Pole (NM_001107152) Rat Untagged Clone

CAT#: RN212034

Pole (untagged ORF) - Rat polymerase (DNA directed), epsilon (Pole), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pole
• Synonyms: POLE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212034 representing NM_001107152
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGAGGAACAGTGGACGGAGGCATCCCGAGCCGGGCGCGGACAGCGAAGGCAGCCGGGATGATG
  GTCCCTCCTCTTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACGGATAAGATGGACTTACG
  GTTTGGTTTTGAAAGGTTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTTATCAACATGCACCCTACAGAG
  ATTTTAGATGAAGACAAACGCTTAGTCAGTGCAGTGGATTACTACTTCATTCAAGATGATGGAAGCAGAT
  TTAAGGTGGCCTTGCCCTACAAGCCATATTTCTACATTGCAGCTAGAAAGGGTTGTGATCGAGAAGTTTC
  ATCTTTTCTATCCAAGAAGTTTCAGGGTAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGACCTGGAC
  TTGCCAAACCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGATCTTG
  TCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGACGAGTA
  CACAACAATGCTCTCCAGTATTCTGCAAGGAGGCAGTCTAATTACTGATGAGGAGGAAACCTCTAAGAAG
  ATAGCTGACCAATTGGACAACATAGTGGACATGCGGGAGTATGATGTTCCCTACCACATTCGCCTCTCCA
  TTGACCTCAGAATCCATGTGGCCCACTGGTACAATGTTAGATTTCGAGGAAATGCTTTTCCTGTGGAAAT
  TGCCCGACGAGATGATCTCGTCGAACGACCTGATCCTGTGGTTTTGGCATTTGACATTGAGACAACCAAA
  CTACCTCTCAAATTCCCTGATGCTGAGACAGATCAGATTATGATGATCTCCTATATGATTGATGGCCAGG
  GCTACCTCATCACTAACAGGGAGATTGTTTCAGAAGACATTGAAGATTTTGAGTTCACCCCCAAGCCAGA
  ATATGAAGGGCCTTTCTGTGTCTTCAATGAACCTGATGAGGTCCATCTGATCCAGAGATGGTTTGAGCAT
  ATCCAGGAGACCAAACCTACCATTATGGTCACCTACAATGGGGATTTTTTTGACTGGCCGTTTGTGGAGG
  CCAGGGCAGCAATTCATGGCCTAAGCATGTACCAGGAGATAGGCTTCCAGAAGGATAGCCAGGGGGAATA
  TAAGGCACCACAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGTGGGC
  AGTCATAATCTCAAGGCAGCTGCCAAAGCCAAACTTGGCTATGACCCTGTGGAGCTGGACCCTGAGGACA
  TGTGCCGTATGGCCACTGAACAGCCCCAGACTCTGGCCACTTACTCAGTGTCAGATGCTGTTGCTACTTA
  TTACCTGTACATGAAATATGTGCACCCCTTCATATTCGCCCTCTGCACCATTATTCCCATGGAACCTGAT
  GAGGTGCTGCGGAAGGGCTCTGGAACATTGTGCGAAGCCTTGCTGATGGTGCAAGCCTTCCATGCCAACA
  TTATCTTTCCCAATAAGCAAGAGCAGGAGTTCAACAAGCTGACAGATGATGGCCACGTGCTAGATGCTGA
  GACCTACGTTGGGGGCCATGTGGAGGCCCTAGAGTCTGGTGTCTTCCGAAGTGATATCCCCTGCCGGTTT
  AGGATGAATCCTGCAGCCTTTGATTTCCTGCTGCAGCGAGTTGAGAAGACTATGCGCCATGCCATTGAAG
  AAGAAGAGAAGGTGCCTGTGGAACAAGCCACCAACTTCCAAGAGGTGTGTGAGCAGATTAAGACCAAACT
  CACCTCCCTAAAAGATGTTCCTAACAGAATTGAATGTCCTCTAATCTACCATCTAGATGTGGGGGCCATG
  TATCCTAACATAATCCTCACCAACCGCCTGCAGCCTTCTGCCATAGTGGATGAGGCCACCTGTGCTGCCT
  GTGACTTCAATAAGCCTGGAGCAACTTGTCAGAGGAAAATGGCCTGGCAATGGAGGGGAGAATTCATGCC
  AGCCAGTCGTAGTGAGTACCATCGAATCCAGCATCAGCTAGAGTCGGAGAAGTTCCCCCCTTTGTTCCCA
  GAGGGGCCAGCAAGGGCCTTTCATGAGCTGTCCCGTGAAGAACAGGCTAAATACGAGAAACGGAGGCTGG
  CAGATTATTGCCGGAAAGCCTATAAGAAGATCCATATGACCAAGGTAGAAGAGCGCCTGACTACCATCTG
  CCAGCGGGAAAACTCATTTTATGTGGACACAGTGCGGGCCTTCAGAGACAGGCGCTATGAATTCAAAGGA
  CTGCACAAGGTGTGGAAGAAGAAGCTCTCGGCGGCTGTAGAGGTGGGCGATGCATCAGAGGTGAAGCGCT
  GCAAGAACATGGAGATCCTTTATGATTCGCTGCAGCTGGCTCACAAATGCATCCTGAACTCATTCTACGG
  CTATGTTATGCGCAAAGGAGCTCGCTGGTACTCCATGGAGATGGCTGGTATCGTCTGCTTTACAGGAGCC
  AACATCATCACCCAGGCAAGAGAGCTGATTGAGCAGATCGGGAGGCCTTTAGAACTGGACACGGACGGAA
  TATGGTGCGTTCTACCCAATAGCTTTCCTGAAAATTTTGTCATCAAGACAACCAATGTGAAAAAACCCAA
  ACTGACCATCTCCTATCCTGGTGCCATGTTGAACATCATGGTCAAGGAAGGCTTTACCAACCACCAGTAC
  CAGGAACTAACAGAGCCTTCGTCTCTCACCTATGTCACCCGCTCTGAGAACAGCATCTTTTTTGAAGTTG
  ATGGACCATACCTTGCTATGATCCTTCCAGCCTCCAAGGAAGAAGGCAAGAAGCTGAAGAAAAGATATGC
  TGTATTCAATGAAGATGGCTCCTTGGCTGAACTGAAAGGTTTTGAGGTGAAACGCCGAGGGGAGTTGCAG
  TTGATTAAAATCTTCCAGTCCTCAGTGTTTGAGGCCTTCCTCAAGGGCAGCACACTAGAGGAGGTGTATG
  CCTCTGTGGCCAAAGTGGCTGACTACTGGCTAGATGTGCTCTATAGCAAGGCTGCTAATATGCCCGATTC
  TGAATTATTCGAACTGATTTCTGAGAACCGCTCCATGTCACGGAAGCTAGAAGATTATGGGGAGCAGAAG
  TCTACGTCCATCAGCACAGCAAAGCGCCTGGCTGAGTTCCTGGGAGACCAGATGGTCAAAGATGCTGGAC
  TGAGCTGCCGCTACATTATCTCCCGAAAGCCAGAGGGATCTCCTGTCACTGAGAGGGCCATTCCACTTGC
  CATTTTCCAAGCAGAGCCTACAGTGAGGAAACATTTTCTCCGGAAATGGTTAAAGAGTTCATCACTTCAA
  GACTTTGATATTCGGACAATTCTGGACTGGGACTACTACATAGAGCGGCTGGGGAGTGCCATCCAGAAAA
  TCATCACCATCCCTGCAGCTCTACAGCAGGTGAAGAACCCAGTTCCACGTGTCAAACATCCAGACTGGCT
  GCACAAAAAGCTACTAGAGAAGAATGATATCTACAAACAGAAGAAGATCAGTGAGCTCTTTGTGCTTGAA
  GGAAAGAGACAGATTGTGATGGCCCAGGCTTCAGAAAACAGTCTGAGTCTCTGCACTCCAGACATGGAGG
  ACTTTGGACTCACAAAGCCATATCACTCTACAGTTCCAGTTGCTACTAAGAGGAAGCGAGTCTGGGAGAG
  CCAAAAGGAGTCTCAGGATATTACACTAACTGTGCCCTGGCAAGAGGTCTTAGGGCAGCCTCCCTCCCTT
  GGAACCACACAGGAAGAGTGGTTGGTCTGGCTCCAGTTCCACAAGAAAAAGTGGCAGCTGCAGGCTCAAC
  AGCGCCTAGCCCGCAGGAAGAAACAGCGCTTAGAGTCAGCAGAAGATATGCCAAGGCTTGGGGCTATCCG
  AGAGGGGCCTGCCACAGGACTGGGGAGCTTTCTGCGACGGACCGCCCGCAGCATCATGGACCTCCCCTGG
  CAGATCATACAGATCAGTGAGACCAGACAGGCTGGTCTGTTCCGGCTGTGGGCTATCATTGGCAGCGACT
  TGCACTGCATCAAGCTGAGTATCCCTCGAGTATTCTATGTAAACCAGCGGGTAGCCAAAGCAGAGGATGG
  GCCTTCGTATCGAAAGGTGAATCGGGCTCTTCCTCGTTCCAACATCGTCTACAATCTCTATGAGTACTCA
  GTACCAGAGGACATGTACCAAGAACACATCAACGAGATCAACACTGAGTTGTCAGCACCAGACATTGAGG
  GCGTGTATGAGACTCAGGTCCCATTGTTATTCCGGGCCCTGGTGCAGCTGGGCTGTGTGTGTGTGGTCAA
  CAAACAGCTGGCAAGGCAACTTTCAGGCTGGGAAGCTGAAACCTTTGCCCTCGAGCACCTTGAAATGCGT
  TCTCTGGCCCAGTTCAGCTACTTGGAACCAGGGAGTATCCGCCACATCTACCTGTACCATCACACACAGG
  GCCACAAGGCACTCTTTGGGGTCTTTATCCCCTCACAGCGAAGAGCATCTGTGTTTGTGTTGGATACTGT
  GCGCAGCAACCAAATGCCAGGGCTCAGTGCCCTATACTCAGCAGAACACAGCCTGCTGCTGGACAAGGTG
  GGCCCCAACCTCCTGCCTCCCCCAAAACACACCTTTGAAGTTCGTGCTGAAACCAACCTGAAGACCATCT
  GCAGAGCCATCCAACGCTTCCTGCTTGCCTACAAGGAAGAGCGCCGGGGGCCCACGCTCATCGCTATCCA
  GTCTAGCTGGGAACTGTGTAGGCTGACCAGTGAGATTCCAGTCTTAGAGGAGTTCCCACTAGTGCCTGTC
  CGAGTGGCTGACAAGATCAGCTATGCAGTCCTGGACTGGCAGCGCCACGGAGCTCGCCGAATGATCCGGC
  ACTACCTCAATTTAGACATTTGCCTTTCACAGGCCTTTGAGATGAGCAGGTACTTCCACATCCCCGTTGG
  AAACCTGCCAGAAGACATCTCCACCTTTGGCTCTGACCTCTTTTTTGCACGCCATCTCCAGCGCCATAAC
  CACCTACTTTGGTTGTCCCCTACCTCTCGGCCTGACCTGGGTGGGAAGGAAGCTGATGACAACCGCCTTG
  TCATGGAGTTTGATGACCGAGCCACTGTGGAGATCAATAATTCCGGCTGTTACTCTACTGTGTGCGTGGA
  GCTGGACATTCAAAACCTGGCAGTCAACACCATCCTCCAGTCCCACCATGTCAATGACATGGAGGGGGCT
  GGCAGCATGGGCATCAGCTTCGATGTGATCCAGCAGGCCTCCCTAGAGGACATGGTTACAGGCAATCAAG
  CTGCCAGTGCCCTGGCCAGCTACGATGAGACAGCCCTCTGCTCCAGTACGTTCAGGATCCTGAAGAGCAT
  GGTGGTTGGCTGGGTAAAGGAAATCACACAGTACCACAACATCTATGCTGACAACCAGGTGATGCACTTC
  TACCGCTGGCTCCAGTCGCCGTGCTCTCTGCTCCACGACCCAGCCCTTCACCGGACACTGCACAATATGA
  TGAAGAAGCTCTTCCTGCAGCTCATTGCTGAGTTCAAGCGCCTGGGGTCATCGGTCATCTATGCCAACTT
  CAATCGCATCATCCTCTGTACAAAGAAGCGCCGGATAGAGGATGCCCTTGCCTATGTGGAATATATCACC
  AACAGCATCCATTCTAAAGAGATCTTTCATTCCCTGACCATCTCTTTCTCTCGATGCTGGGAATTCCTTC
  TCTGGATGGATCCATCCAACTATGGTGGAATCAAAGGAAAAGTCCCATCCAGTATTCACTGTGGACAAGT
  AAGGGAGCAAGACTCCCAGACAAGGGAGGAAACTGAGGATGAGGAGGACAATGAAAAGGATGAGGAGGAA
  GAGGAGGACATGGGGCAATCTGAGGTTGAGGACTTACTGGAGAACAACTGGAACATTCTACAGTTCTTGC
  CACAGGCAGCCTCTTGCCAGAGCTACTTCCTTATGATCGTTTCAGCATACATCGTGGCTGTGTACCAAAG
  CATGAAGGATGAGTTGAGACACAGTGCCCCAGGCAGTACCCCTGTGAAGAGGAAGGGGGCCAGCCAGTTC
  TCCCAGGAGGCTGAAGGGGCAGCCGGGGCCCTTCCTGGAATGATCACTTTCTCTCAAGATTATGTGGCAA
  ATGAGCTCACTCAGACCTTCTTCACCATTACTCAGAAAATTCAGAAGAAAGTCACAGGCTCTCGGAACAC
  CGCTGAGCCCTCAGAGATGTTCCCTGTCCTCCCTGGTTCACATTTGCTGCTCAATAACCCTGCTCTGGAG
  TTCATCAAATACGTGTGCAAGGTACTATCTCTGGATACAAACATCACAAATCAGGTGAATAAGCTAAACA
  GAGACCTGCTTCGCCTGGTAGACGTTGGTGAATTCTCTGAGGAGGCCCAGTTCAGAGACCCCTGCCACTC
  CTACGTGCTCCCTGAGGTAATCTGCCACAGCTGTAATTTCTGCCGAGACCTGGACCTATGCAAAGATTCT
  TCTTTCTCTCAGGACGGAGCCATCCTGCCTCAGTGGCTTTGCTCCAATTGTCAGGCCCCCTACGACTCAT
  CTGCCATTGAGTCAGCCTTGGTGGAAGCCCTGCAGAGGAAACTGATGGCCTTCACACTTCAGGACCTGGT
  GTGCCTCAAGTGCCGTGGTATGAAAGAGACCCATATGCCTGTGTACTGCGGCTGCGCAGGGGACTTTGTT
  CTCACCATCCATACTGAGGTCTTCATGGAACAGATTAGAATCTTCCAGAACATTGCCAAGTACTACAACA
  TGTCATATCTCCAAGAGGCCATAGAATGGCTGCTACAGACAAGCCCTGTATCAAACTTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001107152
• Insert Size: 6852 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107152.2, NP_001100622.2
• RefSeq Size: 7101 bp
• RefSeq ORF: 6852 bp
• Locus ID: 304573
• Cytogenetics: 12q16