Rec8 (NM_001011916) Rat Untagged Clone

CAT#: RN215104

Rec8 (untagged ORF) - Rat REC8 homolog (yeast) (Rec8), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Rec8
• Synonyms: Rec8L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN215104 representing NM_001011916
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTACTATCCCAACGTGCTTCAGCGCCACACAGGCTGCTTCGCCACTATCTGGCTGGCCGCTACCC
  GGGGCAGCCGTTTGGTGAAGCGGGAATACCTGAAAGTGAACGTGGTAAAGACCTGCGAGGAAATCCTCAA
  TTATGTGCTCGTACGAGTGCAACCTCCGATGCCTGGGCTGCCCCGCCCCCGCTTCTCCCTCTATCTCTCC
  GCCCAGCTTCAGATTGGTGTGATAAGGGTCTACTTTCAACAGTGCCAGTACCTTGTGGAAGACATCCAGC
  ATATCCTAGAGCACCTACACCGGGCCCAGCTGCGGATTCGCATTGATATGGAGGAGGCTGACTTACCTAG
  CTTGCTTCTTCCCAATTGCCTGGCCATGATGGAGACGCTGGAGGACGCCCCAGAACCCTTTTTTGGGACG
  ATGTCTGTGGACCCTACACTTCCCAGTCCTTTTGATATTCCTCAGATTCGACACCTTTTAGAGGCTGCAA
  CCCCGGAGAAAATTCGTGAGGAGACCCTTCCTGAAGCCACGCCGGAGCCCAGGAAGCCTGACAGGACCTT
  GGCCATGGTACAGTCCCCTGAGGTCATCACCTTGCAGGAGGCAGAGCCCATCCGAATGCTGCGGATTGAG
  GGAGAGCAGGATCTCCCAGAGATCAGCAGAGGAGACTTAGACCTGCTGATCGCAGAGGAAGATGACGCCA
  TATTGCTGGAGGAACGTCAGAGAGGCAGACTTCTCCGGCAGCGGAGGGCTTCCCCACCACTGGATGAATC
  CAAGGAAGAGCCCCGGGCCCTGGAAGGAGATGGTTTGGTCTCCTCACTCTCACCTCCAGCTCCTGCACAG
  GTTGAAGGGATAAGAGAACCACTTCCAGACCAGGCCTTTCCCCCTGAGGTACAGAAGATGCCAGGCTGGG
  AGCCTGGGGTCCTACTCACTGAGGTGACCCCACCTCAGGAGCTGCGTCTGCCTGCCCCACCGAGTATAGA
  GAAGAGGCCCCCGTCTCCTCAGAGGCCACCTCGCCGGCGCCGCCGGGGGCGCCAGTTATTGTTCTGGGAC
  AAGGAGACCCAGATCTCCCGGGAGAAGTTTGAGGAACAGCTGCAGACTGGGGCTCACTGCTGCGAGTATC
  CAGTGGTCCAGCCTCCCAAGAGGAAGCTCATGAGCCCAGCGGAGCTCTTCAGAACCCCAACTCTCTCTGG
  CTGGTTATCTCCAGAACTACTAGCTTTATGGACCCATTGTGCCCAGGTTCCCCCAAGAATGCTCAGACAA
  AGGCCACAGCTGGAGACTGAAGAGACAGTTGAGGAAGAGAGAGTGGCAGACAAGGAAGAAAGGAGAAAGA
  CTGAAGCTCTGAGTGAGATTGAGGTTCTGAGAGAGGCCCAGGAGCCCAGTGGTCCCCTCATGTTGTCTTC
  AGAGCTCTCCCTAGAAGTAGCAGAAGAAGAAAAATCTCGAACTAGCTTCATCCCTCCAGAAGAACGGTGG
  GCCTGGATTGAGGAGAGGCAGCCAGAGCCCCCTGCACTGCCCATGCTGCCTGAACTTCCTGAAGTGCCCA
  TGGAGATGCCCCCAGGGCCTGAACTACTCTCTTCAGAGGCTGTGCTTAGGGCAGTGGCTCTGGAGCTGCA
  GGCTAACAGGGAGCCTGACTTCAGCAGCCTGGTGCCCCCTCTCAGCTCCCGAAAGTTGGCTTCTCGGGTC
  TTCTATCTGCTCTTGGTGCTGTCCGCACAGAAGATCCTTCTTGTGGACCAACAGAAGCCATATGGGCGCC
  TCCTGATTCGTCTAGGACCCAAATTCCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001011916
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011916.1, NP_001011916.1
• RefSeq Size: 2049 bp
• RefSeq ORF: 1782 bp
• Locus ID: 290227
• UniProt ID: Q6AYJ4
• Cytogenetics: 15p13
• Gene Summary: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).[UniProtKB/Swiss-Prot Function]