Smyd2 (NM_206851) Rat Untagged Clone

CAT#: RN216661

Smyd2 (untagged) - Rat SET and MYND domain containing 2 (Smyd2)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Smyd2
• Synonyms: HSKM-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216661 representing NM_206851
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCCGAGGCCCGCGGCGGCCTGGAGCGCTTCTGCAGCGCGGGCAAGGGCCGGGGGCTCCGTGCGC
  TGCGGCCCTTCCACGTGGGCGACCTCCTCTTCTCCTGCCCGGCCTACGCCTGCGTGCTCACCGTGGGCGA
  GCGGGGCCACCACTGCGAGTGCTGCTTCGCCAGGAAAGAAGGATTGTCGAAATGTGGACGATGCAAGCAA
  GCCTTCTACTGCGATGTGGAGTGTCAGAAAGAAGACTGGCCCCTGCACAAGCTGGAGTGCTCCTCCATGG
  TTGTTTTCGGGGAGAACTGGAATCCCTCGGAGACTGTGCGGCTCACAGCAAGGATCCTGGCCAAGCAGAA
  AATGCACCCAGAGAGGACACCTTCAGAGAAACTGTTGGCCGTGAGGGAGTTTGAGTCACATCTGGACAAG
  CTAGACAACGAGAAGAAGGATCTCATCCAGAGCGACATCGCGGCGCTCCATCAGTTCTACTCCAAGCACC
  TGGAGTTCCCTGACCACAGCAGCCTTGTGGTGCTCTTTGCCCAGGTGAACTGTAATGGCTTCACTATTGA
  AGATGAGGAGCTCTCTCACTTGGGATCGGCGATATTTCCTGATGTTGCGCTGATGAATCACAGCTGCTGC
  CCGAATGTCATTGTGACCTACAAAGGTACCCTGGCAGAAGTCAGAGCTGTGCAGGAGATCCACCCAGGAG
  ATGAGGTGTTCACCAGCTATATCGACCTGCTGTATCCAACAGAAGACAGGAACGACCGGTTAAGAGACTC
  CTACTTCTTTACCTGTGAGTGCCGGGAGTGTACGACCAAGGACAAGGACAAGGCCAAGGTGGAAATCCGG
  AAGCTCAGCAACCCACCTCAGGCAGAAGCCATCCGAGACATGGTCAGATACGCACGCAATGTCATCGAGG
  AGTTCCGGAGGGCCAAGCACTACAAATCCCCTAGTGAGCTGTTGGAAATCTGTGAGCTCAGCCAGGAGAA
  GATGAGCTCTGTGTTTGAGGACAGCAATGTGTACATGCTACACATGATGTACCAGGCCATGGGCGTCTGC
  CTGTACATGCAGGACTGGGAAGGAGCCCTGAAATATGGCCAGAAGATCATCAAACCCTACAGTAAGCACT
  ACCCCGTGTACTCCCTCAACGTGGCCTCCATGTGGCTGAAGTTGGGAAGACTGTACATGGGCCTGGAGAA
  CAAAGCTGCCGGGGAGAAGGCCCTGAAGAAGGCCATCGCCATCATGGAAATAGCTCATGGCAAGGACCAC
  CCGTACATCTCCGAGATCAAGCAGGAAATTGAGAGCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_206851
• Insert Size: 1302 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206851.1, NP_996733.1
• RefSeq Size: 1592 bp
• RefSeq ORF: 1302 bp
• Locus ID: 289372
• UniProt ID: Q7M6Z3
• Cytogenetics: 13q27
• Gene Summary: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860' (By similarity).[UniProtKB/Swiss-Prot Function]