Zyx (NM_053761) Rat Untagged Clone

CAT#: RN212301

Zyx (untagged ORF) - Rat zyxin (Zyx), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Zyx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212301 representing NM_053761
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCCCGCCCGCCTCCCGCGATCTCCGTCTCGGTCTCGGCCCCCGCGTTTTACGCCCCGCAGA
  AGAAGTTCGCCCCGGTTGTGGCCCCGAAGCCCAAAGTGAATCCTTTCCGGCCTGGGGACAGCGAGTCTCC
  TGTGGCAGCCGGGGCCCAGAGAGCACAGATGGGCCGGGTGGGCGAGATCCCCCCACCGCCCCCGGAAGAC
  TTTCCTTTGCCCCCTCCTCCCGTTATTGGGGAGGGCGACGACTCAGAGGGGGCCCTGGGAGGTGCCTTCC
  CACCTCCACCTCCCCCGTTGATCGAAGAACCATTCCCCCCTGCTCCTCTGGAGGAGGACATCTTCCCCTC
  CCCTCCACCTCCACTGGAGGAGGAGGGAGGACCTGAGGCCCCTACCCAGCTCCCACCGCAGCCCAGGGAG
  AAAGTGAGCAGTATTGACCTGGAGATCGACTCTCTGTCCTCACTGCTGGATGACATGACCAAGAACGATC
  CCTTCAAAGCCCGGGTGTCATCTGGATATGTACCTCCACCAGTTGCTACTCCATTTGTTCCCAAACCTAG
  TACCAAGCCTGCCCCTGTGGGCACAGCACCCTTACCTCCTTGGAAGGCCCCTTCTAGCTCTCAGCCACCA
  CCTCAGCCGCAGGCCAAGCCTCAGGTCCAGCTCCATGCCCAGCCTCAGGCCAAGCCCCATGTCCAACCCC
  AGTCTGTGTCTTCCGCTAATACACAGCCCCGAGGTCCCCTTTCTCAGGCTCCAACTCCAGCACCTAAGTT
  TGCTCCAGTGGCACCTAAATTTACTCCCGTGGCTTCCAAATTCAGCCCTGGTGCTCCAAGTGGACCTGGG
  CCACAGCCCAGTCAAAAATCGGTGCCTCTGGAAGCTCCTTCTTCTGTGGGCACAGGCTCCCCCCAGCCCC
  CTAGCTTCACCTATGCTCAGCAGAAGGAGAAGCCCCTAGTTCAAGAGAAGCAGCACCCACTGCCTCCACC
  AGCTCAAAACCAAAACCAGGTACGTTCTCCTGGAGGCCCAGGCCCCTTGACCCTGAAGGAGGTGGAAGAG
  TTGGAGCAGCTGACCCAGCAGCTGATGCAGGACATGGAGCACCCTCAGAGGCAGAGCGTGGCAGTAAATG
  AGTCCTGTGGCAAATGCAGTCAGCCACTGGCCCGAGCACAGCCTGCAGTCCGTGCACTGGGACAACTGTT
  CCATATCACCTGCTTCACTTGTCATCAGTGTCAGCAGCAGCTGCAGGGACAGCAGTTCTATAGCCTGGAG
  GGGGCACCATATTGTGAGGGCTGCTACACTGACACTCTGGAGAAGTGCAACACTTGTGGGCAGCCCATCA
  CTGACCGAATGCTGAGGGCCACTGGCAAAGCCTACCACCCACATTGCTTCACCTGTGTGGTCTGCGCCTG
  TCCCCTGGAGGGCACTTCCTTCATTGTGGACCAGGCCAATCAGCCCCACTGTGTTCCTGACTATCACAAG
  CAATATGCTCCAAGGTGCTCTGTCTGCTCGGAGCCCATCATGCCTGAGCCTGGCCGGGATGAGACTGTGC
  GAGTAGTCGCGCTGGATAAGAACTTTCATATGAAGTGTTACAAGTGTGAGGACTGTGGGAAAGCCCTGTC
  CATTGAGGCAGATGACAACGGCTGTTTCCCTCTGGATGGCCATGTCCTTTGTCGGAAGTGCCACTCTGCT
  AGAGCCCAGACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053761
• Insert Size: 1695 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053761.1, NP_446213.1
• RefSeq Size: 2558 bp
• RefSeq ORF: 1695 bp
• Locus ID: 114636
• Cytogenetics: 4q24
• Gene Summary: may be involved in regulation of inflammatory response; displays decreased mRNA expression in vascular smooth muscle cells treated with cytokines IL-1 and TNF alpha [RGD, Feb 2006]