Ube2s (NM_001106224) Rat Untagged Clone

CAT#: RN211808

Ube2s (untagged ORF) - Rat ubiquitin-conjugating enzyme E2S (Ube2s), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ube2s
• Synonyms: RGD1564746
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211808 representing NM_001106224
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCAATGTAGAGAATCTCCCTCCCCACATCATCCGCCTAGTGTACAAGGAGGTGACAACACTGA
  CAGCAGATCCACCCGATGGCATTAAAGTCTTCCCCAATGAGGAGGATCTCACCGACCTGCAGGTTACAAT
  CGAGGGCCCTGAAGGGACCCCCTATGCTGGAGGTCTGTTCCGTATGAAGCTCCTACTAGGGAAGGACTTC
  CCTGCCTCCCCACCCAAGGGCTACTTCCTGACTAAAATCTTCCACCCAAATGTGGGCCCCAATGGTGAGA
  TCTGTGTCAACGTGCTCAAGAGGGACTGGACTGCTGAGTTGGGCATACGACATGTGCTGCTGACCATCAA
  GTGCCTGCTGATCCATCCTAACCCAGAGTCTGCACTCAATGAGGAGGCAGGCCGCCTGCTTTTGGAGAAC
  TACGAGGAGTATGCTGCCCGGGCCCGTCTGCTCACAGAGATCCATGGGGGTGCATGTAGCACCAGCAGTG
  GGAGGGCTGAGGCTTCCCGGGACCTGGCCAGTGGGGCTTCAGCCTCCTCCACTGACCCTATGACCCCAGG
  GGTCCTAGGAGGAGCTGAGGGTCCCATGGCCAAGAAACATGCAGGTGAGCGAGATAAGAAGCTGGCAGCC
  AAGAAAAAGTTGGACAAGAAGCGAGCACTGAGGCGACTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001106224
• Insert Size: 672 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106224.2, NP_001099694.1
• RefSeq Size: 991 bp
• RefSeq ORF: 672 bp
• Locus ID: 292588
• UniProt ID: B5DFI8
• Cytogenetics: 1q12
• Gene Summary: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function]