Itch (NM_001005887) Rat Untagged Clone

CAT#: RN204734

Itch (untagged ORF) - Rat itchy E3 ubiquitin protein ligase homolog (mouse) (Itch), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Itch
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204734 representing NM_001005887
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTAGTCTGACCATGAAATCTCAACTTCAGATTACTGTCATCTCAGCCAAACTTAAAGAAAATAAAA
  AGAATTGGTTTGGACCAAGTCCTTATGTAGAAGTCACAGTAGATGGGCAGTCAAAGAAGACAGAAAAATG
  CAACAATACAAACAGCCCCAAGTGGAAGCAGCCCCTCACAGTTATCGTTACCCCTATGAGTAAATTATGT
  TTTCGTGTGTGGAGTCACCAGACCCTGAAATCTGATGTTTTATTGGGAACTGCTGGATTAGATATTTATG
  AAACATTAAAGTCAAACAATATGAAACTTGAGGAAGTAGTTATGACTTTGCAGCTTGTAGGTGACAAAGA
  GCCAACAGAGACAATGGGAGATTTGTCAGTTTGTCTTGATGGGCTGCAAGTAGAAGCTGAAGTTGTTACT
  AATGGTGAAACTTCATGCTCAGAGGGTGCTACACAGAATGACGCTGGCTGCAGACCCAAAGATGAAACAA
  GAGTGAGCACAAATGGATCAGAGGACCCTGAGGTTGCAGCCTCGGGGGAGAACAAGAGGGCCAATGGGAA
  CAATTCTCCATCCCTTTCAAATGGTGGTTTTAAGCCTTCTAGACCTCCTAGACCTTCAAGACCACCTCCA
  CCCACCCCACGAAGACCAGCTTCTGTCAATGGCTCACCATCCACGAACTCTGACAGCGATGGATCTAGCA
  CAGGCTCTTTGCCACCCACAAATACAAATGTAAATTCAAGTGCATCGGAAGGAGCAGCATCTGGGTTAAT
  AATTCCTCGTACCATCTCTGGGGGCTCAGGCCCTAGGCCGCTGAATACTGTCAGTCAAGCTCCCCTCCCA
  CCTGGGTGGGAGCAGAGAGTAGATCAGCATGGGCGTGCTTACTATGTAGACCATGTTGAAAAGCGAACAA
  CACGGGATAGACCGGAACCTCTACCTCCTGGCTGGGAACGGCGTGTGGACAATATGGGACGTATTTATTA
  TGTTGACCATTTCACGAGAACCACAACATGGCAGAGGCCGACCTTGGAATCTGTCCGGAACTATGAGCAG
  TGGCAGCTCCAGCGTAGTCAACTTCAGGGAGCAATGCAGCAGTTTAACCAGAGATTCATCTTTGGGAATC
  AAGATTTGTTTGCTACATCACAAAACAAAGAGTTTGATCCCCTTGGTCCTTTGCCCCCTGGATGGGAGAA
  GAGAACTGATAGCAACGGCAGAGTGTATTTTGTCAACCACAACACTCGGATTACACAGTGGGAAGACCCC
  AGAAGCCAAGGTCAGTTAAATGAAAAGCCCTTACCAGAAGGCTGGGAAATGCGATTCACAGTGGATGGAA
  TTCCATATTTTGTGGACCACAATAGAAGAGCAACTACCTACATAGATCCACGCACAGGAAAGTCAGCCTT
  AGACAACGGACCCCAGATAGCCTATGTTCGGGACTTCAAGGCAAAAGTTCAGTATTTCCGGTTCTGGTGT
  CAGCAACTGGCCATGCCACAGCATATAAAGATCACAGTGACAAGAAAAACATTGTTTGAGGATTCCTTTC
  AACAGATCATGAGCTTCAGTCCGCAAGATCTGAGAAGACGTTTGTGGGTGATTTTCCCAGGAGAAGAAGG
  TTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAATGTAT
  TGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCCTGCAGATAAACCCCGCTTCTTATATCAACCCAG
  ACCACCTGAAATACTTCCGTTTTATTGGCAGATTTATTGCCATGGCTCTATTCCATGGGAAATTCATAGA
  TACCGGATTTTCTTTACCATTCTATAAACGTATCTTGAATAAACCAGTGGGACTTAAGGATTTAGAATCT
  ATCGATCCAGAATTTTACAATTCTCTCATCTGGGTTAAAGAAAACAATATCGAGGAATGTGGTTTGGAAA
  TGTACTTCTCAGTTGATAAAGAAATTCTGGGTGAAATTAAGAGTCATGATTTGAAACCCAATGGTGGCAA
  TATTCTGGTGACAGAAGAAAATAAGGAAGAGTACATCAGGATGGTCGCTGAGTGGAGGTTGTCTCGGGGA
  GTTGAAGAGCAGACACAAGCTTTCTTTGAGGGCTTTAATGAAATTCTTCCTCAGCAATATTTGCAATATT
  TTGATGCGAAGGAATTAGAGGTCCTTTTGTGTGGGATGCAGGAGATCGATTTGAATGACTGGCAGAGACA
  CGCCATCTACCGTCACTATACCAGGACGAGCAAGCAGATCGTGTGGTTTTGGCAGTTTGTTAAAGAAATT
  GATAACGAGAAGAGGATGCGACTTCTGCAGTTTGTTACCGGAACCTGCCGATTGCCAGTGGGAGGATTTG
  CTGACCTCATGGGGAGTAATGGACCACAGAAGTTCTGCATCGAAAAAGTTGGCAAAGAAAATTGGTTACC
  CAGAAGCCATACTTGTTTTAACCGCCTGGACCTCCCACCCTACAAGAGCTATGAGCAACTGAAGGAAAAG
  CTGTTATTTGCCATTGAAGAAACTGAAGGATTTGGACAAGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005887
• Insert Size: 2565 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005887.1, NP_001005887.1
• RefSeq Size: 2565 bp
• RefSeq ORF: 2565 bp
• Locus ID: 311567
• Cytogenetics: 3q41