Wnt10a (NM_001108227) Rat Untagged Clone

CAT#: RN211332

Wnt10a (untagged ORF) - Rat wingless-type MMTV integration site family, member 10A (Wnt10a), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Wnt10a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211332 representing NM_001108227
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCGCCCACCCTCGCCCCTGGCTGCGGCTCCCACCAGGGCCCCAGCCGCGGCCTGAGCTCTGGG
  CGCTCCTGTTCTTCCTACTGCTGCTGGCTGCTGCTGTGCCCAGGTCAGCACCCAACGACATCCTGGGCCT
  CCGCCTACCCCCAGAGCCTGTGCTCAACGCCAACACAGTGTGCCTGACATTGCCCGGCCTGAGCCGGCGG
  CAGATGGAGGTGTGTGTGCGTCACCCTGACGTGGCCGCCTCTGCTATCCAGGGCATCCAGATCGCCATCC
  ATGAGTGCCAGCATCAGTTCCGGGACCAGCGCTGGAACTGTTCCAGCCTGGAGACTCGGAACAAAGTCCC
  CTACGAGAGCCCCATCTTCAGCAGAGGTTTTCGAGAGAGTGCTTTCGCCTACGCCATAGCAGCTGCCGGG
  GTGGTGCACGCAGTGTCCAACGCGTGCGCCCTGGGTAAACTGAAGGCTTGCGGTTGCGATGCCTCCAGGC
  GTGGGGACGAAGAGGCCTTTCGTCGGAAGCTGCACCGCTTGCAGCTGGACGCCCTACAGCGCGGAAAGGG
  CTTGAGCCACGGGGTCCCGGAGCACCCTGCCATACCCCCTGCCAGCCCAGGCCTGCAGGACTCCTGGGAG
  TGGGGTGGCTGCAGTCCGGATGTGGGCTTTGGAGAACGCTTCTCCAAGGACTTTCTGGACTCCCGAGAGC
  CTCACAGAGACATCCACGCACGCATGAGACTCCACAACAACCGTGTGGGCAGGCAGGCGGTGATGGAGAA
  CATGCGGCGAAAGTGCAAGTGCCACGGCACCTCAGGCAGCTGCCAGCTCAAGACCTGCTGGCAGGTGACG
  CCGGAGTTCCGCACAGTGGGGGCGCTGCTGCGCAACCGCTTCCACCGCGCCACGCTCATCCGGCCGCACA
  ACCGCAACGGTGGCCAGTTGGAGCCCGGCCTCGCGGGAGCACCCTCGCCAGCACCGGGTACTCCAGGGCT
  GCGCCGTAGGGCCAGTCACTCCGACCTGGTCTACTTTGAGAAATCGCCCGACTTCTGTGAGCGCGAGCCT
  CGTCTGGACTCGGCAGGCACTGTGGGGCGCCTGTGCAATAAGAGCAGCACGGGTCCAGACAGCTGCGGCA
  GCATGTGCTGTGGCCGCGGTCACAACATTCTGCGCCAGACGCGCAGCGAGCGCTGCCACTGTCGTTTCCA
  CTGGTGCTGCTTCGTGGTCTGCGAAGAATGCCGCATCACTGAGTGGGTCAGCGTCTGCAAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001108227
• Insert Size: 1254 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108227.1, NP_001101697.1
• RefSeq Size: 2466 bp
• RefSeq ORF: 1254 bp
• Locus ID: 316527
• Cytogenetics: 9q33