Ppm1b (NM_033096) Rat Untagged Clone

CAT#: RN208616

Ppm1b (untagged ORF) - Rat protein phosphatase 1B, magnesium dependent, beta isoform (Ppm1b), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ppm1b
• Synonyms: Pp2c2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208616 representing NM_033096
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCATTTTTGGATAAACCCAAAACTGAAAAGCACAATGCTCACGGTGCAGGGAACGGTCTGCGTT
  ACGGTCTGAGCAGTATGCAAGGATGGAGAGTAGAAATGGAAGACGCACACACTGCTGTTGTGGGAATTCC
  TCACGGCTTGGAGGACTGGTCGTTTTTTGCAGTCTATGATGGTCATGCTGGATCCCGAGTGGCAAATTAC
  TGTTCAACACATCTATTAGAACACATCACTACCAATGAAGACTTTAGGGCAGCTGACAAATCAGGCTTTG
  CTCTTGAGCCGTCAGTGGAAAATGTTAAGACTGGTATCCGAACTGGCTTTTTGAAAATTGATGAATATAT
  GCGTAACTTTTCAGACCTGAGGAACGGGATGGACAGGAGCGGCTCTACCGCAGTGGGCGTGATGATTTCA
  CCGACACACATCTACTTTATCAACTGCGGTGACTCGAGAGCTGTTCTGTGTAGGAATGGACAAGTCTGCT
  TTTCTACCCAGGATCACAAACCTTGTAATCCAATGGAAAAGGAGCGCATCCAAAATGCAGGAGGCAGTGT
  AATGATACAGCGTGTGAATGGGTCGTTAGCAGTGTCTCGTGCTCTGGGGGACTATGATTACAAGTGTGTT
  GATGGCAAGGGCCCCACAGAGCAGCTTGTTTCTCCAGAGCCTGAGGTTTATGAGATTCTAAGAGCAGAAG
  AGGATGAATTTGTCGTCCTGGCTTGTGATGGGATCTGGGATGTGATGAGCAATGAGGAGCTCTGTGAGTT
  TGTTAACTCTAGGCTTGAGGTGTCAGACGACCTGGAGAATGTGTGCAATTGGGTAGTGGACACTTGTTTA
  CATAAGGGAAGTCGAGATAACATGAGTATTGTGTTAGTTTGCTTTGCAAATGCCCCCAAGGTCTCAGATG
  AAGCCGTGAAAAGAGATTTAGAGTTGGACAAGCACTTGGAATCACGGGTGGAAGAAATCATGCAGAAGTC
  TGGAGAGGAAGGAATGCCTGATCTTGCCCATGTGATGCGCATTTTGTCTGCAGAAAATATCCCAAATTTA
  CCTCCCGGGGGAGGCCTCGCTGGCAAGCGCAATGTTATTGAAGCTGTTTATAGTAGACTTAATCCAAACA
  AAGACAATGATGGGGGCGCTGGCGATCTAGAAGACTCATTGGTAGCCTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033096
• Insert Size: 1173 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033096.2, NP_149087.1
• RefSeq Size: 3267 bp
• RefSeq ORF: 1173 bp
• Locus ID: 24667
• UniProt ID: P35815
• Cytogenetics: 6q12
• Gene Summary: Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.