Klhl1 (NM_001106054) Rat Untagged Clone

CAT#: RN208329

Klhl1 (untagged ORF) - Rat kelch-like 1 (Drosophila) (Klhl1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Klhl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208329 representing NM_001106054
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGCTCTGGGCGAAAAGATTTCGATGTGAAGCACATCCTGCGACTCCGCTGGAAACTCTTCAGCC
  ATCCCTCACCAGCCTCCGGTAGCTCAGCGGGGGGCAGCTGCCTGCAACAGGACAGCGGGGGAGGCAGCTT
  TGAGCACTGGGGTCCTAGCCAGAGTCGGCTGCTCAAAAACCAGGAAAAGGGCAGTGTGAGCGCTTTCTGG
  AAAAAACCTTCTTCCTCTTCGACATCATCATCATCCTCGTCCTCTGCTTCATCCTCTCCTTTCAGTCCGC
  TGAATGGCACCTTACTACCAGTTGCCACGAGGCTGCAACAAGGGGCTCCTGGGCAGGGCACCCAGCAGCC
  CGCCAGGACTCTTTTCTACGTGGAGTCCCTAGAGGAGGAGGTAGTGACAGGCATGGACTTTCCTGGACCA
  CAGGACAAAGGATTGGCCCTGAAAGAGCTCCAAGCAGAGCCTGCCAGCTCTATCCAGGCAACAGGTGAAG
  GATGTGGACACAGGTACAGTAAGTTCCTACCAAGCAGGTTGACAACGACCAATCATTCACTGACACCTCA
  AAGTGATTTGGACTCCAGTAGCTCTGAAGAATTCTATCAAGCTGTTCACCATGCTGAGCAATCATTTAGG
  AAGATGGAGAATTATCTAAAGCAACAGCAGCTGTGTGATGTCATCCTGATAGTGGGGAATCGAAAGATAC
  CTGCTCATAGGCTTGTCCTGAGCTCTGTCTCTGACTACTTTGCTGCCATGTTTACAAGCGATGTCTGTGA
  AGCCAAGCAAGAGGAGATCAAGATGGAAGGCATAGACCCAAATGCACTCTGGGATCTTGTTCAATTTGCA
  TATACAGGTTGCTTGGAATTAAAAGAGGACACTATTGAAAACCTTCTTGCAGCCGCGTGCCTTCTCCAGC
  TCCCTCAAGTGGTCGAAGTGTGTTGTCACTTCCTCATGAAACTTCTTCATCCATCCAACTGCTTAGGAAT
  CCGGGCATTTGCAGACGCTCAAGGATGTATTGAATTAATGAAGGTGGCACACAGCTACACCATGGAAAAC
  ATAATGGAAGTAATCAGAAATCAAGAGTTTTTACTCCTTCCAGCCGAAGAGCTCCATAAACTACTGGCCA
  GTGATGACGTAAATGTGCCTGATGAAGAGACCATCTTCCATGCATTAATGATGTGGGTCAAGTACGACAT
  GCAGCGGAGATGCGGCGACCTGAGTATGCTCCTCGCCTTCATAAGACTGCCACTGCTTCCACCACAGATA
  CTGGCTGACCTTGAGAATCATGCACTATTCAAGAATGATTTAGAATGTCAAAAACTGATTCTGGAAGCAA
  TGAAATACCATCTATTGCCAGAAAGAAGAACTTTAATGCAAAGCCCAAGAACTAAGCCCAGAAAATCTAC
  AGTTGGAACTCTTTATGCGGTGGGGGGAATGGATAATAACAAAGGTTGTATTCAGTTGGGGGCCGAGATG
  GAAGTTCTTGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001106054
• Insert Size: 1485 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001106054.1, NP_001099524.1
• RefSeq Size: 2703 bp
• RefSeq ORF: 1485 bp
• Locus ID: 290426
• Cytogenetics: 15q21