Tnfrsf1a (NM_013091) Rat Untagged Clone

CAT#: RN212734

Tnfrsf1a (untagged ORF) - Rat tumor necrosis factor receptor superfamily, member 1a (Tnfrsf1a), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tnfrsf1a
• Synonyms: TNFR-1; Tnfr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212734 representing NM_013091
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCCCCATCGTGCCTGGCCTGCTGCTGTCACTGGTGCTCCTGGCTCTGCTGATGGGGATACACC
  CATCAGGGGTCACCGGACTGGTTCCTTCTCTTGGTGACCGGGAGAAGAGGGATAATTTGTGTCCCCAGGG
  AAAGTATGCCCATCCAAAGAATAATTCCATCTGCTGCACCAAGTGCCACAAAGGAACCTACTTGGTGAGT
  GACTGTCCAAGCCCAGGGCAGGAAACAGTCTGCGAGGTGTGTGATAAAGGCACCTTTACAGCTTCGCAGA
  ACCACGTCAGACAGTGTCTCAGTTGCAAGACATGTCGGAAAGAAATGTTCCAGGTGGAGATTTCTCCTTG
  CAAAGCTGACATGGACACCGTGTGTGGCTGCAAGAAGAACCAATTCCAGCGCTACCTGAGTGAGACGCAT
  TTCCAGTGTGTGGACTGCAGCCCCTGCTTCAATGGCACCGTGACAATCCCCTGTAAGGAGAAACAGAACA
  CCGTGTGTAACTGCCACGCAGGATTCTTTCTAAGCGGAAATGAGTGCACCCCTTGCAGCCACTGCAAGAA
  AAATCAGGAATGTATGAAGCTGTGCCTACCTCCAGTTGCAAATGTCACAAACCCCCAGGACTCAGGTACT
  GCCGTGCTGTTGCCTCTGGTTATCTTCCTAGGTCTTTGCCTTTTATTCTTTATCTGCATCAGTCTACTGT
  GCCGATATCCCCAGTGGAGGCCCAGGGTCTACTCCATCATTTGTAGGGATTCAGCTCCTGTCAAAGAGGT
  GGAGGGTGAAGGAATTGTTACTAAGCCCCTAACTCCAGCCTCTATCCCAGCCTTCAGCCCCAACCCCGGC
  TTCAACCCCACTCTGGGCTTCAGCACCACCCCACGCTTCAGTCATCCTGTCTCCAGTACCCCCATCAGCC
  CCGTCTTCGGTCCTAGTAACTGGCACAACTTCGTGCCACCTGTAAGAGAGGTGGTCCCAACCCAGGGTGC
  TGACCCTCTCCTCTACGGATCCCTCAACCCTGTGCCAATCCCCGCCCCTGTTCGGAAATGGGAAGACGTC
  GTCGCGGCCCAGCCACAACGGCTTGACACTGCAGACCCTGCGATGCTGTATGCTGTGGTGGATGGCGTGC
  CTCCGACACGCTGGAAGGAGTTCATGCGGCTCCTGGGGCTGAGCGAGCACGAGATCGAGCGGCTGGAGCT
  GCAGAACGGGCGTTGCCTCCGCGAGGCTCATTACAGCATGCTGGAAGCCTGGCGGCGCCGCACACCGCGA
  CACGAGGCCACGCTGGACGTAGTGGGCCGCGTGCTTTGCGACATGAACCTGCGTGGCTGCCTGGAGAACA
  TCCGCGAGACTCTAGAAAGCCCTGCCCACTCGTCCACGACCCACCTCCCGCGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013091
• Insert Size: 1386 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013091.1, NP_037223.1
• RefSeq Size: 2130 bp
• RefSeq ORF: 1386 bp
• Locus ID: 25625
• UniProt ID: P22934
• Cytogenetics: 4q42
• Gene Summary: may play a role in TNFalpha mediated inflammatory response to spinal cord injury [RGD, Feb 2006]