Cry2 (NM_133405) Rat Untagged Clone

CAT#: RN210144

Cry2 (untagged ORF) - Rat cryptochrome 2 (photolyase-like) (Cry2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cry2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210144 representing NM_133405
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCTGCTGTGGTGGCAGCGACGGTCCCCGTGCAGTCGATGGGCGCGGACGGCGCGTCCTCCG
  TGCACTGGTTCCGCAAAGGACTACGGCTCCACGACAACCCCGCGCTGTTAGCTGCCGTGCGCGGGGCGCG
  CTGTGTGCGCTGCGTCTACATCCTCGACCCGTGGTTCGCGGCCTCCTCGTCAGTGGGCATCAACCGATGG
  AGGTTCCTACTGCAGTCTCTAGAAGATCTGGACACAAGCTTAAGAAAGCTGAATTCCCGTCTGTTTGTAG
  TCCGGGGTCAGCCAGCTGATGTGTTCCCAAGGCTTTTCAAGGAATGGGGGGTGACCCGCTTGACCTTTGA
  ATATGACTCCGAACCCTTTGGGAAAGAACGGGATGCAGCCATTATGAAGATGGCCAAGGAGGCGGGTGTG
  GAGGTGGTGACTGAGAACTCTCACACCCTTTATGACTTAGACAGAATCATCGAACTGAATGGGCAGAAAC
  CACCCCTTACCTACAAGCGCTTTCAGGCTCTCATCAGCCGTATGGAGCTGCCCAAGAAGCCAGTGGGGGC
  TGTGAGCAGCCAGCATATGGAGAACTGCAGAGCTGAGATCCAGGAGAACCATGATGACACCTATGGCGTG
  CCTTCCTTAGAGGAACTGGGATTCCCCACAGAAGGACTTGGCCCAGCTGTTTGGCAAGGAGGAGAGACAG
  AAGCTCTGGTCCGCCTGGATAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCTCGGAT
  GAATGCCAATTCCTTGCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGCTGCCTCTCCTGC
  CGCCTCTTCTACTACCGCCTGTGGGACTTGTACAGAAAGGTGAAGAGGAACAGCACACCCCCCCTCTCCT
  TATTTGGACAACTCCTATGGCGAGAATTCTTCTATACAGCGGCCACCAACAACCCCAGGTTTGACCGAAT
  GGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGAAACCCCGAAGCCCTGGCCAAGTGGGCCGAG
  GGCAAGACAGGCTTCCCTTGGATTGACGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACC
  TGGCCCGGCACGCTGTGGCCTGCTTCCTCACCCGAGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCG
  GGTATTTGATGAGTTGCTCCTGGATGCAGATTTCAGCGTGAATGCAGGCAGCTGGATGTGGCTGTCCTGC
  AGTGCTTTCTTCCAACAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGACGCACGGACCCCAGTG
  GGGACTACATCCGGCGATACCTGCCCAAACTGAAAGGCTTCCCCTCTCGATATATCTATGAGCCCTGGAA
  TGCTCCCGAGTCGGTTCAGAAGGCCGCTAATTGCATCATTGGCGTGGACTACCCACGGCCCATCGTTAAC
  CACGCAGAGACTAGTCGGCTCAACATTGAGCGGATGAAGCAGATCTACCAACAGCTGTCACGATACCGGG
  GGCTCTGTCTGTGGGCATCTGTCCCTTCCTGTGTAGAAGACCTCAGTCACCCTGTGGCAGAGCCTGGTTC
  TAGCCAGGCTGGGAGCATCAGCAACACAGGCCCCAGACCACTGTCCAGTGGCCCAGCCTCCCCCAAACGC
  AAGCTGGAAGCAGCTGAGGAACCTCCAGGTGAAGAACTGAGCAAGCGGGCTAGAGTGACAGTGACTCAGA
  TGCCTGCCCAGGAGCCACCAAGCAAGGACTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133405
• Insert Size: 1785 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133405.1, NP_596896.1
• RefSeq Size: 3988 bp
• RefSeq ORF: 1785 bp
• Locus ID: 170917
• Cytogenetics: 3q24
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]