Etv1 (NM_001163156) Rat Untagged Clone

CAT#: RN216758

Etv1 (untagged) - Rat ets variant 1 (Etv1), transcript variant 1

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Etv1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216758 representing NM_001163156
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGATTTTATGACCAGCAAGTGCCTTACGTGGTCACCAACAGTCAGCGTGGGAGAAACTGTACCG
  AGAAACCAACAAATGTCAGGAAAAGAAAATTCATTAACAGAGATCTGGCTCATGATTCAGAAGAACTCTT
  TCAAGATCTGAGTCAGTTACAAGAAACATGGCTTGCTGAAGCTCAGGTACCTGACAATGATGAGCAGTTT
  GTGCCAGACTATCAGGCCGAAAGTTTGGCTTTTCATGGCCTACCACTGAAAATCAAGAAAGAACCCCACA
  GCCCATGTTCAGAACTCGGGTCTGCTTGCAGCCAAGAGCAGCCCTTTAAATTCAGCTATGGAGAAAAGTG
  CCTGTACAATGTCAGTGCCTATGATCAGAAGCCACAAGTGGGAATGAGGCCCTCCAATCCCCCAACACCA
  TCCAGCACTCCAGTGTCCCCACTACATCATGCATCTCCAAACACAGCTCATACTCCGAAACCTGACCGGG
  CCTTCCCAGCTCACCTCCCGCCTTCCCAGTCCATACCAGACAGCACCTACCCCATGGACCACAGATTTCG
  CCGCCAGCTTTCTGAACCCTGTAACTCTTTCCCTCCTTTGCCAACAATGCCAAGGGAAGGGCGTCCTATG
  TACCAACGCCAGATGTCTGAGCCAAACATCCCCTTCCCACCACAAGGCTTCAAGCAGGAGTACCACGACC
  CAGTCTATGAACATACCACCATGGTTGGCGGTGCAGCCAGCCAAAGCTTTCCCCCTCCTTTGATGATCAA
  ACAGGAACCCAGAGACTTTGCATATGATTCAGAAGTGCCTAGCTGCCACTCCATTTATATGAGGCAAGAA
  AGCTTCCTGGCTCATCCAAGCAGAACAGAAGGCTGCATGTTTGAGAAGGGTCCCAGGCAGTTCTATGATG
  ACACCTGTGTCGTCCCGGAGAAGTTCGATGGGGACATTAAGCAAGAGCCAGGAATGTACCGGGAAGGTCC
  CACGTACCAGAGGCGAGGATCGCTTCAGCTCTGGCAGTTTCTGGTAGCTCTTCTGGATGACCCTTCAAAT
  TCTCATTTTATTGCCTGGACTGGGCGAGGCATGGAATTTAAACTGATTGAGCCTGAAGAGGTGGCCCGAC
  GTTGGGGCATTCAGAAGAACAGGCCAGCTATGAACTATGACAAACTTAGCCGTTCTCTCCGCTATTATTA
  TGAGAAGGGAATCATGCAAAAGGTGGCTGGAGAAAGATATGTCTACAAATTTGTGTGTGACCCGGAAGCC
  CTTTTCTCCATGGCCTTTCCGGATAATCAGCGCCCCCTGCTGAAGACGGACATGGAACGTCACATCAACG
  AAGAGGACACGGTGCCCCTGTCTCACTTTGATGAGAGCATGACCTACATGCCAGAAGGGGGCTGCTGCAA
  CCCCCACCCTTACAACGAAGGATACGTGTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163156
• Insert Size: 1434 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163156.3, NP_001156628.1
• RefSeq Size: 6792 bp
• RefSeq ORF: 1434 bp
• Locus ID: 362733
• Cytogenetics: 6q21