Nell1 (NM_031069) Rat Untagged Clone

CAT#: RN206967

Nell1 (untagged ORF) - Rat NEL-like 1 (chicken) (Nell1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nell1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206967 representing NM_031069
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGATGGATGTGATTTTAGTTTTGTGGTTCTGTGTATGCACCGCCAGGACAGTGTTGGGCTTTGGGA
  TGGACCCTGACCTTCAGCTGGACATCATCTCAGAGCTCGACCTGGTGAACACCACCCTGGGAGTCACGCA
  GGTGGCTGGACTGCACAACGCCAGTAAAGCATTTCTATTTCAAGATGTACAGAGAGAGATCCATTCGGCC
  CCTCACGTGAGTGAGAAGCTGATCCAGCTATTCCGGAATAAGAGCGAGTTCACCTTTTTGGCTACAGTGC
  AGCAGAAACCATCCACCTCAGGGGTGATACTGTCCATCCGGGAGCTGGAGCACAGCTATTTTGAACTGGA
  GAGCAGTGGCCCAAGAGAAGAGATACGCTACCATTACATACATGGTGGAAAGCCCAGGACTGAGGCCCTT
  CCCTACCGCATGGCAGACGGACAATGGCACAAGGTCGCGCTGTCAGTGAGCGCCTCTCACCTCCTGCTCC
  ACATCGACTGCAATAGGATTTACGAGCGTGTGATAGACCCTCCGGAGACCAACCTTCCTCCAGGAAGCAA
  TCTGTGGCTTGGGCAACGTAACCAAAAGCATGGCTTTTTCAAAGGAATCATCCAAGATGGTAAGATCATC
  TTCATGCCGAATGGTTTCATCACACAGTGTCCCAACCTCAATCGCACTTGCCCAACATGCAGTGACTTCC
  TGAGCCTGGTTCAAGGAATAATGGATTTGCAAGAGCTTTTGGCCAAGATGACTGCAAAACTGAATTATGC
  AGAGACGAGACTTGGTCAACTGGAAAATTGCCACTGTGAGAAGACCTGCCAAGTGAGTGGGCTGCTCTAC
  AGGGACCAAGACTCCTGGGTGGATGGTGACAACTGTGGGAACTGCACGTGCAAAAGTGGTGCCGTGGAGT
  GCCGCAGGATGTCCTGTCCCCCGCTCAACTGTTCCCCGGACTCACTTCCTGTGCACATTTCCGGCCAGTG
  TTGTAAAGTTTGCAGACCAAAATGTATCTATGGAGGAAAAGTTCTTGCTGAGGGCCAGCGGATTTTAACC
  AAGACCTGCCGGGAATGTCGAGGTGGAGTCTTGGTAAAAATCACAGAAGCTTGCCCTCCTTTGAACTGCT
  CAGCAAAGGATCATATTCTTCCAGAGAATCAGTGCTGCAGGGTCTGCCCAGGTCATAACTTCTGTGCAGA
  AGCACCTAAGTGCGGAGAAAACTCGGAATGCAAAAATTGGAATACAAAAGCAACCTGTGAGTGCAAGAAT
  GGATACATCTCTGTCCAGGGCAACTCTGCATACTGTGAAGATATTGATGAGTGTGCAGCTAAAATGCACT
  ATTGTCATGCCAACACCGTGTGTGTCAACTTGCCGGGGTTGTATCGCTGTGACTGCGTCCCAGGGTACAT
  CCGTGTGGATGACTTCTCTTGTACGGAGCATGATGATTGTGGCAGCGGACAACACAACTGCGACAAAAAT
  GCCATCTGTACCAACACAGTCCAGGGACACAGCTGCACCTGCCAGCCGGGTTACGTGGGAAATGGCACCA
  TCTGCAAAGCATTCTGTGAAGAGGGTTGCAGATACGGAGGTACCTGTGTGGCTCCTAACAAGTGTGTCTG
  TCCTTCTGGATTCACGGGAAGCCACTGTGAGAAAGATATTGATGAATGCGCAGAGGGATTCGTTGAATGC
  CACAACTACTCCCGCTGTGTTAACCTGCCAGGGTGGTACCACTGTGAGTGCAGAAGCGGTTTCCATGACG
  ATGGGACCTACTCACTGTCCGGGGAGTCCTGCATTGATATCGATGAATGTGCCTTAAGAACTCACACTTG
  TTGGAATGACTCTGCCTGCATCAACTTAGCAGGAGGATTTGACTGCCTGTGTCCCTCTGGGCCCTCCTGC
  TCTGGTGACTGTCCCCACGAAGGAGGGCTGAAGCATAATGGGCAGGTGTGGATTCTGAGAGAAGACAGGT
  GTTCAGTCTGTTCCTGCAAGGATGGGAAGATATTCTGCCGGCGGACAGCTTGTGATTGCCAGAATCCAAA
  TGTTGACCTTTTTTGCTGCCCAGAGTGCGATACCAGGGTCACCAGCCAATGTTTAGATCAAAGTGGACAG
  AAGCTCTATCGAAGTGGAGACAACTGGACCCACAGCTGCCAGCAGTGCCGATGTCTGGAAGGAGAGGCAG
  ACTGCTGGCCTCTGGCTTGCCCTAGTTTGGGCTGTGAATACACAGCCATGTTTGAAGGGGAGTGTTGTCC
  CCGATGTGTCAGTGACCCCTGCCTGGCTGGTAATATTGCCTATGACATCAGAAAAACTTGCCTGGACAGC
  TTTGGTGTTTCGAGGCTGAGCGGAGCCGTGTGGACAATGGCTGGATCTCCTTGTACAACCTGCAAATGCA
  AGAATGGGAGAGTCTGCTGCTCTGTGGATCTGGAGTGTATTGAGAATAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031069
• Insert Size: 2433 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031069.1, NP_112331.1
• RefSeq Size: 2915 bp
• RefSeq ORF: 2433 bp
• Locus ID: 81733
• UniProt ID: Q62919
• Cytogenetics: 1q22
• Gene Summary: interacts with and phosphorylated by protein kinase C betaI (PKCbetaI) and is involved in modulation of osteoblast differentiation; overexpression of human homolog is associated with craniosynostosis [RGD, Feb 2006]