Arpp19 (NM_031660) Rat Untagged Clone

CAT#: RN212697

Arpp19 (untagged ORF) - Rat cyclic AMP phosphoprotein (Arpp-19), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Arpp19
• Synonyms: Arpp-19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212697 representing NM_031660
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCGGAAGTCCCCGAGGCAGCGTCGGCGGAGGAGCAAAAAGAAATGGAAGATAAAGTAACTAGTC
  CAGAGAAAGCTGAAGAAGCAAAGTTAAAAGCAAGGTATCCTCATTTGGGACAAAAGCCTGGTGGTTCTGA
  CTTTTTAAGGAAAAGATTGCAGAAAGGGCAAAAGTATTTTGATTCTGGGGATTACAACATGGCAAAAGCA
  AAGATGAAGAACAAGCAGCTTCCTGCTGCAGCCCCGGATAAGACAGAGGTCACTGGTGACCACATTCCCA
  CTCCACAGGACCTTCCTCAGCGGAAACCATCCCTTGTTGCTAGCAAACTGGCTGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031660
• Insert Size: 339 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031660.1, NP_113848.1
• RefSeq Size: 339 bp
• RefSeq ORF: 339 bp
• Locus ID: 60336
• UniProt ID: Q712U5
• Cytogenetics: 8q24
• Gene Summary: Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-62 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). May indirectly enhance GAP-43 expression by binding to the NGF-regulatory region of its mRNA.[UniProtKB/Swiss-Prot Function]