Atg4a (NM_001126298) Rat Untagged Clone

CAT#: RN207459

Atg4al1 (untagged ORF) - Rat similar to Cysteine protease ATG4A (Autophagy-related protein 4 homolog A) (Autophagin-2) (Autophagy-related cysteine endopeptidase 2) (AUT-like 2 cysteine endopeptidase, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Atg4a
• Synonyms: Agt4a; Atg4al1; RGD1588268
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207459 representing NM_001126298
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCAGTTATGTCCAAGTATGAAAACCAGATTATTATTTTCACTGAATACCTAGAAGAATTCCCAG
  ATACAGATGAGCTAGTATGGATCCTGGGGAAGCCACATCTCCTTAAGACAGAAAAATCTAAGCTGTTGTC
  TGATATAAGTGCTCGTCTATGGTTTACATACAGAAGGAAATTTTCACCGATTGGGGGAACAGGCCCTTCG
  TCGGATGCTGGCTGGGGATGTATGCTGCGCTGTGGGCAGATGATGCTGGCTCAAGCCCTCATCTGTAGAC
  ACTTGGGAAGGGATTGGAACTGGGAGAGACAAAAAGAACAACCCAAAGAATACCAAAGGATACTGCAGTG
  TTTCCTAGATAGAAAAGACTGTTGCTATTCAATCCATCAGATGGCACAAATGGGTGTAGGAGAAGGGAAA
  TCAATTGGCGAATGGTTTGGACCAAATACAGTTGCACAGGTGATAAAAAAACTTGCTTTATTTGATGAAT
  GGAATTCCTTGGCTGTTTATGTTTCAATGGATAACACAGTGGTCATTGAAGATATCAAGAAAATGTGCTG
  TGTTCTTCCTGTGGGTGCTGCTGACACAGCTGGTGAGAGCCCTCCTGATTCTCTGATTGCTTCCAGTCAG
  AGTAAGGGCACCTCTGCCCCGTGCCTAGCCTGGAAACCCCTGCTGCTCATTGTGCCCCTTCGCCTGGGCA
  TAAACCAAATCAACCCTGTGTATATTGAAGCATTCAAAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGC
  TTTAGGAGGAAAGCCAAATAACGCCTATTATTTCATAGGATCCTTAGGTGACGAGCTCATTTTCTTGGAT
  CCTCACACAACCCAGACCTTCGTTGATACTGAAGAGAGTGGACTAGTAGACGACCACACTTTCCATTGCC
  TGCAGTCTCCACAGCGGATGAGTATCCTGAACTTGGATCCTTCTGTGGCCTTGGTGGGTCAAGGAGCTTT
  TATGGGATTTTTCTGCAAAGAAGAGAAAGACTTTGATAATTGGTGTAGTCTTGTTCAGAAGGAAATTCTA
  AAGGAGAATTTGAGGATGTTTGAATTAGTTCAGAAACATCCATCACACTGGCCTCCCTTTGTACCTCCAG
  CCAAGCCAGAAGTGACAACCACAGGGGCAGAATTCATAGACTCTACCGAACAACTGGAGGACTTCGACCT
  GGAGGAAGATTTTGAGATTCTGAGTGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001126298
• Insert Size: 1221 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001126298.1, NP_001119770.1
• RefSeq Size: 2171 bp
• RefSeq ORF: 1221 bp
• Locus ID: 678769
• Cytogenetics: Xq33