Dysf (NM_001107869) Rat Untagged Clone

CAT#: RN209414

Dysf (untagged ORF) - Rat dysferlin (Dysf), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dysf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN209414 representing NM_001107869
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTGCTGCCTGCTGGCAAGGGCCAGCAACCTCCCCAATGTGAAGAAGGACCGGCGCAGTGACCCTG
  TTGCCAGCCTGATTTTTCGAGAAGCCAAGATCCCACTCCAGGAGGTCCTTGCCACCCCCAGCCTATCTGC
  CAGCTTCAACGCACCTCTGCTGGACTCCAAGCAGCAACCCACAGGGGCCTCTCTGGTCCTGCAGGTATCC
  TACACACCGCCCCCAGGAGCTGCGCCCTTGTTCCCACCACCCGCTTCCCTGGCACCGTCCCCAACACTGC
  CTGACATGGACCTGGTGGCTGACACAGGAGGAGAGGAAGACACGGAAGACCAGGGCCTGACTGGAGATGA
  AGCTGAGCTCTTCCCAGATCCGAGTGTTGCTGTGGGCCCAGGGGGACCAACGACGCCCAAGAAGCCACCT
  TCCCATCCTCCTCCCCACTATCCAGGGGGGAAAAGGAAAAGAAGCTCTGCTCCACCTAGAAAGCTGCTGT
  CAGACAAGCCACAGGACTTCCAGATCAGAGTCCAGGTGATTGAGGGGCGTCAGCTGCCTGGGGTGAACAT
  CAAGCCTGTGGTCAAGGTCACAGCTGCTGGGCAGACCAAGCGGACTCGAATCCAGAAGGGAAATAGCCCA
  CTTTTCAATGAGACTCTTTTCTTCAACGTATTTGACTCTCCCTTGGAACTGTTTGATGAGCCCATCTTCA
  TCACGGTGGTAGACTCACGCTCCCTCAGGACAGATGCCCTCCTTGGCGAGTTCCGGATGGATGTGGGCAC
  CGTGTACAGAGAGCCCCGACATGCTTATCTCCGGAAGTGGCTGCTGCTCTCCGATCCCGATGACTTCTCT
  GCAGGGGCCAGAGGCTACCTCAAAGCCAGCCTGTGTGTGCTGGGGCCTGGGGACGAAGCTCCCCTAGACA
  AGAAAGACCCTTCTGAAGACAAAGAGGACATTGAAGGCAACCTGCTTAGGCCCACTGGTGTGGCCCTTCG
  TGGTGCCCATTTTTGCCTGAAGCTCTTCCGGGCTGAGGACTTACCACAGATGGACGATGCTGTGATGGAC
  AACGTCAAGCAAATCTTTGGCTTTGATAGCAACAAGAAGAACCTGGTGGACCCCTTTGTGGAAGTCAGCT
  TTGCTGGGAAAATGCTTTGCAGCAAGATCCTGGAGAAGACTGCTAACCCTCAGTGGAACCAGAACATCAC
  ATTGCCTGCGATGTTTCCCTCTATGTGTGAAAAAATGAGGATTCGTGTCATGGACTGGGACCGCCTCACT
  CACAATGACACTGTGGCTACCACCTACTTGGGTATGTCGAAAATCTCTGCCACTGGAGGAGAAATAGAAG
  AGGAGCCTGCAGGTGTACTCAAGAGTCCAGCCACTGACCTGGATGACAACCTGGGCTTCCTCCCCACTTT
  TGGGCCCTGCTATGTGAACCTCTATGGCAGTCCCCGAGAGTTCACGGGCTTCCCAGATCCTTATGCGGAG
  CTCAACACAGGAAAGGGAGAAGGTGTGGCATATAGGGGCAGGCTTCTGTTGTCCCTGGAGACCAAACTGG
  TGGAGCACAGTGAACAGAAGGTGGAAGACCTTCCTGCTGATGACATCTTGAGAGTGGAGAAGTACCTGCG
  GAGGCGCAAATATTCCCTCTTTGCTGCCTTCTACTCAGCCACAATGCTGCAGGATGTGGATGATGCCATC
  CAGTTCGAGGTCAGCATTGGGAACTACGGAAACAAGTTTGACACCACCTGCTTGCCGTTGGCCTCTACCA
  CCCAATACAGCCGGGCAGTCTTTGATGGATGCCACTACTATTATTTGCCCTGGGGCAACGTGAAGCCTGT
  GGTAGTACTGTCCTCATACTGGGAAGACATCAGCCATCGAGTCGAGACTCAGAACCAGCTCCTCAGGATC
  GCTGACCGACTGGAAGCTAACCTGGAGCAAGTCCACCTGGCTCTGAAGGCACAGTGTTCCTCTGAGGATG
  TGGACGCCCTGGTGGCTCAGTTGACAGATGAGCTCCTTGCAGACTGCAGCCAGCCCCTGTGTGACATCCA
  TGAAATACCTTCTGCCACCCACCTTGATCAGTACCTGTTCCGCCTCCGCTCCCGTCACCTGAGCCAGATC
  AAAGAAGCTGCCCTGGCCTTGAAGCTTGGCCACAGTGAGCTCTCCACTGCCCTGGAGCAGGCCGAGGACT
  GGCTTCTGCATCTCCGTGCCCTGGCTGAGGAGCCCCAGAACAGCCTGCCAGATATCATCATCTGGATGCT
  GCAGGGTGACAAGCGTGTGGCCTACCAACGGGTGCCTGCCCATGAAGTCCTCTTCTCCCGGCGGGGCCCC
  AGCTACTGCGGCAGGAACTGTGGGAATCTGCAGACCATCTTTCTGAAATATCCTATGGAAGGCATGCCTG
  GGCCCCGGATGCCGGTGCAGATCCGGATCAAGCTCTGGTTTGGGCTCTCAGTGGATGAGAAGGAATTCAA
  TCAGTTTGCAGAGGGGAAGCTCTCTGTCTTTGCTGAAACGTATGAGAACCAGACAAAGCTGGCGCTGGTC
  GGGAACTGGGGCACGACGGGCCTCACCTACCCCAAGTTTTCTGACGTCACAGGCAAGATCAAACTACCGA
  AGGATAGCTTCCGCCCCTCAGCTGGCTGGGCCTGGGCAGGAGACTGGTTCGTGTGTCCAGAGAAGACCCT
  GCTCCATGATGCTGACGCCGGTCATCTGAGCTTTGTGGAGGAGGTGTTTGAGAACCAGACTCGGCTACCT
  GGAGGCCAGTGGATCTATATGAGTGACAACTACACCGACGTGAATGGGGAGAAGGTGCTTCCAAAGGACG
  ATATTGAATGCCCGCTGGGTTGGAAGTGGGAAGATGAAGAATGGTCCACAGACCTCAATCGGGCTGTTGA
  TGAACAAGGCTGGGAGTACAGCATCACCATACCCCCAGACCGAAAGCCCAAACACTGGGTCCCTGTTGAG
  AAGATGTATTACACTCACAGGCGTCGACGCTGGGTCCGACTGCGCAGGAGGGACCTCAGCCAGATGGAGG
  CACTGAAGAAGCACAGGCAGGCAGAGGCTGAGGGCGAGGGCTGGGAATACGCCTCTCTCTTTGGCTGGAA
  GTTCCACCTGGAGTACCGCAAGACAGATGCCTTTCGCCGCCGCCGCTGGCGCCGCCGCATGGAGCCACTG
  GAGAAGACGGGGCCTGCAGCGGTGTTTGCCCTTGAGGGGGCCCTGGGCGGCATGGTGGATGACAAGAGCG
  AAGATTCCATGTCTGTGTCTACCCTGAGCTTTGGCGTTAACAGACCCACAATCTCCTGCATCTTTGACTA
  TGGGAACCGCTACCATCTGCGTTGCTACCTGTACCAGGCCCGGGACCTGCCCGCCATGGACAAAGACTCC
  TTCTCTGATCCCTATGCCATTGTCTCCTTCCTGCATCAAAGCCAGAAGACGGTGGTGGAGAAGAACACCC
  TGAACCCCACCTGGGATCAAACCCTCATCTTCTATGAGATAGAGATCTTTGGCGAGCCGGCCAGTGTCGC
  CGAGCGGCCACCCAGCATTGTGGTGGAGCTGTATGACCATGACACCTATGGCGCTGACGAGTTCATGGGC
  CGCTGTATTTGTCAGCCAAGCCTGGAACGGATGCCCCGCCTGGCCTGGTTCCCACTGACCAGGGGCAGCC
  AGCCTGCCGGAGAGCTGCTGGCAGCGTTCGAGCTTATCCAGAGAGAGAAGCCAGCCATCCATCACATCCC
  TGGCTTTGAGATGCACGAAACTTCAAGGATTTTGGATGAGACGGAGGACACAGACTTGCCTTACCCACCA
  CCTCAAAGGGAGGCCAACATCTACATGGTTCCCCAGAACATCAAGCCAGCTCTCCAGCGTACAGCCATCG
  AGATCCTGGCCTGGGGCCTTCGGAACATAAAGAGCTATCAGATGGCCAGCGTGTCTTCCCCCAGCCTCGT
  GGTGGAGTGTGGTGGGCAGACAGTGCAGTCCTGTGTCATCAGAAACCTTCGGAAGAACCCCAACTTTGAC
  GTCTGTACTCTGTTCATGGAAGTGATGTTACCCAGAGAGGACCTCTACTGTCCCCCTATTGTGGTCAAGG
  TCATTGATAATCGCCAGTTTGGCCGCAGGCCAGTTGTGGGTCAGTGCACCATTCGCTCCCTGGAGAACTT
  CCTGTGTGACCCGTACTCAGCAGAGAGTCCATCCCCGCAAGGTGGCCCAGATGACGTGAGCTTACTCAGC
  CCTGGGGAAGACGTGCTCATTGACATTGATGACAAGGAGCCTCTCATCCCTGTCCAGGAGGAGGAGTTCA
  TTGACTGGTGGAGCAAATTCTTTGCTTCTATCGGGGAGAGAGAAAAGTGTGGTTCCTACCTGGAGAAGGA
  TTTTGACACCCTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCCTTTGGAGGCCTGTCCGACTTT
  TGTAATACCTTCAAGCTCTACCGGGGCAAGACTCAGGAGGACACAGAAGATCCATCTGTCATCGGAGAAT
  TTAAGGGTCTCTTCAAAATTTATCCCCTCCCAGAAGATCCAGCCATCCCTATGCCCCCGAGACAATTCCA
  TCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGGATTTACATCATCCGAGCATTTGGCTTACAG
  CCCAAGGACCCCAATGGCAAGTGCGACCCTTACATCAAGATCTCTATAGGGAAGAAATCAGTGAGTGACC
  AGGATAACTACATCCCTTGTACCCTGGAGCCTGTATTTGGAAAGATGTTTGAACTCACCTGCACTCTACC
  TCTGGAGAAGGACTTGAAGATCACACTCTATGACTATGACCTGCTCTCCAAGGATGAGAAGATTGGGGAG
  ACAGTCATTGATCTGGAGAATAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGCCTTCCACAGACCTACT
  GTGTCTCTGGACCAAATCAGTGGAGAGATCAACTCCGCCCTTCCCAGCTCCTCCACCTCTTCTGCCAGCA
  GCACAGGGTCAAGGCCCCCGTGTACCGGACAGACCGAGTGACGTTTCAGGATAAGGAATACACCATTCAG
  GAGATAGAGGCTGGCAGACTCCCAAACCCACACCTGGGCCCGGTGGAGGAACGCTTAGCCCTGCATGTCC
  TTCAACAACAAGGCTTGGTCCCTGAGCATGTGGAGTCTCGGCCTCTGTACAGTCCTCTGCAGCCAGATAT
  TGAGCAGGGGAAGCTACAGATGTGGATTGACATATTTCCAAAGGTGCTGGGCCGGCCTGGACCTCCCTTC
  AACATCACCCCACGGAAAGCTCGAAGGTTTTTTCTGCGTTGTATTATTTGGAACACAAAAGATGTGATCT
  TGGATGACCTCAGCCTCACGGGGGAGAAGATGAGTGACATCTATGTGAAAGGCTGGATGGTGGGATTCGA
  AGAACACAAACAGAAGACAGATGTGCACTACCGTTCCCTGGGCGGGGAGGGCAATTTCAACTGGAGGTTC
  GTGTTTCCCTTCGACTATCTGCCCGCTGAGCAAGTCTGTGCTGTTGCCAAGAAGGATGCTTTCTGGAGAC
  TCGACAAGACAGAGAGCAAGATCCCAGCACGTGTGGTCTTCCAGATTTGGGACAATGACAAGTTCTCCTT
  TGATGACTTTCTGGGCTCCCTGCAGCTGGATCTCAACCGAATGCCCAAGCCAGCCAAGACGGCTGAGAAG
  TGCTCCTTGGACCAGCTGGATGACACCTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGAAGACAG
  TGAAAGGATGGTGGCCTTGTGTGACAGAGGAGGGCGCGAAGAAGATCCTGGCGGGGAAGCTGGAAATGAC
  CTTGGAGATCGTTGCAGAAAGTGAACATGAGGAGCGGCCTGCTGGCCAGGGTCGGGATGAACCCAACATG
  AATCCAAAGCTAGAAGATCCCAGGCGCCCTGACACCTCTTTCCTGTGGTTCACCTCACCATACAAGACCA
  TGAAGTTCATCTTGTGGCGACGATTCCGGTGTGCCATCATCCTCTTCATCATCCTCTTCATCCTCCTGCT
  CTTCCTGGGTGTCTTTGTCTACGCCTTCCCGAACTATGCTGCCATGAAGCTGGTGAAGCCCTTTAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001107869
• Insert Size: 6090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107869.1, NP_001101339.1
• RefSeq Size: 6319 bp
• RefSeq ORF: 6090 bp
• Locus ID: 312492
• Cytogenetics: 4q34