Atp6v0a1 (NM_031604) Rat Untagged Clone

CAT#: RN208343

Atp6v0a1 (untagged ORF) - Rat ATPase, H+ transporting, lysosomal V0 subunit A1 (Atp6v0a1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Atp6v0a1
• Synonyms: Atp6n1; Atp6n1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208343 representing NM_031604
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGCTTTTCCGAAGCGAGGAGATGACCCTGGCCCAGCTCTTTCTCCAGTCCGAAGCTGCTTATT
  GTTGTGTCAGTGAATTAGGAGAACTTGGAAAGGTTCAGTTCCGGGATTTAAATCCAGATGTGAATGTTTT
  CCAGAGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAAACTCCGGTTTGTTGAGAAA
  GAGATAAGAAAAGCTAACATCCCAATTATGGACACTGGAGAGAACCCGGAGGTGCCCTTTCCCAGGGACA
  TGATCGACTTGGAGGCCAATTTCGAGAAGATCGAAAACGAACTGAAGGAAATCAACACTAACCAGGAAGC
  TCTAAAGCGAAACTTCCTGGAACTGACTGAATTAAAATTTATACTGCGCAAAACTCAGCAGTTTTTCGAT
  GAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCATCACTCTTGGAGCCAAACGAGATGGGAAGAGGCG
  CGCCTTTACGACTTGGCTTCGTAGCTGGTGTGATTAACCGGGAACGGATCCCGACCTTTGAGCGCATGCT
  TTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAGGCTGAAATCGAAAACCCGCTGGAGGATCCCGTG
  ACTGGCGACTACGTGCACAAGTCCGTGTTCATCATTTTTTTCCAAGGTGACCAGCTGAAAAACAGGGTCA
  AGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCCCTGTCCTGAGACTCCACAGGAGAGAAAAGAAAT
  GGCTTCCGGAGTCAATACCAGGATCGACGATCTCCAAATGGTTCTGAATCAGACAGAGGACCACCGCCAG
  AGGGTTCTGCAGGCGGCTGCTAAGAACATCCGGGTGTGGTTCATCAAGGTGCGGAAGATGAAAGCTATCT
  ACCATACCCTGAACCTCTGCAACATCGATGTGACGCAGAAGTGCCTGATCGCGGAAGTCTGGTGCCCCGT
  CACTGACCTGGACTCCATCCAGTTTGCACTGCGAAGGGGCACGGAGCACAGCGGTTCCACTGTCCCCTCC
  ATTCTGAACAGGATGCAGACAAACCAGACACCCCCGACGTATAACAAAACCAACAAGTTCACGCACGGCT
  TCCAGAACATAGTGGATGCTTATGGGATCGGCACTTACCGAGAAATTAACCCAGCTCCGTACACCGTCAT
  CACCTTCCCCTTTCTGTTTGCTGTGATGTTTGGAGATTTTGGGCACGGCATTTTGATGACTCTGTTTGCT
  GTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCCAGAAGAACGAGAATGAGATGTTTAGCATGGTGT
  TCAGCGGCCGATACATTATCCTTCTGATGGGACTGTTCTCCATCTACACTGGACTCATCTACAATGACTG
  CTTTTCCAAGTCTCTGAATATCTTTGGGTCATCATGGAGCGTACGGCCAATGTTCACCATAGGGAACTGG
  ACGGAGGAGACGCTCCTGGGGAGTTCTGTCCTCCAGCTGAACCCAGCTATCCCTGGAGTCTTTGGTGGCC
  CTTACCCATTTGGCATTGATCCGATTTGGAACATCGCAACCAACAAGCTGACCTTCCTCAACTCCTTCAA
  GATGAAGATGTCAGTTATTCTTGGGATCATCCACATGCTGTTCGGGGTCAGCCTGAGCCTTTTCAACCAC
  ATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCTTTATTCCCGAGATAATCTTCATGTCCTCGCTGT
  TTGGCTACCTGGTCATCCTCATCTTTTACAAGTGGACAGCCTACGATGCCCACTCGTCTAGAAATGCCCC
  GAGCCTCCTGATCCATTTCATAAACATGTTCCTCTTCTCCTACCCAGAGTCTGGTAACGCAATGCTGTAC
  TCTGGACAGAAAGGAATTCAGTGTTTCCTCATAGTGGTGGCAATGCTCTGTGTCCCCTGGATGCTGCTGT
  TTAAGCCGTTGATCCTTCGCCATCAGTACCTGAGGAAGAAGCATTTGGGAACTCTCAACTTTGGTGGGAT
  CAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAAATTATTCAGCATGACCAGCTCTCCACCCATTCA
  GAGGACGCAGAAGAGCCTACCGAGGACGAAGTGTTTGACTTTGGCGACACCATGGTCCACCAGGCCATCC
  ACACCATTGAGTACTGCTTGGGCTGCATCTCCAACACTGCGTCCTACCTGCGGCTCTGGGCCCTCAGCCT
  GGCCCATGCACAGCTCTCTGAAGTACTCTGGACCATGGTGATCCACATCGGCCTGCATGTCCGGAGTCTG
  GCAGGGGGACTGGGGCTGTTTTTCATCTTCGCTGCCTTTGCCACCCTGACCGTGGCCATCCTGCTGATCA
  TGGAAGGCCTCTCAGCCTTCCTCCACGCACTGCGATTACACTGGGTGGAATTCCAGAACAAATTCTACAC
  TGGGACTGGTTTCAAGTTCCTCCCCTTCTCCTTTGAGCACATTCGGGAAGGGAAGTTTGATGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031604
• Insert Size: 2517 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_031604.2, NP_113792.2
• RefSeq Size: 4006 bp
• RefSeq ORF: 2517 bp
• Locus ID: 29757
• UniProt ID: P25286
• Cytogenetics: 10q31
• Gene Summary: may play a role in coupling ATP hydrolysis to proton translocation [RGD, Feb 2006]