Atp7b (NM_012511) Rat Untagged Clone

CAT#: RN206811

Atp7b (untagged ORF) - Rat ATPase, Cu++ transporting, beta polypeptide (Atp7b), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Atp7b
• Synonyms: Hts; PINA; Wd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206811 representing NM_012511
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAACAGGAGAGAAAGGTCACAGCCAAAGAGGCCAGTCGGAAAATCTTATCTAAACTTGCTTTGC
  CCACACGACCGTGGGGACAATCAATGAAGCAGAGCTTCGCCTTCGATAATGTTGGCTATGAAGGGGGCCT
  GGACAGCACCTGCTCATCCTCAACTACCACCGGTGTGGTTAGCATCCTGGGCATGACTTGTCATTCTTGC
  GTCAAGTCCATCGAGGACAGGATCTCCAGTCTGAAAGGCATTGTGAGCATCAAGGTTTCTCTGGAGCAGG
  GCAGCGCCACTGTCAAATATGTACCGTCAGTCTTGAACCTGCAGCAGATTTGCCTTCAGATTGAGGACAT
  GGGCTTTGAGGCCAGCGCTGCAGAAGGAAAGGCTGCCTCCTGGCCTTCCAGGTCTTCCCCAGCCCAGGAG
  GCAGTGGTCAAGCTCCGGGTAGAGGGCATGACCTGTCAGTCCTGTGTCAGCTCCATCGAAGGCAAGATCC
  GGAAGCTGCAAGGGGTTGTGAGAGTCAAAGTCTCCCTAAGCAACCAAGAGGCAGTCATTACATATCAGCC
  TTACCTCATTCAACCCGAAGACCTCAGGGACCACATCTGCGACATGGGATTCGAAGCTGCCATCAAGAAC
  AGAACAGCTCCCTTAAGGCTGGGACCAATTGATATCAACAAGTTAGAAAGCACTAACCTAAAGAGAGCAG
  CAGTCCCTCCTATCCAGAATTCCAATCATTTGGAGACCCCGGGGCACCAGCAGAACCACCTGGCCACCCT
  CCCACTAAGAATAGACGGGATGCACTGTAAATCATGTGTTTTGAATATCGAAGGAAATATAGGCCAACTT
  CCAGGGGTTCAAAATATTCATGTGTCCTTGGAGAACAAAACCGCCCAAGTACAGTATGACTCTTCTTGTA
  TCACCCCCTTGTTCCTACAGACAGCCATCGAGGCACTACCACCTGGGTACTTTAAAGTATCCCTTCCCGA
  TGGCCTAGAGAAGGAGAGTGGATCTTCCAGTGTCCCCTCCCTTGGCTCCTCCCAGAGACAGCAGGAGCCA
  GGCCCATGCAGGACTGCGGTACTCACCATCACTGGCATTCCCCGTGACTCGTCTGTTCAGCCCATGGAAG
  ACATGCTGTCCCAGATGAAGGGTGTGCAGCAAATAGACATCTCTTTGGCAGAGGGGACTGGAGCAGTTCT
  TTACGATCCCTCAGTAGTTAGCTCGGATGAACTCCGGACGGCTGTAGAAGACATGGGCTTTGAGGTGTCA
  GTGAATCCCGAAAACATTACTACTAACCGTGTCAGCTCTGGGAATTCTGTGCCACAAGCCGTGGGTGATT
  CACCAGGGTCTGTGCAAAATATGGCTTCTGACACTAGAGGACTCCTCACACACCAAGGCCCTGGCTACTT
  GTCAGACAGCCCACCATCCCCTGGAGGAACAGCATCACAGAAGTGCTTTGTACAGATCAAAGGCATGACC
  TGTGCGTCCTGTGTGTCTAACATAGAAAGGAGTCTGCAGAGACATGCCGGTATTCTCTCCGTGTTGGTCG
  CCTTGATGTCGGGAAAGGCAGAGGTCAAGTATGACCCAGAGGTCATCCAGTCTCCCAGGATAGCTCAGCT
  CATCGAGGACCTGGGCTTCGAAGCAGCAATCATGGAGGACAACACAGTCTCTGAAGGTGACATCGAACTG
  ATTATCACAGGGATGACCTGCGCTTCCTGTGTTCACAACATAGAATCTAAGCTCACAAGGACAAATGGCA
  TCACTTACGCCTCTGTGGCCCTCGCCACCAGCAAAGCCCATGTGAAGTTTGACCCTGAAATCATTGGTCC
  ACGTGACATCATCAAGGTCATCGAGGAAATCGGCTTTCATGCTTCCCTGGCCCACAGAAACCCCAACGCT
  CATCACTTGGACCACAAGACGGAAATAAAACAGTGGAAGAAATCTTTCCTGTGCAGCCTGGTGTTTGGCA
  TCCCCGTCATGGGCTTGATGATCTACATGCTAATCCCCAGCAGTAAGCCCCACGAGACCATGGTCCTGGA
  CCACAACATCATTCCAGGACTGTCCGTTCTAAACCTCATTTTCTTCATCTTGTGTACCTTCGTCCAATTC
  CTGGGTGGGTGGTACTTCTATGTCCAAGCCTACAAATCGCTGAGACACAAGTCAGCCAACATGGATGTGC
  TCATCGTACTCGCCACGACCATTGCCTATGCCTACTCCCTGGTCATCCTGGTGGTTGCCATAGCTGAAAA
  GGCGGAGAAGAGCCCAGTGACCTTCTTTGACACACCCCCCATGCTCTTCGTCTTCATCGCCCTGGGACGG
  TGGCTGGAGCACGTGGCAAAGAGCAAAACTTCAGAAGCCCTCGCAAAACTCATGTCACTCCAAGCCACAG
  AAGCCACAGTTGTGACCCTGGGAGAGGACAACTTAATCCTCAGAGAGGAGCAAGTGCCCATGGAGCTGGT
  GCAGCGAGGTGACATCATCAAGGTTGTCCCTGGGGGCAAGTTCCCAGTGGACGGGAAAGTCCTGGAAGGC
  AACACCATGGCAGATGAGTCCCTCATCACAGGAGAGGCCATGCCTGTCACCAAGAAACCCGGGAGCATAG
  TGATTGCTGGCTCTATAAATGCTCATGGCTCTGTGCTCATTAAAGCTACCCATGTGGGCAATGACACTAC
  TTTGGCTCAGATTGTCAAGTTGGTGGAAGAGGCCCAGATGTCAAAGGCTCCCATTCAGCAGCTGGCTGAC
  CGGTTCAGTGGATATTTCGTCCCATTTATCATCATTATTTCAACCTTAACATTGGTGGTGTGGATCATCA
  TCGGCTTTGTCGATTTTGGTATTGTTCAGAAGTACTTTCCTAGCCCTAGCAAGCATATCTCACAGACAGA
  GGTGATCATCCGCTTTGCCTTCCAGACGTCCATCACCGTCCTGTGCATCGCCTGCCCCTGCTCCCTCGGG
  CTGGCCACACCCACAGCAGTTATGGTGGGCACTGGGGTGGCTGCCCAGAACGGCGTCCTAATCAAGGGAG
  GGAAGCCTCTGGAGATGGCACACAAGATAAAGACCGTTATGTTTGACAAAACGGGCACCATTACCCACGG
  GGTCCCCAGAGTCATGCGGTTTCTGCTGCTTGTGGACGTGGCTACCCTATCCCTCAGGAAGGTTCTGGCT
  GTGGTGGGCACCGCAGAGGCCAGCAGTGAGCACCCCTTAGGCGTGGCCGTCACTAAATACTGCAAAGAGG
  AACTCGGGACGGAGACCCTGGGGTACAGCACGGACTTCCAGGCAGTGCCAGGGTGTGGAATTAGCTGCAA
  AGTTAGCAACGTGGAAAGTATCCTGGCTCACAGAGGTCCAACCGCTCACCCGATTGGGGTTGGCAACCCT
  CCCATAGGAGAAGGTACAGGTCCCCAGACTTTCTCTGTGCTGATTGGAAACCGGGAATGGATGAGGCGCA
  ATGGTTTAACCATCTCCAGTGACATCAGTGACGCCATGACAGATCATGAAATGAAAGGACAGACGGCCAT
  CCTGGTGGCCATTGATGGTGTGCTGTGCGGGATGATCGCCATTGCAGATGCTGTTAAACCAGAGGCTGCC
  CTGGCTATCTACACCCTGAAAAGCATGGGCGTGGATGTGGCTCTGATCACAGGGGACAACCGGAAGACAG
  CCAGAGCCATTGCCACTCAGGTTGGCATCAACAAAGTCTTTGCTGAGGTACTGCCTTCTCACAAGGTGGC
  CAAGGTCCAGGAGCTTCAGAACAAAGGGAAAAAAGTCGCCATGGTGGGAGACGGGGTGAACGACTCCCCA
  GCCTTGGCCCAGGCTGACGTGGGCATTGCTATTGGGACTGGGACAGATGTCGCCATCGAAGCAGCCGACG
  TGGTCCTTATAAGAAATGACTTACTGGACGTGGTGGCCAGCATTCATCTCTCCAAGAGGACCGTCCGGAG
  GATCCGGGTCAATCTGGTGCTGGCGTTGATTTATAACATGGTTGGGATACCCATTGCTGCAGGTGTCTTC
  ATGCCCATTGGCATCGTGCTGCAGCCATGGATGGGCTCAGCGGCCATGGCCGCCTCCTCTGTGTCCGTGG
  TGCTCTCCTCTCTTCAGCTCAAGTGCTACAGAAAGCCCGACCTAGAGAGATATGAGGCACAGGCCCATGG
  ACGCATGAAGCCTCTGAGTGCATCCCAAGTCAGCGTGCACGTTGGCATGGATGACCGGCGGCGGGATTCT
  CCCAGGGCCACACCCTGGGACCAGGTCAGCTACGTGAGCCAAGTCTCTCTGTCTTCCCTGACGTCAGACA
  GATTGTCTCGGCATGGCGGTATGGCAGAGGATGGTGGAGACAAATGGTCCCTGCTCCTGAGTGACAGGGA
  TGAAGAGCAGTGCATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012511
• Insert Size: 4359 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012511.2, NP_036643.2
• RefSeq Size: 5506 bp
• RefSeq ORF: 4359 bp
• Locus ID: 24218
• Cytogenetics: 16q12.5
• Gene Summary: plays a role in copper ion transport; partial gene deletion in the Long-Evans Cinnamon (LEC) strain is a model for Wilson disease [RGD, Feb 2006]