Plk1 (NM_017100) Rat Untagged Clone

CAT#: RN214157

Plk1 (untagged ORF) - Rat polo-like kinase 1 (Drosophila) (Plk1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Plk1
• Synonyms: Plk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214157 representing NM_017100
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCAGCGGCCAAAGCTGGAAAGCTGGCTCGAGCACCAGCCGACCTCGGGAAAGGTGGGGTTCCGG
  GAGATGCAGTTCCCGGTGCCCCAGTGGCCGCCCCGCTGGCGAAAGAAATTCCGGAGGTCCTAGTGGACCC
  ACGCAGCCGGCAGCAGTATGTACGGGGCCGCTTCCTGGGTAAAGGAGGCTTCGCCAAATGCTTCGAAATC
  TCAGACTCAGACACAAAAGAGGTGTTCCCAGGCAAGATCGTGCCTAAGTCTTTGCTGCTCAAGCCCCATC
  AGAAGGAGAAAATGTCTATGGAGACCTCCATTCACCGCAGCCTCGAACACCAGCACGTCGTAGGCTTCCA
  TGGCTTTTTTGAGGACAGCGACTTTGTGTTCGTGGTTTTGGAGCTCTGTCGCAGGAGGTCTCTCCTGGAG
  CTGCACAAAAGGAGGAAGGCGCTGACCGAGCCCGAGGCCCGATACTACCTGCGGCAGATAGTCCTGGGTT
  GCCAGTACCTGCACCGCAATCAAGTCATTCACAGGGACCTCAAACTGGGCAACCTCTTTCTGAATGAGGA
  TCTGGAGGTGAAAATAGGGGATTTTGGCCTGGCAACAAAAGTGGAATATGAAGGGGAACGGAAGAAGACC
  CTGTGTGGCACTCCTAACTACATAGCTCCCGAGGTGCTGAGCAAGAAGGGACACAGCTTTGAGGTGGACG
  TGTGGTCCATTGGGTGCATCATGTATACCTTGCTGGTGGGCAAGCCACCTTTTGAGACCTCATGCCTAAA
  AGAGACCTACCTCCGGATCAAGAAAAATGAATACAGTATTCCCAAGCACATCAACCCAGTGGCTGCCTCC
  CTCATCCAGAAAATGCTTCAGACAGACCCCGCTGCGCGCCCCACCATTCACGAGTTGCTCAATGACGAGT
  TCTTCACTTCCGGATACATCCCCGCCCGTCTGCCTATTACCTGCCTCACCATCCCACCAAGGTTTTCAAT
  AGCTCCCAGCAGCCTAGACCCCAGCAACAGGAAGCCTCTCACAGTCCTTAATAAAGGTGTGGAGAACCCC
  CTGCCTGACCGTCCCCGAGAGAAAGAGGAACCAGTGGTTCGGGAGACAAATGAGGCCATCGAGTGCCACC
  TTAGTGACTTGCTACAGCAGCTGACCAGTGTCAACGCCTCCAAGCCCTCAGAGCGAGGGCTGGTGCGGCA
  AGAGGAGGCCGAGGATCCTGCTTGCATCCCTATCTTCTGGGTCAGCAAATGGGTAGACTATTCGGACAAG
  TATGGCCTTGGTTATCAGCTATGTGACAACAGTGTGGGAGTGCTCTTTAATGACTCAACACGCCTCATCC
  TCTACAATGACGGGGACAGCCTGCAGTACATAGAGCGTGATGGCACGGAGTCCTACCTCACTGTGAGCTC
  CCACCCCAACTCCTTGATGAAGAAGATCACTCTCCTCAACTATTTCCGCAATTACATGAGTGAACACCTG
  CTGAAGGCGGGGGCCAACATCACGCCCCGGGAAGGCGACGAGCTGGCCCGGCTGCCCTACCTACGAACAT
  GGTTCCGCACACGCAGCGCCATCATCCTGCACCTCAGCAATGGCACAGTACAGATTAATTTCTTCCAGGA
  CCACACCAAACTTATCCGTGGCCCCCTGATGGCAGCAGTGACCTACATCAACGAGAAGAGGGACTTCCGG
  ACATACCGCCTGAGCCTTCTGGAGGAATATGGCTGCTGCAAGGAACTGGCTAGCCGCCTCCGCTATGCCC
  GCACCATGGTAGACAAGCTGCTGAGCTCGCGTTCCGCCTGCAACCGCCTCAAGGCCTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017100
• Insert Size: 1812 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017100.1, NP_058796.1
• RefSeq Size: 2198 bp
• RefSeq ORF: 1812 bp
• Locus ID: 25515
• UniProt ID: Q62673
• Cytogenetics: 1q36
• Gene Summary: human homolog catalyzes the phosphorylation of a Golgi reassembly stacking protein (GRASP65); may play a role in Golgi apparatus fragmentation and reorganization during mitosis [RGD, Feb 2006]