Npr1 (NM_012613) Rat Untagged Clone

CAT#: RN205931

Npr1 (untagged ORF) - Rat natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) (Npr1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Npr1
• Synonyms: Anpra; Gca; NPR-A; Npra
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205931 representing NM_012613
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGCTCCCGACGCGTCCGTCCGCGCCTAAGGGCGCTGCTGCTGCTGCCGCCGCTTCTGCTACTCC
  GGGGCGGCCACGCGAGCGACCTGACCGTGGCTGTGGTGCTGCCGCTGACCAACACCTCGTACCCGTGGTC
  CTGGGCGCGTGTAGGGCCGGCCGTGGAACTGGCTCTCGCGCGGGTGAAGGCTCGGCCGGACTTGCTGCCG
  GGTTGGACGGTCCGCATGGTGCTGGGCAGCAGTGAGAACGCGGCGGGCGTCTGCTCGGACACCGCCGCAC
  CGCTGGCCGCGGTGGACCTCAAGTGGGAGCACAGCCCCGCGGTGTTCCTGGGCCCCGGCTGCGTCTACTC
  CGCTGCCCCGGTGGGGCGCTTCACCGCGCACTGGCGGGTGCCGCTGCTGACCGCCGGCGCCCCGGCTCTG
  GGCATCGGGGTCAAGGATGAGTATGCGCTAACCACCCGCACAGGACCCAGCCATGTCAAGCTGGGCGATT
  TCGTGACGGCGCTGCATCGACGGCTGGGCTGGGAGCACCAGGCGCTGGTGCTCTATGCAGATCGGCTGGG
  CGACGACCGGCCTTGCTTCTTCATAGTGGAGGGGCTGTACATGCGGGTGCGTGAACGCCTCAACATCACA
  GTGAATCACCAGGAGTTCGTCGAGGGCGACCCGGACCACTACCCCAAGCTACTGCGGGCCGTGCGGCGAA
  AGGGCAGAGTTATCTACATCTGCAGTTCTCCGGATGCCTTCAGGAATCTGATGCTTCTGGCCCTGAACGC
  TGGCCTGACTGGGGAGGACTATGTTTTCTTCCACCTGGATGTGTTTGGGCAAAGCCTTAAGAGTGCTCAG
  GGCCTTGTTCCCCAGAAACCCTGGGAAAGAGGAGATGGGCAGGACAGGAGTGCCCGCCAGGCCTTTCAGG
  CTGCCAAAATTATTACTTACAAAGAGCCTGATAATCCTGAGTACTTGGAATTCCTGAAGCAGCTGAAACT
  CTTGGCTGACAAGAAGTTCAACTTCACCGTGGAGGATGGCCTGAAGAATATCATCCCAGCCTCCTTCCAC
  GACGGGCTCCTGCTCTATGTCCAGGCAGTGACAGAGACTCTGGCACAGGGGGGAACTGTCACAGATGGAG
  AGAACATCACTCAGCGGATGTGGAACCGAAGCTTCCAAGGTGTGACAGGATACCTGAAAATTGATAGAAA
  CGGAGATCGGGACACCGATTTCTCTCTCTGGGATATGGATCCAGAGACGGGTGCCTTCAGGGTTGTCCTG
  AACTATAATGGTACTTCCCAGGAGCTAATGGCTGTGTCAGAACACAAATTATACTGGCCTCTGGGATATC
  CACCTCCTGACGTCCCTAAATGTGGCTTTGACAATGAGGACCCAGCCTGCAACCAAGACCACTTTTCCAC
  ACTGGAGGTTCTGGCTTTGGTGGGCAGCCTCTCTCTGATTAGCTTTCTGATTGTGTCTTTCTTCATATAC
  AGGAAGATGCAGCTGGAAAAGGAGCTGGTCTCAGAGTTGTGGCGGGTGCGCTGGGAGGACTTGCAGCCCA
  GCAGCCTGGAGAGGCATCTTCGGAGCGCTGGCAGCCGGCTGACCCTGAGTGGGCGAGGCTCCAATTATGG
  CTCCCTGCTAACCACCGAGGGCCAGTTCCAAGTCTTTGCCAAGACAGCATACTATAAGGGCAACCTTGTG
  GCTGTGAAACGTGTGAACCGGAAACGCATTGAGTTGACACGAAAAGTCCTGTTTGAACTTAAACATATGC
  GGGATGTGCAGAATGAGCACTTGACAAGATTTGTGGGTGCTTGTACCGACCCCCCCAACATCTGTATCCT
  CACAGAGTACTGTCCCCGTGGAAGCCTACAGGACATTCTAGAGAATGAGAGTATCACCCTGGACTGGATG
  TTTCGGTACTCGCTCACCAATGACATTGTCAAGGGAATGCTCTTTCTACACAATGGGGCCATTTGTTCCC
  ATGGGAACCTCAAGTCATCCAACTGTGTGGTAGACGGGCGCTTCGTGTTAAAGATCACAGACTACGGTCT
  TGAGAGCTTCAGAGACCCGGAGCCAGAGCAAGGACACACCCTCTTTGCCAAAAAATTGTGGACGGCACCT
  GAGCTCCTGCGAATGGCTTCGCCACCTGCCCGTGGCTCCCAAGCTGGGGATGTGTACAGCTTTGGTATCA
  TCCTGCAGGAGATTGCCCTAAGAAGTGGGGTCTTCTATGTGGAAGGTTTGGACCTCAGCCCAAAAGAGAT
  CATTGAGCGTGTGACTCGGGGTGAGCAGCCCCCATTCCGACCCTCCATGGATCTGCAGAGCCACCTGGAG
  GAACTGGGGCAGCTGATGCAGCGGTGCTGGGCAGAGGACCCACAGGAGCGGCCACCCTTTCAGCAGATTC
  GCCTGGCGCTGCGCAAGTTCAACAAGGAGAACAGCAGCAACATCCTGGACAACCTGCTGTCACGCATGGA
  GCAGTATGCTAACAACCTGGAGGAACTGGTAGAGGAGAGAACACAAGCTTATCTGGAGGAGAAGCGCAAA
  GCTGAGGCCTTGCTTTACCAGATTCTGCCTCACTCCGTGGCTGAGCAGCTGAAGAGAGGCGAGACAGTCC
  AGGCTGAGGCCTTTGATAGTGTTACCATCTACTTCAGTGATATTGTGGGCTTTACAGCTCTTTCAGCAGA
  AAGCACACCCATGCAGGTGGTGACTCTGCTCAATGATCTGTACACCTGTTTTGATGCTGTCATAGACAAC
  TTTGATGTGTACAAGGTGGAGACCATTGGTGATGCTTACATGGTGGTGTCAGGGCTCCCAGTGCGGAATG
  GACAACTCCACGCCCGAGAGGTGGCCCGAATGGCACTTGCACTACTGGATGCTGTGCGCTCCTTCCGCAT
  CCGCCATAGGCCCCAGGAACAGCTGCGCTTGCGCATTGGCATCCACACAGGTCCTGTGTGTGCTGGTGTG
  GTAGGGCTAAAGATGCCCCGATACTGCCTCTTTGGAGACACAGTCAACACAGCTTCAAGAATGGAGTCTA
  ATGGAGAAGCCCTCAAGATCCACTTGTCTTCAGAGACCAAGGCTGTGCTGGAAGAGTTCGATGGTTTCGA
  GCTGGAGCTCCGAGGGGATGTGGAAATGAAGGGCAAAGGCAAGGTTCGGACCTATTGGCTCCTGGGGGAG
  CGGGGATGTAGCACTCGAGGCTGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_012613
• Insert Size: 3174 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012613.1, NP_036745.1
• RefSeq Size: 4068 bp
• RefSeq ORF: 3174 bp
• Locus ID: 24603
• UniProt ID: P18910
• Cytogenetics: 2q34
• Gene Summary: receptor for atrial natriuretic peptide [RGD, Feb 2006]