P4hb (NM_012998) Rat Untagged Clone

CAT#: RN213352

P4hb (untagged ORF) - Rat prolyl 4-hydroxylase, beta polypeptide (P4hb), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: P4hb
• Synonyms: PDI; PDIR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213352 representing NM_012998
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGCCGTGCTTTGCTGTGCCTGGCCCTGGCCTGGGCGGCTAGGGTGGGCGCCGACGCTCTGGAGG
  AGGAGGACAACGTCCTGGTGCTGAAGAAGAGCAACTTCGCAGAGGCGCTGGCGGCGCACAACTACCTGCT
  GGTGGAGTTCTATGCCCCATGGTGTGGCCACTGCAAAGCACTGGCCCCAGAGTATGCCAAAGCTGCTGCA
  AAACTGAAGGCAGAAGGCTCTGAGATCCGACTAGCAAAGGTGGACGCCACAGAAGAGTCTGACCTGGCCC
  AGCAGTATGGTGTCCGTGGCTACCCCACAATCAAGTTCTTCAAGAATGGAGACACAGCCTCCCCAAAGGA
  ATATACAGCTGGCAGGGAAGCTGACGACATTGTGAACTGGCTGAAGAAACGCACAGGCCCAGCAGCCACA
  ACCCTGTCTGACACTGCAGCTGCAGAGTCCTTGGTGGACTCAAGCGAAGTGACGGTCATCGGCTTCTTCA
  AGGACGCAGGGTCAGACTCCGCCAAGCAGTTCTTGCTGGCAGCAGAGGCTGTTGATGACATACCTTTTGG
  AATCACTTCCAATAGCGATGTGTTTTCCAAGTACCAGCTGGACAAGGATGGGGTGGTCCTCTTTAAGAAG
  TTTGATGAAGGCCGCAACAATTTTGAAGGTGAGATCACCAAGGAGAAGCTGTTAGACTTCATCAAGCACA
  ACCAGCTGCCTTTGGTCATCGAGTTCACTGAACAGACAGCTCCAAAGATTTTCGGAGGTGAAATCAAAAC
  ACATATTCTGCTGTTCCTGCCCAAGAGTGTGTCTGACTACGATGGCAAATTGAGCAACTTTAAGAAAGCG
  GCCGAGGGCTTTAAGGGCAAGATCCTGTTCATCTTCATCGATAGTGACCACACTGACAACCAGCGCATAC
  TTGAGTTCTTTGGCCTGAAGAAGGAGGAATGTCCAGCTGTGCGGCTTATTACCCTGGAGGAAGAGATGAC
  CAAGTACAAACCGGAGTCAGACGAGCTGACAGCTGAGAAGATCACACAATTTTGCCACCACTTCCTGGAG
  GGCAAGATCAAGCCCCACCTGATGAGCCAGGAACTGCCTGAAGACTGGGACAAGCAGCCAGTGAAAGTGC
  TAGTTGGGAAAAACTTTGAGGAGGTTGCTTTTGATGAGAAAAAGAACGTGTTTGTTGAATTCTATGCTCC
  CTGGTGTGGTCACTGCAAGCAGCTAGCCCCGATTTGGGATAAACTGGGAGAGACATACAAAGACCATGAG
  AATATCGTCATCGCTAAGATGGACTCAACAGCCAATGAGGTGGAAGCTGTGAAGGTGCACAGCTTTCCCA
  CACTCAAGTTCTTCCCAGCAAGTGCAGACAGAACGGTCATTGATTACAACGGTGAGCGGACACTAGATGG
  TTTTAAGAAATTCTTGGAGAGCGGTGGCCAGGATGGAGCGGGGGACAATGACGACCTCGACCTAGAAGAA
  GCTTTAGAGCCAGATATGGAAGAAGACGACGATCAGAAAGCCGTGAAGGATGAACTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012998
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012998.2, NP_037130.2
• RefSeq Size: 2571 bp
• RefSeq ORF: 1530 bp
• Locus ID: 25506
• UniProt ID: P04785
• Cytogenetics: 10q32.3
• Gene Summary: may be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]