Dis3l (NM_001008380) Rat Untagged Clone

CAT#: RN213833

Dis3l (untagged ORF) - Rat DIS3 mitotic control homolog (S. cerevisiae)-like (Dis3l), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dis3l
• Synonyms: RGD1308959
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213833 representing NM_001008380
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAACGGCTTGTCAAGCTGTGCAGCATCAGAGAGGCCGGAGACAGTATAACAAACTGCGAAACCTCC
  TGAAGGATGCTCGCCATGACTGCGTTCTCTTTGCTAACGAGTTCCAGCAGCACTGTTACCTCCCCCGGGA
  AAAGGGGGAAGCCATGGAGAAGTGGCAGACCAGGAGCATATACAACTCAGCTGTGTGGTACTACCACCAC
  TGCGAGGACAGGATGCCCATCGTTATGGTGACAGAAGACGAAGAGGCCATTCAGCAGTATGGAAGCGAAA
  CAGAAGGCGTATTTGTAATTTCTTTCAAGAATTACCTGGACAACTTCTGGCCAGATTTAAAGGCTGCCCA
  TGAGCTGTGTGATTCCATCATTCAGTCTCGCCGGGAAAGGGAGAGCGAGAGTCAGGAAACCCATGGGAAA
  GAGTACCCGGAGCACCTTCCCCTGGAAGTGCTGGAAGCAGGCATCAAATCTGGACGCTACATCCAGGGAA
  TTCTGAACGTCAACAAGCACAGAGCTCAAATAGAGGCTTTTGTCCGCCTACAAGGAGCCAGCAGCAAGGA
  CTCAGGCTTGGTCAGCGACATCCTTATCCATGGCTCAAAGGCTCGGAACCGCTCCATCCACGGAGATGTG
  GTGGTGGTGGAGCTGCTCCCCAAAAGTGAGTGGAAAGGAAGAACTGCTGCCCTGTGTGAGAATGACAGTG
  AAGACAAGGCCTCGGGCGAGTCTCCGAGTGAGCCCATGCCCACAGGTCGAGTGGTAGGCATCCTTCAAAA
  GAACTGGCGAGATTATGTGGTGACATTTCCATCCAAAGAAGAGGTCCAGTCTCAGGGCAAAAATGCTCAG
  AAGATCCTAGTTACGCCGTGGGATTACAGAATCCCCAAAATCCGCATCAGCACCCAGCAAGCAGAGGCCC
  TCCAGGATTTCAGGGTGGTTGTGCGCATTGATTCCTGGGAGACAACATCGGTGTATCCAAATGGACACTT
  TGTGCGCGTCTTAGGGAGAATCGGTGATCTGGAAGGGGAGATTGCGACCATCCTGGTAGAGAACAGTATT
  AACGTCGTCCCCTTCTCGGAAGCCCAGATGTGTGAGATGCCAGTGAACACAGCAGAAAACCCTTGGAAAG
  TGAGTCCCAAAGAAGAGCAGGAGCGGAGGGACCTGAGGAGCACCCACCTTGTGTTCAGCATCGACCCTAA
  AGGTTGTGAGGACGTGGATGACGCGCTCTCAGTCAGAACCTTGAATAACGGCAACCTGGAGCTCGGGGTC
  CACATAGCTGATGTCACCCACTTTGTGGCCCCCAACTCTTACATCGATGTTGAAGCTAGAACGAGGGCCA
  CCACTTACTACCTAGCCGACCGCCGCTACGACATGCTGCCTTCCATCCTCAGCGCAGACCTCTGCTCCCT
  CCTGGGAGGCGTTGACCGGTATGCTGTGTCAGTCATGTGGGAATTAGATAAAACCTCTTATGAAATTAAG
  AAGGTGTGGTACGGCAGAACCATTATCCGATCAGCTTACAAACTGTTCTATGAGGCGGCCCAGGAACTTC
  TGGATGGAAACTTCAGCATTGTTGATGATATTCCAGAATTCAAAACCTTGGAGGAGCAGAACCGACAGGC
  CAAACTGGAGGAGTTAGTATGGGCAATTGGGAAGTTGACTGACATAGCTCGCCACATCCGAGCAAAGCGA
  GACCGCTGCGGCGCCTTGGAGCTAGAAGGTGTGGAGGTTCGAGTCCAGCTGGATGACAAGAAGAACATTC
  ATGACCTCATCCCCAAGCAGCCCCTGGAGGTTCATGAGACGGTGGCTGAATGCATGATCCTGGCCAACCA
  CTGGGTGGCCAAGAAGATCTGGGAGAGCTTCCCCCACCAGGCGCTGCTGCGCCAACACCCTCCTCCACAC
  CAGGAGTTTTTCTCAGAGCTCCGGGAATGTGCAAAAGCAAAAGGCTTCTTCATAGACACACGGTCCAATA
  AAACCCTGGCTGACTCTCTGGATAGTGCAAATGACCCCAGTGACCCCCTGGTAAACAAGTTGCTGCGCTC
  CATGGCCACCCAGGCCATGTCAAACGCACTCTACTTCTCTACGGGATCCTGTGCAGAGGAGGAGTTCCAT
  CATTATGGTCTTGCATTGGATAAATATACCCACTTTACTTCTCCAATAAGAAGATATTCAGATATTGTAG
  TACATCGATTATTAATGGCAGCCATTTCAAAAGACAAGAAAGTGGAAATTAAAGAAAATTTGTTCAGCAA
  CAAAAATCTTGAGGAATTATGCAGACACATCAACAACAGAAACCGGGCGGCACAGCGTTCTCAGAAGCAG
  TCCACCGAGCTCTTCCAGTGCATGTACTTTAAAGACAGAGACCCGGAGACTGAGGAGCGCTGCGTAGTGG
  ATGGAATTATTTATTCCATTAGAACAAATGGTGTACTTGTATTTATACCGAGGTTTGGGATTAAAGGTGC
  TGCTTATCTGAAGAATAAAGATGGCTTAGTGATCTCCTGTGGCCCAGAGGGCAGCTCTGAATGGAAGCCA
  GGATCCCTACAAAGATCTCAAAACAAGATCATCTCTACCACAGCTGGAGGGCAGTCAGTTACATTTCATC
  TGTTTGACCATGTGACGGTAAGGATTTCTGTCCAGCCCTCACGTTGCCACTCTGATATGATCAGGCTTGA
  AATAGTAAGCAACAAACCATACATGATGCCAAACACGGAACTCTGTCATCAGAGCTCCCTCCTGCTGAAG
  AGTGAGCTGGTGAAGGAAGTAACCCGATCTGTGGAGGAAGCGCAGCTTGCACAGGAAGTCAAAGGCAAGG
  TGATTGAGGAAGAGCATCAAGAATACCGCCAGACAAAGGGAAGAAGTCTGTACACGCTTCTGGAGGAGAT
  AAGGGACCTAGCTCTTCTGGATGTTTCCGACAGTTATGCAATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008380
• Insert Size: 2916 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008380.1, NP_001008381.1
• RefSeq Size: 3234 bp
• RefSeq ORF: 2916 bp
• Locus ID: 363077
• UniProt ID: Q5U2P0
• Cytogenetics: 8q24
• Gene Summary: Putative cytoplasm-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA.[UniProtKB/Swiss-Prot Function]