAAGGCTCCAGG
  GGGCACTGACAAGAGCAGTGATGGTAGTGGCTCTGGTGGGCTGGCCGAGGGGGCAGGCGCATTAGCCCCC
  CCACCGCCACCACCACCCCAGATTAAGGTGGAACCCATCTCAGAGGGCGAGTCGGAGGAGGTGGAGGTGA
  CTGACATCAGTGACGAAGATGAGGAAGATGGGGAGGTGTTCAAGACCCCCCGTGCCCCACCCGCTCCCCC
  CAAGCCAGAGCCTGGAGAGGCACCAGGGGCAGCCCAGTGCATGCCTCTTAAACTGCGCTTTAAAAGGCGC
  TGGAGTGAAGACTGTCGCCTGGAGGGGGGCGGGTGCCTCTCTGGGGGCCCTGAGGATGAGGGTGAGGACA
  AGAAGGTGCGTGGGGACATGGGCCCTGGGGAGCCTGGGGGACCCCTCACTCCTCGGCGAGTGAGCTCTGA
  TCTCCAGCACGCTACAGCCCAGCTCTCCCTGGAGCACCGAGATTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170335
• Insert Size: 1659 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170335.1, NP_001163806.1
• RefSeq Size: 3711 bp
• RefSeq ORF: 1659 bp
• Locus ID: 292721
• Cytogenetics: 1q21