Nkap (NM_001024872) Rat Untagged Clone

CAT#: RN207954

Nkap (untagged ORF) - Rat NFKB activating protein (Nkap), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nkap
• Synonyms: 2610020o08rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207954 representing NM_001024872
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTGTGTCGGGCTCGCGTAGCCCTGTACGGGAGGCCTCCGGGGGAAAGCGTCGCAGTTCGTCGA
  GGAGCCCCAAATCCATCAAATCTTCCCGCTCCCCGCGATGTCGCCGGTCTCGCTCGCGCTCTTGTTCTCG
  GTTCGGTGACCGGAATGGCCTCAGCCATTCGCTGAGTGGCTTCAGCCAAAGCTCTCGAAACCAATCTTAT
  CGCTCCCGCTCTCGATCGCGTTCTCGAGAACGGCCTTCTGCACAGCGGAGTGCCCCGTTCGCGTCTTCAT
  CCTCGTCCGCCTATTATGGCGGCTACTCGCGTCCCTACGGGGGTGACAAGCCATGGCCTAGCCTGCTGGA
  CAAGGAAAGGGAGGAGAGTCTGCGGCAGAAGAGATTAAGTGAAAGAGAGAGGATTGGAGAATTGGGAGCT
  CCTGAAGTTTGGGGACTTTCCCCAAAGAATCCTGAACCAGACTCTGATGAACATACACCAGTAGAGGATG
  AAGAACCAAAGAAAAGCACTACTTCAGCATCTAGTTCGGAAGATGATAAGAAGAAGAAAAGGAAATCTAG
  TCGTTCAAAAGAAAGAGCCAAGAAAAAGAGAAAGAAAAAGTCATCTAAAAGAAAACATAAGAAGTATTCT
  GAAGATAGCGACAGTGACTCAGAGTCTGACACCGACTCTAGTGATGAAGATTCAAAAAGAAGAGCAAAGA
  AAGCTAAGAAAAAGGAAAAGAAGAAGAAACGAAGAGGGAAGAAATACAAGAAAAAGAAGTCTAAGAAAAA
  CAGAAAGGAGTCCAGTGATTCAAGCTCTAAAGAGTCCCAAGAAGAATTTCTAGAGAATCCGTGGAAGGAT
  CGATCAAAGACAGAAGAACCATCAGATCTGATTGGCCCAGAGGCTCCCAAAACACTTGCCTCCCAAGATG
  ATAAGCCCTTGAACTATGGCCACGCCCTGTTACCTGGTGAAGGTGCAGCTATGGCTGAATATGTAAAAGC
  TGGCAAGCGTATCCCGAGAAGAGGTGAAATCGGCTTAACAAGTGAAGAGATCGCATCATTCGAATGTTCC
  GGTTATGTAATGAGCGGCAGCAGGCATCGCCGAATGGAGGCTGTCCGACTTCGGAAAGAGAACCAGATCT
  ACAGTGCCGATGAGAAGAGAGCCCTTGCCTCCTTTAACCAAGAAGAAAGACGGAAGAGGGAGAACAAGAT
  TCTGGCCAGCTTTCGCGAGATGGTGTACAGAAAGACCAAAGGGAAGGATGACAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024872
• Insert Size: 1248 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024872.1, NP_001020043.1
• RefSeq Size: 1466 bp
• RefSeq ORF: 1248 bp
• Locus ID: 298342
• UniProt ID: Q4V7C9
• Cytogenetics: Xq35
• Gene Summary: Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter (By similarity).[UniProtKB/Swiss-Prot Function]