Atp6v1a (NM_001108318) Rat Untagged Clone

CAT#: RN204905

Atp6v1a (untagged ORF) - Rat ATPase, H+ transporting, lysosomal V1 subunit A (Atp6v1a), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Atp6v1a
• Synonyms: Atp6v1a1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204905 representing NM_001108318
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCTCCAAGCTACCCAAAATCCGTGATGAAGACAAAGAAAGCACATTTGGTTATGTGCATGGAG
  TCTCAGGGCCTGTGGTCACCGCCTGTGACATGGCGGGCGCTGCCATGTACGAGCTGGTGAGAGTGGGGCA
  CAGCGAGCTGGTCGGAGAGATTATCCGACTGGAAGGTGACATGGCCACCATTCAGGTGTACGAGGAAACT
  TCTGGTGTCTCTGTTGGAGACCCTGTACTCCGCACTGGTAAACCTCTCTCGGTAGAACTTGGTCCTGGCA
  TTATGGGAGCCATTTTTGATGGTATTCAGAGACCTCTATCGGATATCAGCAGTCAGACCCAAAGTATCTA
  CATCCCCAGAGGAGTAAATGTGTCTGCTCTTAGCAGAGATATCAAATGGGAGTTTATACCCAGCAAAAAC
  CTACGGGTTGGTAGTCATATCACTGGTGGAGATATTTATGGGATTGTCAATGAGAACTCGCTCATCAAAC
  ACAAAATCATGCTACCCCCACGCAGCCGAGGAAGTGTGACTTACATCGCACCGCCTGGGAACTACGATGC
  CTCTGATGTCGTCCTGGAGCTTGAATTTGAAGGTGTGAAGGAGAAGTTGAGCATGGTCCAAGTGTGGCCT
  GTGCGTCAGGTGCGGCCTGTCACTGAAAAGCTGCCAGCCAACCACCCCTTGCTTACTGGCCAGAGAGTGC
  TCGATGCCCTTTTCCCGTGTGTTCAGGGAGGAACTACTGCTATTCCAGGTGCTTTTGGCTGTGGGAAGAC
  TGTGATTTCCCAGTCTCTATCCAAGTACTCCAACAGCGATGTGATTATCTATGTCGGCTGCGGTGAAAGA
  GGGAATGAGATGTCAGAAGTTCTCCGGGACTTCCCTGAGCTCACTATGGAAGTTGATGGTAAAGTAGAGT
  CCATCATGAAGAGGACAGCGCTGGTAGCCAATACCTCCAATATGCCTGTGGCTGCAAGAGAGGCCTCCAT
  TTACACTGGAATTACACTATCAGAATACTTCCGTGACATGGGGTACCACGTCAGTATGATGGCTGACTCT
  ACCTCTAGATGGGCTGAGGCCCTCAGAGAAATCTCTGGTCGCTTAGCTGAAATGCCTGCAGATAGTGGAT
  ACCCTGCATACCTTGGTGCCCGACTGGCTTCTTTCTATGAGCGAGCAGGCAGAGTGAAATGTCTTGGAAA
  CCCTGAGAGAGAAGGGAGTGTCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCA
  GTCACATCTGCTACTCTGGGTATTGTTCAGGTGTTCTGGGGCTTGGATAAGAAGCTAGCTCAGCGCAAGC
  ACTTCCCGTCCGTCAACTGGCTCATTAGCTACAGCAAGTACATGCGCGCCCTGGACGAGTACTATGACAA
  ACACTTCACAGAGTTCGTGCCTCTGAGGACCAAAGCTAAGGAGATTCTGCAGGAAGAGGAGGATCTGGCG
  GAAATCGTGCAGCTCGTGGGAAAGGCGTCTTTAGCAGAGACAGATAAAATCACCCTGGAGGTAGCAAAAC
  TTATCAAAGATGACTTCCTACAACAAAATGGGTACACTCCTTATGACAGGTTCTGTCCATTCTATAAGAC
  GGTGGGGATGCTGTCCAACATGATTTCATTCTATGATATGGCCCGCCGGGCTGTGGAGACCACCGCCCAG
  AGTGACAATAAGATCACATGGTCCATTATCCGTGAGCACATGGGGGAGATTCTCTATAAACTTTCCTCCA
  TGAAATTCAAGGATCCAGTGAAGGATGGCGAGGCAAAGATCAAGGCCGACTACGCACAGCTTCTTGAAGA
  TATGCAGAACGCATTCCGTAGCCTGGAAGATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001108318
• Insert Size: 1854 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108318.2, NP_001101788.1
• RefSeq Size: 4069 bp
• RefSeq ORF: 1854 bp
• Locus ID: 685232
• Cytogenetics: 11q21