Gabra4 (NM_080587) Rat Untagged Clone

CAT#: RN211099

Gabra4 (untagged ORF) - Rat gamma-aminobutyric acid (GABA) A receptor, alpha 4 (Gabra4), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gabra4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211099 representing NM_080587
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCTGTCCAGAAGGTACCTGCGATCGTGCTGTGCTCCGGGGTCAGCCTCGCCCTCCTGCACGTCC
  TGTGCCTGGCGACTTGTTTAAACGAATCCCCAGGACAGAATTCAAAGGACGAGAAATTGTGCCCGGAAAA
  TTTTACCCGTATCCTGGACAGTTTGCTGGATGGTTATGACAACAGACTGCGTCCTGGATTTGGGGGTCCT
  GTTACAGAGGTGAAAACTGATATATATGTCACCAGCTTTGGACCCGTTTCTGATGTTGAAATGGAATACA
  CAATGGATGTGTTCTTCAGACAGACATGGATTGACAAAAGACTGAAATATGATGGCCCCATTGAAATCCT
  GAGGTTGAACAATATGATGGTCACCAAAGTTTGGACCCCTGATACTTTCTTCAGGAATGGAAAGAAATCT
  GTCTCCCATAACATGACAGCTCCAAATAAACTTTTTAGAATTATGAGAAATGGCACTATTTTATACACAA
  TGAGACTCACCATAAGTGCGGAGTGTCCCATGAGACTGGTGGATTTTCCTATGGACGGTCATGCCTGCCC
  TTTGAAATTTGGGAGTTATGCATATCCCAAGAGTGAGATGATCTACACCTGGACCAAAGGCCCTGAGAAG
  TCAGTGGAGGTACCAAAGGAGTCCTCGAGCTTAGTTCAGTATGATCTAATTGGCCAGACTGTATCCAGTG
  AGACTATCAAATCTATTACAGGTGAATACATTGTTATGACCGTGTACTTTCACCTCAGACGGAAGATGGG
  CTATTTTATGATTCAGACATATATCCCGTGCATCATGACAGTGATCCTTTCTCAAGTTTCCTTCTGGATC
  AATAAGGAGTCTGTTCCAGCCAGAACTGTATTTGGAATAACCACAGTCCTCACGATGACCACCCTAAGCA
  TCAGTGCTCGGCATTCTTTGCCCAAAGTGTCCTATGCGACTGCCATGGATTGGTTCATAGCTGTCTGTTT
  TGCTTTTGTATTTTCGGCTCTTATTGAGTTTGCTGCTGTCAACTATTTCACCAACATTCAAATGCAAAAA
  GCCAAAAAGAAGATATCAAAACCTCCTCCAGAAGTTCCAGCTGCCCCAGTACTGAAGGAAAAACATACAG
  AAACATCTCTTCAGAATACACATGCTAATTTGAACATGAGGAAAAGAACAAATGCATTAGTCCACTCAGA
  ATCAGATGTCAACAGCAGAACTGAGGTGGGGAACCATTCCAGCAAGACCACCGCTGCCCAGGAGTCTTCT
  GAAACCACTCCTAAGGCCCACTTGGCTTCCAGTCCAAATCCATTCAGCAGGGCAAATGCAGCTGAGACTA
  TCTCTGCAGCAGCAAGAGGTCTTTCGTCTGCCGCATCGCCCTCTCCTCACGGGACACTGCAGCCAGCTCC
  TTTGCGGTCGGCGTCTGCTCGCCCGGCATTTGGAGCTAGACTTGGGCGCATTAAGACAACAGTTAATACG
  ACAGGGGTGCCTGGGAATGTGTCAGCCACACCTCCTCCCTCTGCTCCACCACCTTCTGGATCTGGCACAA
  GTAAAATAGACAAATATGCTCGTATTCTCTTTCCAGTCACATTTGGGGCATTTAACATGGTCTACTGGGT
  GGTTTATTTATCTAAGGACACCATGGAGAAATCAGAAAGTCTAATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_080587
• Insert Size: 1659 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080587.3, NP_542154.3
• RefSeq Size: 1843 bp
• RefSeq ORF: 1659 bp
• Locus ID: 140675
• UniProt ID: P28471
• Cytogenetics: 14p11
• Gene Summary: alpha 4 subunit of the GABA-A receptor which binds gamma-aminobutyric acid; involved in mood and anxiety [RGD, Feb 2006]