Hcn2 (NM_053684) Rat Untagged Clone

CAT#: RN213404

Hcn2 (untagged ORF) - Rat hyperpolarization activated cyclic nucleotide-gated potassium channel 2 (Hcn2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hcn2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213404 representing NM_053684
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCGCGCGGGGGCGGCGGGCGGCCGGGCGATAGTCCGGGCGCGACCCCTGCGCCGGGGCCGCCGC
  CACCGCCGCCGCCGCCCGCGCCCCCTCAGCCTCAGCCACCACCCGCGCCACCCCCGAACCCCACGACCCC
  CTCGCACCCGGAGTCGGCGGACGAGCCCGGCCCGCGCTCCCGGCTCTGCAGCCGCGACAGCTCCTGCACT
  CCTGGCGCGGCCAAGGGCGGCGCGAATGGCGAGTGCGGGCGCGGGGAGCCGCAGTGCAGCCCCGAGGGCC
  CCGCGCGCGGCCCCAAGGTTTCGTTCTCATGTCGCGGGGCGGCCTCGGGGCCCGCGGCGGCCGAGGAGGC
  GGGCAGCGAGGAGGCGGGCCCGGCGGGTGAGCCGCGCGGCAGCCAGGCCAGCTTCCTGCAGCGCCAATTC
  GGGGCGCTCCTGCAGCCGGGCGTCAACAAGTTCTCCCTGCGGATGTTTGGCAGCCAGAAGGCCGTGGAGC
  GCGAGCAGGAACGCGTGAAGTCGGCGGGGGCCTGGATCATCCACCCCTACAGCGACTTCAGGTTCTACTG
  GGACTTCACCATGCTGTTGTTCATGGTGGGAAATCTCATCATCATCCCTGTGGGCATCACTTTCTTCAAG
  GACGAGACCACGGCGCCCTGGATCGTCTTCAACGTGGTCTCGGACACTTTCTTCCTCATGGACTTGGTGC
  TGAACTTTCGCACCGGCATTGTTATTGAGGACAACACGGAGATCATCCTGGACCCCGAAAAGATAAAGAA
  AAAGTACCTGCGTACGTGGTTCGTGGTAGACTTCGTGTCATCCATCCCGGTGGACTACATCTTCCTCATC
  GTGGAGAAGGGAATCGACTCCGAGGTCTACAAGACGGCCCGTGCACTACGCATCGTGCGTTTCACCAAGA
  TCCTCAGTCTGCTGCGGTTGCTGCGGCTATCCCGGCTCATCCGATATATCCACCAATGGGAGGAGATTTT
  CCACATGACCTACGACCTGGCAAGCGCGGTGATGCGCATCTGTAACCTGATCAGCATGATGCTGCTGCTC
  TGCCACTGGGACGGCTGCCTGCAGTTCCTGGTGCCCATGCTGCAAGACTTCCCCAGCGACTGCTGGGTGT
  CCATCAACAACATGGTGAACCACTCGTGGAGCGAACTCTATTCGTTCGCGCTCTTCAAGGCCATGAGCCA
  CATGCTCTGTATTGGCTACGGGCGGCAGGCTCCCGAGAGCATGACGGACATCTGGCTCACCATGCTCAGC
  ATGATCGTGGGCGCCACCTGCTACGCTATGTTCATTGGGCACGCCACGGCGCTTATCCAGTCCCTGGACT
  CGTCACGGCGCCAGTACCAGGAGAAGTACAAGCAAGTGGAGCAGTACATGTCCTTCCACAAACTGCCGGC
  TGACTTCCGCCAGAAGATCCACGATTACTATGAACACCGGTACCAGGGGAAGATGTTTGACGAGGACAGC
  ATCCTGGGGGAACTCAACGGCCCACTGCGTGAGGAGATTGTGAACTTCAACTGCCGGAAGCTGGTGGCTT
  CCATGCCGTTGTTTGCCAACGCAGACCCCAACTTCGTCACCGCCATGCTGACAAAGCTCAAATTTGAGGT
  CTTCCAGCCTGGAGACTACATCATCCGAGAGGGGACCATCGGGAAGAAGATGTACTTCATCCAGCACGGG
  GTGGTGAGCGTGCTCACCAAGGGCAACAAGGAGATGAAGCTGTCAGATGGCTCCTATTTTGGGGAGATCT
  GCCTGCTCACGAGGGGCCGGCGCACAGCCAGTGTGCGGGCTGACACCTACTGTCGCCTCTACTCACTGAG
  CGTGGACAACTTCAACGAGGTGCTGGAGGAGTACCCCATGATGCGGCGTGCCTTTGAGACCGTGGCCATT
  GACCGCCTGGACCGCATAGGCAAGAAGAACTCCATCTTGCTACACAAGGTTCAGCATGATCTCAGCTCGG
  GTGTGTTCAACAACCAGGAGAACGCCATCATCCAGGAGATTGTCAAATATGACCGTGAGATGGTGCAGCA
  GGCAGAGCTGGGCCAGCGTGTGGGGCTCTTCCCACCACCGCCACCACCGCAGGTCACGTCGGCCATCGCC
  ACACTGCAGCAGGCCGTGGCCATGAGCTTCTGCCCGCAGGTGGCCCGCCCGCTCGTGGGGCCCCTGGCGC
  TAGGGTCCCCACGCCTCGTGCGCCGCGCGCCCCCAGGGCCTCTGCCTCCTGCAGCCTCACCAGGGCCACC
  CGCAGCGAGCCCCCCGGCTGCACCCTCGAGCCCTCGGGCACCGCGGACCTCACCCTACGGTGTGCCTGGC
  TCTCCGGCAACGCGTGTGGGGCCCGCATTGCCCGCACGCCGCCTGAGCCGCGCCTCGCGCCCACTGTCCG
  CCTCGCAGCCCTCGCTGCCCCACGGCGCGCCCGCACCCAGCCCCGCGGCCTCTGCGCGCCCGGCCAGCAG
  CTCCACCCCGCGTCTGGGACCCGCACCCACCACCCGGACCGCGGCACCCAGTCCGGACCGCAGGGACTCA
  GCCTCGCCGGGCGCTGCCAGTGGCCTCGACCCACTGGACTCTGCGCGCTCGCGCCTCTCTTCCAACTTGT
  GA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_053684
• Insert Size: 2592 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053684.1, NP_446136.1
• RefSeq Size: 2633 bp
• RefSeq ORF: 2592 bp
• Locus ID: 114244
• UniProt ID: Q9JKA9
• Cytogenetics: 7q11
• Gene Summary: may play a role in generation of neuronal pacemaker activity [RGD, Feb 2006]