Cnr1 (NM_012784) Rat Untagged Clone

CAT#: RN212368

Cnr1 (untagged ORF) - Rat cannabinoid receptor 1 (brain) (Cnr1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cnr1
• Synonyms: SKR6R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212368 representing NM_012784
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCGATCCTAGATGGCCTTGCAGACACCACCTTCCGTACCATCACCACAGACCTCCTCTACGTGG
  GCTCGAATGACATTCAGTATGAAGATATCAAAGGAGACATGGCATCCAAATTAGGATACTTCCCACAGAA
  ATTCCCTCTAACTTCCTTCAGGGGTAGTCCCTTCCAAGAAAAGATGACCGCAGGAGACAACTCCCCGTTG
  GTCCCAGCAGGAGACACAACAAACATTACAGAGTTCTATAACAAGTCTCTCTCGTCGTTCAAGGAGAATG
  AGGAGAACATCCAGTGTGGGGAGAACTTTATGGACATGGAGTGCTTTATGATTCTGAATCCCAGCCAGCA
  GCTGGCCATCGCTGTACTGTCCCTCACACTGGGCACCTTCACGGTTCTGGAGAACCTACTGGTGCTGTGT
  GTCATCCTGCACTCCCGCAGTCTCCGATGCAGGCCTTCCTACCACTTCATCGGCAGCCTGGCAGTGGCCG
  ACCTCCTGGGAAGTGTCATTTTTGTGTACAGCTTTGTTGACTTCCATGTATTCCACCGTAAAGACAGCCC
  CAATGTGTTTCTGTTCAAACTGGGTGGGGTTACAGCCTCCTTCACAGCTTCTGTGGGCAGCCTGTTCCTC
  ACAGCCATCGACAGGTACATATCCATTCACAGGCCTCTGGCCTATAAGAGGATCGTCACCAGGCCCAAGG
  CCGTTGTGGCCTTTTGCCTGATGTGGACTATCGCAATAGTAATCGCTGTGTTGCCTCTCCTGGGCTGGAA
  CTGCAAGAAGCTGCAATCTGTTTGCTCGGACATTTTCCCACTCATTGACGAGACCTACCTGATGTTCTGG
  ATTGGGGTGACCAGTGTGCTGCTGCTGTTCATTGTGTACGCGTACATGTACATTCTCTGGAAGGCTCACA
  GCCACGCGGTCCGCATGATTCAGCGTGGGACCCAGAAGAGCATCATCATCCACACGTCAGAAGACGGCAA
  GGTGCAGGTGACCCGGCCTGACCAAGCCCGCATGGACATTAGGCTGGCCAAAACCCTGGTTCTGATCCTG
  GTGGTGTTGATCATCTGCTGGGGCCCTCTGCTTGCGATCATGGTGTATGACGTCTTCGGGAAGATGAACA
  AGCTTATCAAGACGGTGTTTGCCTTCTGCAGTATGCTCTGCCTGCTGAACTCCACCGTGAACCCCATCAT
  CTATGCTCTGAGGAGCAAGGACCTGAGACATGCTTTCCGAAGCATGTTCCCTTCGTGCGAAGGCACCGCA
  CAGCCTCTAGACAACAGCATGGGGGACTCAGACTGCCTGCACAAGCACGCCAACAACACAGCCAGCATGC
  ACAGGGCCGCGGAGAGCTGCATCAAGAGCACCGTTAAGATCGCGAAGGTGACCATGTCTGTGTCCACAGA
  CACGTCCGCCGAGGCTCTGTGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_012784
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012784.4, NP_036916.1
• RefSeq Size: 5465 bp
• RefSeq ORF: 1422 bp
• Locus ID: 25248
• UniProt ID: P20272
• Cytogenetics: 5q21
• Gene Summary: G-protein coupled receptor for psychoactive cannabinoids; may mediate inhibition of NO production and participate in retrograde signaling by endocannabinoids [RGD, Feb 2006]