Mxra8 (NM_001007002) Rat Untagged Clone

CAT#: RN215685

Mxra8 (untagged ORF) - Rat matrix-remodelling associated 8 (Mxra8), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mxra8
• Synonyms: 1200013a08rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN215685 representing NM_001007002
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGAACTGCTATCCCGTGTCTTGTTGTGGAAACTGGTGCTTCTTCAGAGTTCTGCAGTCCTGTCCTCAG
  GGTCTCCAGGGACCGCAGCAGCCAGCAGCTCTGTGGTGTCTGAGTCTGCGGTGAGCTGGGCAGCCGGAAC
  TCAGGCGGTGCTACGCTGCCAGAGCCCGCGCATGGTGTGGACCCAAGACCGGCTGCACGATCGCCAGCGC
  GTGGTCCACTGGGACCTCAGCGGTGGCCCAGGTAGCCAAGGGCGCCGACTTGTGGATATGTACTCGGCCG
  GGGAACAGCGCGTGTACCAGCCGCGCGATCGCGACCGCCTCCTGCTGTCGCCTTCTGCCTTCCACGACGG
  CAACTTCTCGCTGCTCATCCGCGCTGTGGAGAGAGGCGACGAAGGGGTGTACACCTGCAATCTGCACCAT
  CACTACTGCCACCTCTACGAGAGCCTGGCTGTGCGCCTCGAGGTCACTGACGATCCCCTATTAAGTCGCG
  CATACTGGGACGGAGAGAAGGAAGTGTTGGTGGTGGCCCTCGGCGCACCGGCTCTGATGACTTGCGTGAA
  CCGTGAGCACTTGTGGACTGACCGCCACTTAGAGGAGGCGCAGCAGGTGGTCCATTGGGACCGACAGCTA
  CCTGGCGTGCCACATGACCGCGCTGACCGATTGCTTGACCTGTACGCATCCGGCGAGCGCCGAGCCTACG
  GGCCACCCTTCCTGCGTGATCGCGTGTCGGTGAACACCAACGCTTTTGCACGCGGGGACTTCTCCCTACG
  CATAGATGACCTGGAGCCGGCTGATGAGGGCATCTATTCCTGCCACCTGCACCACCACTACTGTGGTCTC
  CATGAGCGCCGAGTCTTCCACCTACGGGTCACGGAGCCTGTTTTTGAGCCACCAGCTCGCGCTTCTCCTG
  GCAATGGGTCTGGTCACAACAGTGTTCCTAGCCCAGATCCCACCATGGCCCGTGGCCACAGCATCATCAA
  CGTCATTGTCCCAGAGGACCACACACATTTCTTCCAGCAATTGGGCTATGTGCTGGCCACTCTACTGCTT
  TTCATCTTGCTGCTCATCACTGTAGTCCTGGCGACACGCCACCGTCACAGTGGAGGATGCAAGACCTCAG
  ACAGGAAAGCTGGGAAATCAAAGGGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_001007002
• Insert Size: 1149 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007002.1, NP_001007003.1
• RefSeq Size: 2296 bp
• RefSeq ORF: 1149 bp
• Locus ID: 313770
• UniProt ID: Q5XI43
• Cytogenetics: 5q36
• Gene Summary: Transmembrane protein which can modulate activity of various signaling pathways, probably via binding to integrin ITGAV:ITGB3. Mediates heterophilic cell-cell interactions in vitro. Inhibits osteoclastogenesis downstream of TNFSF11/RANKL and CSF1, where it may function by attenuating signaling via integrin ITGB3 and MAP kinase p38. Plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes. Stimulates formation of primary cilia in chondrocytes. Enhances expression of genes involved in the hedgehog signaling pathway in chondrocytes, including the hedgehog signaling molecule IHH; may also promote signaling via the PTHLH/PTHrP pathway. Plays a role in angiogenesis where it suppresses migration of endothelial cells and also promotes their apoptosis. Inhibits VEGF-induced activation of AKT and p38 MAP kinase in endothelial cells. Also inhibits VTN (vitronectin)-mediated integrin ITGAV:ITGB3 signaling and activation of PTK2/FAK. May play a role in the maturation and maintenance of the blood-brain barrier.[UniProtKB/Swiss-Prot Function]